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Transferability of SSR markers among wheat, rye, and triticale

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Abstract

Simple sequence repeat (SSR) markers are a valuable tool for many purposes, such as mapping, fingerprinting, and breeding. However, they are only available in some economically important crops because of the high cost and labor intensity involved in their development. Comparative mapping reveals a high degree of colinearity between closely related species, which allows the exchange of markers between them. Our objective was to examine the transferability of SSR markers among wheat (Triticum aestivum L.), rye (Secale cereale L.), and triticale (X Triticosecale Wittmack). One hundred forty-eight wheat and 28 rye SSR markers were used to amplify genomic DNA extracted from five lines each of wheat, rye, and triticale. Transferability of wheat SSR markers to rye was 17%, whereas 25% of rye markers were amplifiable in wheat. In triticale, 58% and 39% transferability was achieved for wheat and rye markers, respectively. Wheat markers gave an average of 2.6, 2.7, and 2.4 polymorphic bands in wheat, rye, and triticale, respectively, while rye markers gave an average of 2.0 in rye and none in wheat and triticale. These transferable markers can now be exploited for further genetic and breeding studies in these species.

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References

  • Akkaya M, Bhagwat A, Cregan P (1992) Length polymorphisms of simple-sequence repeat DNA in soybean. Genetics 132:1131–1139

    CAS  PubMed  Google Scholar 

  • Alvarez AE, van de Wiel CCM, Smulders MJM, Vosman B (2001) Use of microsatellites to evaluate genetic diversity and species relationships in the genus Lycopersicon. Theor Appl Genet 103:1283–1292

    Article  CAS  Google Scholar 

  • Bowcock AM, Ruiz-Linares A, Tomfohrde A, Minch E, Kidd JR, Cavalli-Sforza LL (1994) High-resolution human evolutionary trees from polymorphic microsatellites. Nature 368:455–457

    CAS  PubMed  Google Scholar 

  • Brown M, Hopkins M, Mitchell S, Smior M, Wang T, Duncan R, Gonfzle-Candelas F, Kresovich (1996) Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum [Sorghum bicolor (L.) Moench)]. Theor Appl Genet 93:190–198

    Article  CAS  Google Scholar 

  • Caudrado A, Schearzacher T (1998) The chromosomal organization of simple sequence repeats in wheat and rye genomes. Chromosoma 107:587–594

    Article  CAS  PubMed  Google Scholar 

  • Cordeiro GM, Taylor GO, Henry RJ (2000) Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species. Plant Sci 155:161–168

    CAS  PubMed  Google Scholar 

  • Cregan PB, Jarvick T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lohnes DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490

    CAS  Google Scholar 

  • Devos KM, Gale MD (1993) Extended genetics maps of homeologous group 3 chromosomes of wheat, rye and barley. Theor Appl Genet 85:649–652

    CAS  Google Scholar 

  • Devos KM, Millan T, Gale MD (1993) Comparative RFLP maps of the homeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85:784–792

    CAS  Google Scholar 

  • Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arús P, Laigret F (2002) Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.) Theor Appl Genet 105:127–138

  • Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185

    Article  CAS  Google Scholar 

  • Hackauf B, Wehling P (2002) Identification of microsatellite polymorphisms in an expressed portion of the rye genome. Plant Breed 121:17–25

    CAS  Google Scholar 

  • Hernández P, Oorado G, Laurie D, Martin A, Snape J (2001) Microsatellites and RFLP probes from maize are efficient sources of molecular markers for the biomass energy crop Miscanthus. Theor Appl Genet 102:616–622

    Article  Google Scholar 

  • Hernández P, Laurie DA, Martin A, Snape JW (2002) Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritodeum. Theor Appl Genet 104:735–739

    Article  Google Scholar 

  • Horlein A, Valentine J (1995) Triticale (× Triticosecale). In: Williams JT (ed) Cereals and pseudocereals. Chapman and Hall, New York, pp 187–221

  • Korzun V, Malyshev S, Kartel N, Westermann T, Weber WE, Börner A (1998) A genetic linkage map of rye (Secale cereale L.). Theor Appl Genet 96:203–208

