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Optimization of Endoglucanase and Xylanase Activities from Fusarium verticillioides for Simultaneous Saccharification and Fermentation of Sugarcane Bagasse

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Abstract

Enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. Thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. The production of endoglucanase and xylanase activites from F. verticillioides were optimized in a factorial design (25) followed by a CCDR design. Endoglucanase and xylanase activities increased from 2.8 to 8.0 U/mL and from 13.4 to 114 U/mL, respectively. The optimal pH and temperature were determined for endoglucanase (5.6, 80 °C), cellobiase (5.6, 60 °C), FPase (6.0, 55 °C) and xylanase (7.0, 50 °C). The optimized crude extract was applied in saccharification and fermentation of sugarcane bagasse from which 9.7 g/L of ethanol was produced at an ethanol/biomass yield of 0.19.

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References

  1. Visser, E. M., Filho, D. O., Martins, M. A., & Steward, B. L. (2011). Biomass Bioenergy, 35, 489–494.

    Article  CAS  Google Scholar 

  2. Lynd, L. R., Weimer, P. J., Van Zyl, W. H., & Pretorius, I. S. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.

    Article  CAS  Google Scholar 

  3. Rowell, M. R., Pettersen, R., Han, J. S., Rowell, J. S., Tshabalala, M. A. (2005). Handbook of wood chemistry and wood composites, CRC.

  4. Saha, B. C. (2000). Biotechnology Advances, 18, 403–423.

    Article  CAS  Google Scholar 

  5. Margeot, A., Hahn-Hagerdal, B., Edlund, M., Slade, R., & Monot, F. (2009). Current Opinion in Biotechnology, 20, 372–380.

    Article  CAS  Google Scholar 

  6. Liming, X., & Xueliang, S. (2004). Bioresource Technology, 91, 259–262.

    Article  Google Scholar 

  7. Falkoski, D. L., Guimarães, V. M., Almeida, M. N., Alfenas, A. C., Colodette, J. L., & de Rezende, S. T. (2013). Bioresource Technology, 103, 296–305.

    Article  Google Scholar 

  8. Visser, H., Joosten, V., Punt, P. J., Gusakov, A. V., Olson, P. T., Joosten, R., et al. (2011). Industrial Biotechnology, 7, 214–223.

    Article  CAS  Google Scholar 

  9. Martins, L. F., Kolling, D., Camassola, M., Dillon, A. J. P., & Ramos, L. P. (2008). Bioresource Technology, 99, 1417–1424.

    Article  CAS  Google Scholar 

  10. Soni, R., Nazir, A., & Chadha, B. S. (2010). Industrial Crop Production, 31, 277–283.

    Article  CAS  Google Scholar 

  11. Ravalason, H., Grisel, S., Chevret, D., Favel, A., Berrin, J.-G., Sigoillot, J.-C., et al. (2012). Bioresource Technology, 114, 589–596.

    Article  CAS  Google Scholar 

  12. Almeida, M. N., Guimarães, V. M., Bischoff, K., Falkoski, D. L., Pereira, O. L., Gonçalves, D. S. P. O., et al. (2011). Applied Biochemistry and Biotechnology, 165, 594–610.

    Article  Google Scholar 

  13. Almeida, M. N., Falkoski, D. L., Guimarães, V. M., Ramos, H. J. O., Visser, E. M., Maitan-Alfenas, G. P., et al. (2013). Bioresource Technology, 143, 413–422.

    Article  Google Scholar 

  14. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  15. Mandels, M., Weber, J. (1969). The production of cellulases, American Chemical Society

  16. McIlvaine, T. (1921). Journal of Biological Chemistry, 49, 183–186.

    CAS  Google Scholar 

  17. Falkoski, D. L., Guimarães, V. M., Almeida, M. N., Alfenas, A. C., Colodette, J. L., & de Rezende, S. T. (2012). Applied Biochemistry and Biotechnology, 166, 1586–1603.

    Article  CAS  Google Scholar 

  18. de Castro, A. M., de Albuquerque de Carvalho, M. L., Leite, S. G. F., & Pereira, N., Jr. (2010). Journal of Industrial Microbiology and Biotechnology, 37, 151–158.

    Article  CAS  Google Scholar 

  19. Hideno, A., Inoue, H., Tsukahara, K., Yano, S., Fang, X., Endo, T., et al. (2011). Enzyme and Microbial Technology, 48, 162–168.

    Article  CAS  Google Scholar 

  20. Camassola, M., & Dillon, A. J. P. (2007). Journal of Applied Microbiology, 103, 2196–2204.

    Article  CAS  Google Scholar 

  21. Fang, X., Yano, S., Inoue, H., & Sawayama, S. (2008). Journal of Bioscience and Bioengineering, 106, 115–120.

    Article  CAS  Google Scholar 

  22. Fekete, E., Seiboth, B., Kubicek, C. P., Szentirmai, A., & Karaffa, L. (2008). PNAS, 105, 7141–7146.

    Article  CAS  Google Scholar 

  23. Maeda, R. N., Da Silva, M. M. P., Santa Anna, L. M. M., & Pereira, N., Jr. (2010). Applied Biochemistry and Biotechnology, 161, 411–422.

