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Changes in photosynthetic activity, pigment composition, electrolyte leakage, lipid peroxidation, and antioxidant enzymes during ex vitro establishment of micropropagated Rauvolfia tetraphylla plantlets

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Abstract

The effects of photosynthetic photon flux density (PPFD) on antioxidant metabolism and photosynthetic properties in leaves during ex vitro establishment of micropropagated Rauvolfia tetraphylla plantlets were investigated. In vitro-propagated plantlets were acclimatized at either 50 (Low-light = LL) or 300 (High-light = HL) μmol m−2s−1 photosynthetic PPFD for 4 weeks under controlled conditions. Increases in chlorophyll (Chl) a, b and carotenoid levels were observed in plantlets acclimatized at both light intensities. At transplantation, micropropagated plantlets were not photosynthetically active, but the net photosynthetic rate increased in newly formed leaves over time during acclimatization. The observed differences in pigment contents and photosynthetic rates suggested adaptation of plantlets from heterotrophic to autotrophic mode of nutrition during acclimatization. Changes in activities of antioxidant enzymes were also observed during acclimatization. Superoxide dismutase activity increased in plantlets acclimatized at HL intensities. Likewise, changes in activity of catalase and ascorbate peroxidase were also detected. These observed changes reflected the ability of plants in developing an antioxidant enzymatic defense system aiding in survival against oxidative stress and in reducing release of free radicals.

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Acknowledgments

The authors gratefully acknowledge the Department of Science and Technology, Government of India, New Delhi for the award of a SERC Fast Track Scheme (Project No. SR/FT/L-067/2006) to MF and for providing research support under DST-FIST (2005) programme. The authors are also thankful to Professor M. Iqbal, Jamia Hamdard, New Delhi and Dr. Conor O’Reilly, UCD School of Biology and Environmental Science, University College, Dublin for their critical reading and comments on the manuscript.

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  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    M. Faisal & M. Anis

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  1. M. Faisal
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  2. M. Anis
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Correspondence to M. Faisal.

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Faisal, M., Anis, M. Changes in photosynthetic activity, pigment composition, electrolyte leakage, lipid peroxidation, and antioxidant enzymes during ex vitro establishment of micropropagated Rauvolfia tetraphylla plantlets. Plant Cell Tiss Organ Cult 99, 125–132 (2009). https://doi.org/10.1007/s11240-009-9584-0

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