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Coumaric acid ameliorates experimental colitis in rats through attenuation of oxidative stress, inflammatory response and apoptosis

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Abstract

Objective

Due to the high side effects of commonly used drugs and according to the pharmacological properties reported for coumaric acid (CA), this study was designed to determine the impact of CA on acetic acid-induced colitis in rats, considering its possible anti-inflammatory, antioxidant, and anti-apoptotic properties.

Materials and methods

Forty-eight male Wistar rats were divided into 6 equal groups (n = 8). Colitis was induced by acetic acid intrarectally. CA in three different doses (50, 100, and 150 mg/kg) was administrated for 5 days. Finally, the macroscopic and histopathological changes in the colon tissue were examined. The expression of inflammatory and apoptotic genes, including NF-κB, TNF-α, INOS, IL-1β, IL-6, TLR4, Caspase-3, Caspase-8, Bax, Bcl-2 was assessed. In addition, changes in the levels of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), nitrite, and total antioxidant capacity (TAC) were measured in the colon tissue.

Results

Colitis led to a decrease in TAC and the activity levels of CAT and SOD and an increase in the expression of inflammatory and apoptotic genes, MDA, and nitrite levels in the colon. Colitis was also associated with edema and severe damage to the epithelium, infiltration of inflammatory cells, and the presence of ulcers and necrosis in the colon tissue. CA significantly improved the inflammation, oxidative stress, apoptosis, and histopathological indices caused by acetic acid-induced colitis on the colon.

Conclusion

It is concluded that CA probably exerts its positive effects in the management of colitis, through its anti-inflammatory, antioxidant, and anti-apoptotic properties.

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Data availability

Data regarding the present study are available at the Medical Plants Research Center, Shahrekord University of Medical Sciences.

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Acknowledgements

The authors thank the Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran, for supporting this study.

Funding

This study was supported by a research grant (5186) from Shahrekord University of Medical Sciences, Shahrekord, Iran.

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Authors and Affiliations

  1. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Maryam Ghasemi-Dehnoo, Hossein Amini-Khoei, Zahra Lorigooini, Korosh Ashrafi-Dehkordi & Mahmoud Rafieian-Kopaei

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  1. Maryam Ghasemi-Dehnoo
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  2. Hossein Amini-Khoei
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  3. Zahra Lorigooini
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  4. Korosh Ashrafi-Dehkordi
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Contributions

All the authors were involved in the design, Material preparation, data collection and analysis, manuscript writing, and editing. All the authors read and approved the final draft of the manuscript.

Corresponding author

Correspondence to Mahmoud Rafieian-Kopaei.

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Conflict of interest

The authors have no conflicts of interest to declare regarding the study described in this article and the preparation of the manuscript.

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All procedures were carried out under the regulations of the University and the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (ethical code: IR.SKUMS.REC.1399.113) and Guide for the Care and Use of Laboratory Animals (8th edition, National Academies Press).

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Ghasemi-Dehnoo, M., Amini-Khoei, H., Lorigooini, Z. et al. Coumaric acid ameliorates experimental colitis in rats through attenuation of oxidative stress, inflammatory response and apoptosis. Inflammopharmacol 30, 2359–2371 (2022). https://doi.org/10.1007/s10787-022-01074-z

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