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Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae

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Abstract

Chrysosporium tropicum is a pathogenic fungus. It is known to be an effective mosquito control agent. In the present study, we have synthesized the silver and gold nanoparticles using C. tropicum. These nanoparticles have been characterized through Microscan reader, X-ray diffractometer, transmission electron microscopy, and further confirmed by scanning electron microscopy. The characterization study confirmed the spherical shape and size (2–15 and 20–50 nm) of gold and silver nanoparticles. These silver and gold nanoparticles have been tested as a larvicide against the Aedes aegypti larvae. The larvicidal efficacy was noted when performed against all instars of A. aegypti at six different log concentrations, and significant results could be observed. The gold nanoparticles used as an efficacy enhancer have shown mortality at three times higher concentration than the silver nanoparticles. The larval mortality was observed after different time of exposures. The mortality values were obtained using the probit analysis. The larvae of A. aegypti were found to be highly susceptible for the silver nanoparticles. The second instar larvae have shown 100% mortality against the silver nanoparticles after 1 h, whereas the first, third, and fourth instars have shown efficacy (LC50 = 3.47, 4, and 2; LC90 = 12.30, 8.91, and 4; LC99 = 13.18, 13.18, and 7.58, respectively) after 1 h. The results could suggest that the use of fungus C. tropicum, silver, and gold nanoparticles is a rapid, environmentally safer, and greener approach for mosquito control. This could lead us to a new possibility in vector control strategy.

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Acknowledgments

We are sincerely grateful to Prof. P. S. Satsangi Sahab, Chairman of Advisory Committee on Education, Dayalbagh Educational Institute. We thank Prof. V. G. Das, Director, Dayalbagh Educational Institute for providing support and encouragements for the work. We also wish to thank DST-FIST (2003–2008) Government of India for facilitating equipments in our laboratory. We wish to thank Prof. Sahab Das, Dept. of Chemistry, Dayalbagh Educational Institute, for providing XRD facilities. We also thank Dr. Shashi Wadhawa (AIIMS, Delhi) and Dr. M. Shayam Prasad for SEM (NIO, Goa) for their generous help with TEM and SEM, respectively.

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  1. Department of Zoology, Environmental and Advanced Parasitology and Vector Control Biotechnology Laboratories, Dayalbagh Educational Institute, Dayalbagh, Agra, 282 005, India

    Namita Soni & Soam Prakash

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  1. Namita Soni
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  2. Soam Prakash
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Correspondence to Soam Prakash.

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Soni, N., Prakash, S. Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae. Parasitol Res 110, 175–184 (2012). https://doi.org/10.1007/s00436-011-2467-4

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