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Fabrication, characterization and mosquito larvicidal bioassay of silver nanoparticles synthesized from aqueous fruit extract of putranjiva, Drypetes roxburghii (Wall.)

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Abstract

Highly stable nanoparticles of metallic silver with average dimension of 26.6 nm were synthesized by a simple, cost-effective, reproducible and previously unexploited biogenic source viz. dried green fruits of Drypetes roxburghii (Wall.) (common name putranjiva). The as-synthesized silver nanoparticles (Ag NP) were characterized by their characteristic surface plasmon resonance absorption spectra, X-ray diffraction analysis, energy dispersive X-ray analysis and selected area electron diffraction study. The morphology of the particles was determined by high-resolution transmission electron microscopy. Fourier transform infrared analysis focuses some light on the chemical framework that stabilizes the nanoparticles. The analyses of the phytochemicals present in the fruit extract of the plant were also performed following standard protocol. Mosquito larvicidal bioassay with the Ag NPs was carried out with two mosquitoes, namely Anopheles stephensi Liston and Culex quinquefasciatus Say. The results show impressive mortality rate even at too low concentration of nanoparticle. Toxicity test on non-target organism shows no harmful effect during the study period.

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Acknowledgments

Financial support from University Grants commission (UGC) is gratefully acknowledged. KMH thanks UGC for providing her research fellowship (no. F.17-8/08 (SA-I)). The authors are indebted to Dr. Anupam Ghosh of Bankura Christian College, Bankura, W.B. and Mr. Subhasish Roy of Indian Association for Cultivation of Science for their invaluable cooperation in the FTIR and XRD measurements, respectively.

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Authors and Affiliations

  1. Mosquito & Microbiology Research Units, Parasitology Laboratory, Department of Zoology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India

    Koyel Mallick Haldar & Goutam Chandra

  2. Department of Chemistry (UG & PG), Vivekananda Mahavidyalaya Burdwan, Sripally, Burdwan, 713103, West Bengal, India

    Basudeb Haldar

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  1. Koyel Mallick Haldar
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  2. Basudeb Haldar
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Correspondence to Goutam Chandra.

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Haldar, K.M., Haldar, B. & Chandra, G. Fabrication, characterization and mosquito larvicidal bioassay of silver nanoparticles synthesized from aqueous fruit extract of putranjiva, Drypetes roxburghii (Wall.). Parasitol Res 112, 1451–1459 (2013). https://doi.org/10.1007/s00436-013-3288-4

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  • DOI: https://doi.org/10.1007/s00436-013-3288-4

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