Abstract
Synseeds were produced from aseptic axillary buds of mulberry variety – S54 (Morus indica L.) to investigate their in vitro and ex vitro conversion potential. The synseeds when cultured on Linsmaier and Skoog's basal medium supplemented with 6-benzyl amino purine (8.88 μM) and 2,3,5-tri iodo benzoic acid (2 μM), produced shoots after 21 days exhibiting 48.2±0.60 in vitro conversion response. The synseeds ex␣vitro conversion response was found to be 45.5±0.76 on soilrite mix containing half strength LS nutrients after 21 days of sowing. Further, synseeds stored at 4 °C for 1–4 months resulted in maximum conversion response under both in vitro and ex vitro conditions, followed by development into complete plantlets without any significant loss of conversion potential. Plants regenerated from the synseeds of axillary bud were hardened, acclimatized and established in soil with varying survival frequency.
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Abbreviations
- BAP:
-
6-benzyl amino purine
- LSBM:
-
Linsmaier and Skoog's basal medium
- Synseed (s):
-
synthetic seed (s)
- TIBA:
-
2,3,5-tri iodo benzoic acid
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Acknowledgement
Financial assistance provided by Council of Scientific and Industrial Research (CSIR), New Delhi, to one of the authors R. Kavyashree, is gratefully acknowledged.
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R., K., M.C., G. & H.M., R. Propagation of mulberry variety – S54 by synseeds of axillary bud. Plant Cell Tiss Organ Cult 84, 245–249 (2006). https://doi.org/10.1007/s11240-005-9022-x
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DOI: https://doi.org/10.1007/s11240-005-9022-x