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Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites

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Abstract

Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20–30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.

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Acknowledgments

This study was supported by the National Research Council of Argentina (Grant PIP CONICET 0184 to A.A.G.). The authors thank the German research council (Deutsche Forschungsgemeinschaft, DFG) for partial financial support of this work.

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Authors and Affiliations

  1. Interdisciplinary Materials Group-IESIING-UCASAL, INTECIN UBA-CONICET, A4400EDD, Salta, Argentina

    Gabriela E. Vargas, Luis A. Haro Durand, Vanesa Cadena & Alejandro A. Gorustovich

  2. School of Natural Sciences, National University of Salta, A4408FVY, Salta, Argentina

    Gabriela E. Vargas, Vanesa Cadena, Marcela Romero & Rosa Vera Mesones

  3. Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany

    Mirza Mačković, Stefanie Spallek, Erdmann Spiecker & Aldo R. Boccaccini

  4. Center for Nanoanalysis and Electron Microscopy (CENEM), University of Erlangen-Nuremberg, 91058, Erlangen, Germany

    Mirza Mačković, Stefanie Spallek & Erdmann Spiecker

Authors
  1. Gabriela E. Vargas
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  2. Luis A. Haro Durand
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  3. Vanesa Cadena
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  4. Marcela Romero
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  5. Rosa Vera Mesones
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  9. Aldo R. Boccaccini
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  10. Alejandro A. Gorustovich
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Correspondence to Alejandro A. Gorustovich.

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Vargas, G.E., Haro Durand, L.A., Cadena, V. et al. Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites. J Mater Sci: Mater Med 24, 1261–1269 (2013). https://doi.org/10.1007/s10856-013-4892-7

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  • DOI: https://doi.org/10.1007/s10856-013-4892-7

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