    Article  CAS  Google Scholar 

  24. Gusakov, A. V. (2011). Trends in Biotechnology, 29, 419–425.

    Article  CAS  Google Scholar 

  25. Wen, Z., Liao, W., & Chen, S. (2005). Bioresource Technology, 96, 491–499.

    Article  CAS  Google Scholar 

  26. Camassola, M., & Dillon, A. J. P. (2009). Industrial Crop Production, 29, 642–647.

    Article  CAS  Google Scholar 

  27. Dedavid e Silva, L. A., Cortez Lopes, F., Terra Silveira, S., & Brandelli, A. (2009). Applied Biochemistry and Biotechnology, 152, 295–305.

    Article  CAS  Google Scholar 

  28. Jiang, X., Geng, A., He, N., & Li, Q. (2010). Journal of Bioscience and Bioengineering, 111, 121–127.

    Article  Google Scholar 

  29. Berlin, A., Maximenko, V., Gilkes, N., & Saddler, J. (2007). Biotechnology and Bioengineering, 97, 287–296.

    Article  CAS  Google Scholar 

  30. Delabona, P. D. S., Pirota, R. D. P. B., Codima, C. A., Tremacoldi, C. R., Rodrigues, A., & Farinas, C. S. (2013). Industrial Crop Production, 42, 236–242.

    Article  CAS  Google Scholar 

  31. Karboune, S., Geraert, P.-A., & Kermasha, S. (2008). Journal of Agricultural and Food Chemistry, 56, 903–909.

    Article  CAS  Google Scholar 

  32. Shuyan, L., Xinyuan, D., Xuemei, L., & Peiji, G. (2006). Chinese Science B, 51, 191–197.

    Article  Google Scholar 

  33. Visser, E. M., Filho, D. O., Tótola, M. R., Martins, M. A., & Guimarães, V. M. (2012). Bioprocess and Biosystems Engineering, 35, 801–807.

    Article  CAS  Google Scholar 

  34. Souza, C. J. A., Costa, D. A., Rodrigues, M. Q. R. B., dos Santos, A. F., Lopes, M. R., Abrantes, A. B. P., et al. (2012). Bioresource Technology, 109, 63–69.

    Article  Google Scholar 

  35. Hernández-Salas, J. M., Villa-Ramírrez, M. S., Veloz-Rendón, J. S., Rivera-Hernández, K. N., González-César, R. A., Plascencia-Espinosa, M. A., et al. (2009). Bioresource Technology, 100, 1238–1245.

    Article  Google Scholar 

  36. Pessani, N. K., Atiyeh, H. K., Wilkins, M. R., Bellmer, D. D., & Banat, I. M. (2011). Bioresource Technology, 102, 10618–10624.

    Article  CAS  Google Scholar 

  37. Geddes, C. C., Mullinnix, M. T., Nieves, I. U., Peterson, J. J., Hoffman, R. W., York, S. W., et al. (2011). Bioresource Technology, 102, 2702–2711.

    Article  CAS  Google Scholar 

  38. Velmurugan, R., & Muthukumar, K. (2011). Bioresource Technology, 102, 7119–7123.

    Article  CAS  Google Scholar 

  39. Ewanick, S., & Bura, R. (2012). Bioresource Technology, 102, 2651–2658.

    Article  Google Scholar 

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Acknowledgments

We thank Dr. Luciano Gomes Fietto for kindly give the yeast used in this study. We also thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais—FAPEMIG and Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq for their financial support and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES for providing scholarships.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Maíra N. de Almeida, Valéria M. Guimarães, Daniel L. Falkoski, Guilherme B. T. Paes, Evan M. Visser & Sebastião T. de Rezende

  2. Departamento de Estatística, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    José Ivo Ribeiro Jr.

  3. Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Rafael F. Alfenas & Olinto L. Pereira

Authors
  1. Maíra N. de Almeida
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  2. Valéria M. Guimarães
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  3. Daniel L. Falkoski
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  4. Guilherme B. T. Paes
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  5. José Ivo Ribeiro Jr.
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  6. Evan M. Visser
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  7. Rafael F. Alfenas
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  8. Olinto L. Pereira
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  9. Sebastião T. de Rezende
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Correspondence to Sebastião T. de Rezende.

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de Almeida, M.N., Guimarães, V.M., Falkoski, D.L. et al. Optimization of Endoglucanase and Xylanase Activities from Fusarium verticillioides for Simultaneous Saccharification and Fermentation of Sugarcane Bagasse. Appl Biochem Biotechnol 172, 1332–1346 (2014). https://doi.org/10.1007/s12010-013-0572-9

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  • DOI: https://doi.org/10.1007/s12010-013-0572-9

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