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Resistance to Colletotrichum lindemuthianum in Phaseolus vulgaris: a case study for mapping two independent genes

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Abstract

Anthracnose, caused by the hemibiotrophic fungal pathogen Colletotrichum lindemuthianum is a devastating disease of common bean. Resistant cultivars are economical means for defense against this pathogen. In the present study, we mapped resistance specificities against 7 C. lindemuthianum strains of various geographical origins revealing differential reactions on BAT93 and JaloEEP558, two parents of a recombinant inbred lines (RILs) population, of Meso-american and Andean origin, respectively. Six strains revealed the segregation of two independent resistance genes. A specific numerical code calculating the LOD score in the case of two independent segregating genes (i.e. genes with duplicate effects) in a RILs population was developed in order to provide a recombination value (r) between each of the two resistance genes and the tested marker. We mapped two closely linked Andean resistance genes (Co-x, Co-w) at the end of linkage group (LG) B1 and mapped one Meso-american resistance genes (Co-u) at the end of LG B2. We also confirmed the complexity of the previously identified B4 resistance gene cluster, because four of the seven tested strains revealed a resistance specificity near Co-y from JaloEEP558 and two strains identified a resistance specificity near Co-9 from BAT93. Resistance genes found within the same cluster confer resistance to different strains of a single pathogen such as the two anthracnose specificities Co-x and Co-w clustered at the end of LG B1. Clustering of resistance specificities to multiple pathogens such as fungi (Co-u) and viruses (I) was also observed at the end of LG B2.

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Acknowledgment

We thank Guy Fouilloux for helpful discussions. The research was supported by INRA-DGAP, CNRS and Ministère de la recherche.

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Authors and Affiliations

  1. Institut de Biotechnologie des Plantes (IBP), INRA, Université Paris-Sud, UMR-CNRS 8618, bât. 630, 91405, Orsay Cedex, France

    Valérie Geffroy, Mireille Sévignac, Michel Dron & Thierry Langin

  2. Ecole Polytechnique, LadHyX, UMR-CNRS 7646, 91128, Palaiseau Cedex, France

    Paul Billant

Authors
  1. Valérie Geffroy
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  2. Mireille Sévignac
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  3. Paul Billant
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  4. Michel Dron
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  5. Thierry Langin
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Correspondence to Valérie Geffroy.

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Communicated by D. Mather.

Appendix

Appendix

In the case of two complementary resistance genes (case of complementary epistasis), the same procedure as described previously for two independent genes can be used. The frequency of the different genotypic classes are given in Table 7, which is identical as in Table 4 except that the lines MM/mm and the columns Resistant/Susceptible are inverted, i.e. (x, y, z, t) change to (t, z, y, x). The probability of linkage is:

$$ P(r) = \frac{{{\left( {a + b + c + d} \right)}!}} {{a!b!c!d!}}t^{a} z^{b} y^{c} x^{d} $$

The value of r maximizing the LOD score is given by substituting (d, c, b, a) for (a, b, c, d) in Eq. (2)

Table 7 Frequencies of phenotypic classes, when two complementary resistance genes are present in parent A and that there is a recombination rate r between G1 and marker M
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Geffroy, V., Sévignac, M., Billant, P. et al. Resistance to Colletotrichum lindemuthianum in Phaseolus vulgaris: a case study for mapping two independent genes. Theor Appl Genet 116, 407–415 (2008). https://doi.org/10.1007/s00122-007-0678-y

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