Abstract
Key message
γ-HCH was successfully degraded using LinA-expressed transgenic hairy root cultures of Cucurbita moschata . Fusing an endoplasmic reticulum-targeting signal peptide to LinA was essential for stable accumulation in the hairy roots.
Abstract
The pesticide γ-hexachlorocyclohexane (γ-HCH) is a persistent organic pollutant (POP) that raises public health and environmental pollution concerns worldwide. Although several isolates of γ-HCH-degrading bacteria are available, inoculating them directly into γ-HCH-contaminated soil is ineffective because of the bacterial survival rate. Cucurbita species incorporate significant amounts of POPs from soils compared with other plant species. Here, we describe a novel bioremediation strategy that combines the bacterial degradation of γ-HCH and the efficient uptake of γ-HCH by Cucurbita species. We produced transgenic hairy root cultures of Cucurbita moschata that expressed recombinant bacterial linA, isolated from the bacterium Sphingobium japonicum UT26. The LinA protein was accumulated stably in the hairy root cultures by fusing an endoplasmic reticulum (ER)-targeting signal peptide to LinA. Then, we demonstrated that the cultures degraded more than 90 % of γ-HCH (1 ppm) overnight and produced the γ-HCH metabolite 1,2,4-trichlorobenzene, indicating that LinA degraded γ-HCH. These results indicate that the gene linA has high potential for phytoremediation of environmental γ-HCH.
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Acknowledgments
We thank Dr. Y. Wakasa for performing the deglycosylation analysis and Dr. S. Hayashi and Dr. H. Takahashi for performing microscopy and immunostaining; Ms. T. Tachibana, Ms. I. Kawaguchi, and Ms. K. Masuda for maintaining the plant cultures; Ms. Y. Ishikawa (Organochemicals Division, National Institute for Agro-Environmental Sciences) for preparing samples for the GC–MS analysis; and Dr. A. Okuzaki and Dr. S. Takahashi for their fruitful discussions. Enago (http://enago.jp) corrected the manuscript.
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Communicated by H. Ebinuma.
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Nanasato, Y., Namiki, S., Oshima, M. et al. Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA. Plant Cell Rep 35, 1963–1974 (2016). https://doi.org/10.1007/s00299-016-2011-1
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DOI: https://doi.org/10.1007/s00299-016-2011-1