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Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream

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Abstract

Background

Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC).

Aim

The purpose of this study was to evaluate the antioxidants effects of n-acetylcysteine (NAC) in colon segments without faecal stream.

Methods

Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann–Whitney test and ANOVA were used for statistical analysis; p < 0.05 was considered significant.

Results

The oxidative DNA damage in colon segments without faecal stream was significantly lower in animals treated with either concentration of NAC than in control group, regardless of the duration of intervention (p < 0.01).

Conclusions

Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.

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Acknowledgments

Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP). Process Number: 2010/12492-7.

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Authors and Affiliations

  1. Post-Graduate Program in Health Science, São Francisco University, Rua Rui Barbosa, 255 apto. 32, Vila Boa Vista, Santo André, São Paulo, CEP: 09190-370, Brazil

    Carlos Augusto Real Martinez

  2. Post-Graduate Program in Health Science, São Francisco University Medical School, Av. São Francisco de Assis, 218, Vila São José, Bragança Paulista, São Paulo, CEP: 12916-900, Brazil

    Marcos Gonçalves de Almeida, Marcelo Lima Ribeiro, Fernando Lorenzetti da Cunha, Murilo Rocha Rodrigues, Daniela Tiemi Sato & José Aires Pereira

  3. State University of Campinas, Av. Antonio dos Santos Carvalhinho, 160, Bcl. 4 apto. 208, Campinas, São Paulo, CEP: 13091-599, Brazil

    Camila Moraes Gonçalves da Silva

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  1. Carlos Augusto Real Martinez
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Correspondence to Carlos Augusto Real Martinez.

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Martinez, C.A.R., de Almeida, M.G., da Silva, C.M.G. et al. Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream. Dig Dis Sci 58, 3452–3459 (2013). https://doi.org/10.1007/s10620-013-2768-9

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  • DOI: https://doi.org/10.1007/s10620-013-2768-9

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