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Three highly similar formate dehydrogenase genes located in the vicinity of the B4 resistance gene cluster are differentially expressed under biotic and abiotic stresses in Phaseolus vulgaris

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Abstract

In higher plants, formate dehydrogenase (FDH, EC1.2.1.2.) catalyzes the NAD-linked oxidation of formate to CO2, and FDH transcript accumulation has been reported after various abiotic stresses. By sequencing a Phaseolus vulgaris BAC clone encompassing a CC-NBS-LRR gene rich region of the B4 resistance gene cluster, we identified three FDH-encoding genes. FDH is present as a single copy gene in the Arabidopsis thaliana genome, and public database searches confirm that FDH is a low copy gene in plant genomes, since only 33 FDH homologs were identified from 27 plant species. Three independent prediction programs (Predotar, TargetP and Mitoprot) used on this large subset of 33 plant FDHs, revealed that mitochondrial localization of FDH might be the rule in higher plants. A phylogenetic analysis suggests a scenario of local FDH gene duplication in an ancestor of the Phaseoleae followed by another more recent duplication event after bean/soybean divergence. The expression levels of two common bean FDH genes under different treatments were investigated by quantitative RT-PCR analysis. FDH genes are differentially up-regulated after biotic and abiotic stresses (infection with the fungus Colletotrichum lindemuthianum, and dark treatment, respectively). The present study provides the first report of FDH transcript accumulation after biotic stress, suggesting the involvement of FDH in the pathogen resistance process.

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Abbreviations

BAC:

Bacterial artificial chromosome

RT-PCR:

Reverse transcription polymerase chain reaction

FDH:

Formate dehydrogenase

Mya:

Million years ago

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Acknowledgments

The authors are grateful to Steven B. Cannon for critical reading of the manuscript. They also thank Mathilde Langlois-Meurinne and Claire Gachon for helpful discussions and Jim Kami and Paul Gepts for providing us the Phaseolus vulgaris BAT93 FY-N24 BAC clone. Special thanks to Georgina Hernández for sharing ESTs data prior to publication. The research was supported by INRA-DGAP, CNRS and the French Ministère de la Recherche.

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Authors and Affiliations

  1. Institut de Biotechnologie des Plantes, UMR-CNRS 8618, bât. 630, Université Paris-Sud, 91405, Orsay, France

    Perrine David, Catherine Colas des Francs-Small, Mireille Sévignac, Vincent Thareau, Catherine Macadré, Thierry Langin & Valérie Geffroy

  2. ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Catherine Colas des Francs-Small

  3. Unité Mixte de Recherche de Génétique Végétale, Institut National de la Recherche Agronomique, 91190, Gif-sur-Yvette, France

    Valérie Geffroy

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  1. Perrine David
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Correspondence to Valérie Geffroy.

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Communicated by H. T. Nguyen.

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Supplementary material 1 (DOCX 16 kb)

Specificity of primer pairs used in RT-qPCR experiments (PPT 286 kb)

122_2010_1293_MOESM3_ESM.ppt

Multiple alignment performed on 36 FDH deduced amino acid sequences using the Clustal X software and edited in GENEDOC for manual adjustments. Details concerning the origin of the 36 FDH sequences are given in Supplementary Table 1. Peptide signal, as defined on the potato Z21493 sequence (Colas des Francs et al. 2003), is indicated. The multiple alignment used for phylogenetic analyses is based on the region between the two black arrows (PPT 233 kb)

122_2010_1293_MOESM4_ESM.ppt

Analysis of the flanking sequences of the three FDH genes. Hatched areas designate regions presenting > 38% nucleotide identity. Size in base pair of each considered region is indicated in brackets (PPT 165 kb)

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David, P., Colas des Francs-Small, C., Sévignac, M. et al. Three highly similar formate dehydrogenase genes located in the vicinity of the B4 resistance gene cluster are differentially expressed under biotic and abiotic stresses in Phaseolus vulgaris . Theor Appl Genet 121, 87–103 (2010). https://doi.org/10.1007/s00122-010-1293-x

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