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Hairy roots — a short cut to transgenic root nodules

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Abstract

To facilitate molecular studies of symbiotic nitrogen fixation a procedure for rapid production of transgenic root nodules was established on the legumeLotus corniculatus (Bird'sfoot trefoil). Regeneration of transgenic plants is not required as transgenic nodules are formed onAgrobacterium rhizogenes incited roots inoculated withRhizobium. Easy identification of transformed roots is possible using a set ofA. rhizogenes acceptor strains carrying assayable marker genes such as chloramphenicol acetyltransferase (CAT), β-glucuronidase (GUS), or luciferase (LUC) under control of the cauliflower mosaic virus (CaMV) 35S promoter. Counterselection ofA. rhizogenes after infection of plants was improved using an auxotrophy marker.

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Abbreviations

CAT:

chloramphenicol acetyltransferase

GUS:

β-glucuronidase

LUC:

luciferase

Ri:

root inducing

TL:

left T-DNA

CaMV:

Cauliflower Mosaic Virus

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Authors and Affiliations

  1. Department of Molecular Biology and Plant Physiology, University of Aarhus, C.F. Møllers Allé 130, DK-8000, Aarhus C, Denmark

    Jørgen Hansen, Jan-Elo Jørgensen, Jens Stougaard & Kjeld A. Marcker

Authors
  1. Jørgen Hansen
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  2. Jan-Elo Jørgensen
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  3. Jens Stougaard
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  4. Kjeld A. Marcker
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Communicated by H. Lörz

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Hansen, J., Jørgensen, JE., Stougaard, J. et al. Hairy roots — a short cut to transgenic root nodules. Plant Cell Reports 8, 12–15 (1989). https://doi.org/10.1007/BF00735768

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  • DOI: https://doi.org/10.1007/BF00735768

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