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Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

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Abstract

The aim of this work was to evaluate the biochemical features of the white-rot fungi Pycnoporus sanguineus cellulolytic complex and its utilization to sugarcane bagasse hydrolysis. When cultivated under submerged fermentation using corn cobs as carbon source, P. sanguineus produced high FPase, endoglucanase, β-glucosidase, xylanase, mannanase, α-galactosidase, α-arabinofuranosidase, and polygalacturonase activities. Cellulase activities were characterized in relation to pH and temperature. β-Glucosidase and FPase activities were higher at 55 °C, pH 4.5, and endoglucanase activity was higher at 60 °C, in a pH range of 3.5–4.0. All cellulase activities were highly stable at 40 and 50 °C through 48 h of pre-incubation. Crude enzymatic extract from P. sanguineus was applied in a saccharification experiment using acid-treated and alkali-treated sugarcane bagasse as substrate, and the hydrolysis yields were compared to that obtained by a commercial cellulase preparation. Reducing sugar yields of 60.4% and 64.0% were reached when alkali-treated bagasse was hydrolyzed by P. sanguineus extract and commercial cellulase, respectively. Considering the glucose production, it was observed that P. sanguineus extract and commercial cellulase ensured yields of 22.6% and 36.5%, respectively. The saccharification of acid-treated bagasse was lower than that of alkali-treated bagasse regardless of the cellulolytic extract. The present work showed that P. sanguineus has a great potential as an enzyme producer for biomass saccharification.

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Acknowledgments

We thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico (CNPq) for the financial support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for providing scholarships.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    Daniel Luciano Falkoski, Valéria Monteze Guimarães, Maíra Nicolau de Almeida & Sebastião Tavares de Rezende

  2. Department of Phytopathology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil

    Acelino Couto Alfenas

  3. Department of Forest Engineering, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil

    Jorge Luiz Colodette

Authors
  1. Daniel Luciano Falkoski
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  2. Valéria Monteze Guimarães
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  3. Maíra Nicolau de Almeida
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  4. Acelino Couto Alfenas
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  5. Jorge Luiz Colodette
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  6. Sebastião Tavares de Rezende
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Correspondence to Sebastião Tavares de Rezende.

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Falkoski, D.L., Guimarães, V.M., de Almeida, M.N. et al. Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification. Appl Biochem Biotechnol 166, 1586–1603 (2012). https://doi.org/10.1007/s12010-012-9565-3

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  • DOI: https://doi.org/10.1007/s12010-012-9565-3

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