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Direct plant regeneration from encapsulated nodal segments of Vitex negundo

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na2-alginate with 100 mM CaCl2 has been found to be optimum concentration for the production of uniform synthetic seed. For germination, the synseeds were cultured on Murashige and Skoog (MS) basal medium supplemented with kinetin (KIN) and α-naphthalene acetic acid (NAA) either singly or in various combinations. MS medium containing 2.5 μM KIN in combination with 1.0 μM NAA was found to be the optimum for maximum (92.6 ± 3.71 %) plantlet conversion frequency. Well developed regenerated plantlets were hardened, acclimatized and established in field, where they grew well without any detectable variation.

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Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KIN:

kinetin

MS:

Murashige and Skoog medium

NAA:

α-naphthalene acetic acid

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Acknowledgements

The award of a SRF and Young Scientist (SR/FT/LS-014/2009) to N.A. by the Council of Scientific and Industrial Research (CSIR) and Department of Science and Technology (DST), Government of India, New Delhi, is greatly acknowledged. Research support from The Department of Science and Technology (Govt. of India) New Delhi under the DST- FIST (2005) and UGC-SAP (2009) Programme, is also acknowledged.

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Authors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India

    N. Ahmad & M. Anis

  2. College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia

    M. Anis

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  1. N. Ahmad
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  2. M. Anis
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Correspondence to M. Anis.

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Ahmad, N., Anis, M. Direct plant regeneration from encapsulated nodal segments of Vitex negundo . Biol Plant 54, 748–752 (2010). https://doi.org/10.1007/s10535-010-0134-8

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  • DOI: https://doi.org/10.1007/s10535-010-0134-8

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