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Synthetic seed production by encapsulating nodal segment of Capparis decidua (Forsk.), in vitro regrowth of plantlets and their physio biochemical studies

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Abstract

Synthetic seed technology is an emerging and broadly used technique in the field of plant biotechnology to conserve economically important plants. In the present study, nodal segments of Capparis decidua were entrapped in calcium alginate gel matrix to produce firm and uniform synthetic seeds. 3% sodium alginate and 75 mM calcium chloride were found best for encapsulation. Among all the concentrations and combinations of thidiazuron (TDZ) either singly or with indole -3- acetic acid (IAA) augmented in Murashige and Skoog medium used, TDZ (5.0 µM) + IAA (0.5 µM) was found most effective in conversion of synthetic seeds into plantlets as 79% plantlets were developed on this combination with 13.2 ± 0.87 shoots and 5.5 ± 0.40 cm shoot length after 8 weeks of culture. Further, synthetic seeds stored at low temperature (4 °C) can retain their viability up to 4 weeks and showed maximum conversion rate (93%) into plantlets, when placed back to regeneration medium. Root formation was also occurred in the same regeneration medium and roots were healthy. Plantlets were successfully hardened in culture room in plastic cups filled with sterile vermiculite and after 4 weeks, they were transferred to greenhouse where they exhibited normal growth with 80% survival. Growth parameters were evaluated in micropropagated plants and compared with the seedlings of same age. Effect of different days of acclimatization were also recorded on various physio-biochemical activities and showed a positive response that can be interpreted as better protection mechanism of micropropagated plants against the stress possibly generated due to reactive oxygen species when transferred to ex vitro environment.

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Abbreviations

CaCl2·2H2O:

Calcium chloride

CAT:

Catalase

Chl:

Chlorophyll

IAA:

Indole-3- acetic acid

MS:

Murashige and Skoog medium

PN :

Net photosynthetic rate

RWC:

Relative water content

SOD:

Super oxide dismutase

TDZ:

thidiazuron

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Acknowledgements

The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding to this Research group No. (RGP-066).

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Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Iram Siddique & Najat Abdullah Wahab Bukhari

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  1. Iram Siddique
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  2. Najat Abdullah Wahab Bukhari
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Correspondence to Iram Siddique.

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Siddique, I., Bukhari, N.A.W. Synthetic seed production by encapsulating nodal segment of Capparis decidua (Forsk.), in vitro regrowth of plantlets and their physio biochemical studies. Agroforest Syst 92, 1711–1719 (2018). https://doi.org/10.1007/s10457-017-0120-7

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  • DOI: https://doi.org/10.1007/s10457-017-0120-7

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