    Article  CAS  Google Scholar 

  • Korzun V, Malyshev S, Voylokov AV, Boerner A (2001) A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor Appl Genet 102:709–717

    CAS  Google Scholar 

  • Liu ZW, Biyashev RM, Saghai-Maroof MA (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet 93:869–876

    CAS  Google Scholar 

  • Ozkan H, Levy AA, Feldman M (2001) Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group. Plant Cell 13:1735–1747

    CAS  PubMed  Google Scholar 

  • Peakall R, Gilmore S, Keys W, Morgante M, Rafaske A (1998) Cross-species amplification of soybean (Glycine max) simple sequence repeat (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol Biol Evol 15:1275–1287

    CAS  PubMed  Google Scholar 

  • Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592

    CAS  Google Scholar 

  • Röder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellite wheat. Mol Genet Genomics 246:327–333

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rongwen J, Akkaya MS, Bhagwat AA, Lavi U, Cregan PB (1995) The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90:43–48

    CAS  Google Scholar 

  • Saal B, Wricke G (1999) Development of simple sequence repeat markers in rye (Secale cereale L.). Genome 42:964–972

    CAS  PubMed  Google Scholar 

  • Saghai-Maroof MA, Biyashev RM, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA 91:5466–5470

    CAS  PubMed  Google Scholar 

  • Senior ML, Murphy JP, Goodman MM, Stuber CW (1998) Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system. Crop Sci 38:1088–1098

    Google Scholar 

  • Sharopova N, McMullen MD, Schultz L (2002) Development and mapping of SSR markers for maize. Plant Mol Biol 48:463–481

    Article  CAS  PubMed  Google Scholar 

  • Soltis DE, Soltis P (1999) Polyploidy: recurrent formation and genome evolution. Trends Ecol Evol 14:348–352

    PubMed  Google Scholar 

  • Song QJ, Fickus EW, Cregan PB (2002) Characterization of trinucleotide SSR motifs in wheat. Theor Appl Genet 104:286–293

    Article  Google Scholar 

  • Sourdille P, Tavaud M, Charmet G, Bernard M (2001) Transferability of wheat microsatellites to diploid Triticeae species carrying the A, B and D genomes. Theor Appl Genet 103:346–352

    CAS  Google Scholar 

  • Stachel M, Lelley T, Grausgruber H, Vollmann J (2000) Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use. Theor Appl Genet 100:242–248

    Article  Google Scholar 

  • Temnykh S, DeClerck G, Lukashova A (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res 11:1441–1452

    CAS  PubMed  Google Scholar 

  • Tikhanov AP, SanMiguel PJ, Nakajima Y, Gorenstein NM, Bennetzen JF, Avramova Z (1999) Colinearity and its exceptions in orthologous adh regions of maize and sorghum. Proc Natl Acad Sci USA 96:7409–7414

    CAS  PubMed  Google Scholar 

  • US Wheat and Barley Scab Initiative (2001) Preliminary BARC SSR maps and primer pairs. http://www.scabusa.org/pdfs/BARC_SSRs_011101.html

  • Van Deynze AE, Sorrells ME, Park WD, Ayres NM, Fu H, Cartinhour SW, Paul E, McCouch SR (1998) Anchor probes for comparative mapping of grass genera. Theor Appl Genet 97:356–369

    Article  Google Scholar 

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Acknowledgements

The authors would like to thank Drs. Perry Cregan and Qijian Song (Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, Md.) for kindly providing the sequence information for the BARC markers, and the Ministry of University Affairs, Thailand, for partial support of this research.

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Authors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583-0915, USA

    C. Kuleung, P. S. Baenziger & I. Dweikat

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  1. C. Kuleung
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  2. P. S. Baenziger
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  3. I. Dweikat
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Correspondence to I. Dweikat.

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Nebraska Agricultural Research Division, Journal Series No. 14243

Communicated by B. Friebe

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Kuleung, C., Baenziger, P.S. & Dweikat, I. Transferability of SSR markers among wheat, rye, and triticale. Theor Appl Genet 108, 1147–1150 (2004). https://doi.org/10.1007/s00122-003-1532-5

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  • DOI: https://doi.org/10.1007/s00122-003-1532-5

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