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Multiple-line cross QTL mapping for biomass yield and plant height in triticale (× Triticosecale Wittmack)

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QTL mapping in multiple families identifies trait-specific and pleiotropic QTL for biomass yield and plant height in triticale.

Abstract

Triticale shows a broad genetic variation for biomass yield which is of interest for a range of purposes, including bioenergy. Plant height is a major contributor to biomass yield and in this study, we investigated the genetic architecture underlying biomass yield and plant height by multiple-line cross QTL mapping. We employed 647 doubled haploid lines from four mapping populations that have been evaluated in four environments and genotyped with 1710 DArT markers. Twelve QTL were identified for plant height and nine for biomass yield which cross-validated explained 59.6 and 38.2 % of the genotypic variance, respectively. A major QTL for both traits was identified on chromosome 5R which likely corresponds to the dominant dwarfing gene Ddw1. In addition, we detected epistatic QTL for plant height and biomass yield which, however, contributed only little to the genetic architecture of the traits. In conclusion, our results demonstrate the potential of genomic approaches for a knowledge-based improvement of biomass yield in triticale.

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Acknowledgments

This research was funded by the German Federal Ministry of Education and Research (BMBF) under the promotional reference 0315414A. This publication reflects the views only of the authors. We acknowledge the handling of the funding by the Project Management Organisation Jülich (PtJ). We thank Angela Harmsen for excellent technical assistance in the laboratory and Agnes Rölfing-Finze, Hans Häge, Jacek Till and Justus von Kittlitz for their outstanding work in the greenhouse and field.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

The authors declare that the experiments comply with the current laws of Germany.

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Authors and Affiliations

  1. State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany

    Katharina V. Alheit, Hans Peter Maurer, Manje Gowda, Volker Hahn & Tobias Würschum

  2. Competence Centre of Applied Agricultural Engineering, University of Applied Sciences Osnabrück, Sedanstrasse 26, 49076, Osnabrück, Germany

    Lucas Busemeyer & Arno Ruckelshausen

  3. Crop Genetics and Breeding Department, China Agricultural University, Beijing, 100193, China

    Wenxin Liu

  4. Saatzucht Dr. Hege GbR Domäne Hohebuch, 74638, Waldenburg, Germany

    Sigrid Weissmann

  5. Leibnitz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany

    Jochen C. Reif

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  1. Katharina V. Alheit
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  2. Lucas Busemeyer
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  3. Wenxin Liu
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  4. Hans Peter Maurer
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  5. Manje Gowda
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  6. Volker Hahn
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  7. Sigrid Weissmann
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  8. Arno Ruckelshausen
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  9. Jochen C. Reif
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  10. Tobias Würschum
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Corresponding author

Correspondence to Tobias Würschum.

Additional information

Communicated by P. Langridge.

K. V. Alheit, L. Busemeyer and W. Liu contributed equally to this work.

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Alheit, K.V., Busemeyer, L., Liu, W. et al. Multiple-line cross QTL mapping for biomass yield and plant height in triticale (× Triticosecale Wittmack). Theor Appl Genet 127, 251–260 (2014). https://doi.org/10.1007/s00122-013-2214-6

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