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The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes

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Abstract

The broadest based resistance to anthracnose of common bean (Phaseolus vulgaris L.) is conferred by the Co-4 locus. We sequenced a bacterial artificial chromosome clone harboring part of the Co-4 locus of the bean genotype Sprite and assembled a single contig of 106.5 kb for functional annotation. This region contained five copies of the COK-4 gene that encodes for a serine threonine kinase protein previously mapped to the Co-4 locus and 19 novel genes with no similarity to any previously identified genes of common bean. Several putative genes of the Co-4 locus seemed to be expressed as they matched perfectly with bean expressed sequence tags. The expression of the COK-4 genes was assessed by reverse transcription (RT)-PCR, and a single 850-bp cDNA fragment was sequenced and compared with the genomic sequences of the COK-4 homologs. Although the COK-4 cDNA was isolated from a different bean cultivar, it showed high similarity (95%) to the exons of genes BA17 and BA21, suggesting that they were expressed. In a phylogenetic tree including all currently available Pto-like sequences from Phaseolus species, the COK-4 homologs formed a single cluster with the Pto gene, whereas two sequences from P. coccineus and all sequences of P. vulgaris formed two closely related clusters. The Co-4 locus was physically mapped to the short arm of bean chromosome 3, which corresponds to linkage group B8. This study represents a first step in gaining an understanding of the genomic organization of an anthracnose resistance locus of common bean and provides molecular data for comparative analysis with other plant species.

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Acknowledgements

We thank Dr. Sally MacKenzie (University of Nebraska) for screening her BAC library with the SAS13 marker and kindly donating the four homologous BAC clones, Dr. Carlos Eduardo Vallejos (University of Florida) for providing seeds of the cultivar Calima and the RFLP clones from the University of Florida linkage map, and Dr. Dieter Schweizer (University of Vienna) for supporting the participation of A.P.-H in this project. We thank Dr. Claudia B. Monteiro-Vitorello for assisting with the assembly of the DNA contig, Dr. Jorge L.M. Rodrigues and Dr. Sheng Yang He for helpful suggestions and critical review of the manuscript, and Daniela Truffi and Maria Cristina R. Rangel for assisting with DNA sequencing. M.M. acknowledges financial support for this project from FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (Grant 00/09049-2). Research at L.E.A. Camargo’s lab is supported by funds from FAPESP (Grant 00/09059-8).

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  1. M. Melotto

    Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 206 Plant Biology Building, East Lansing, MI, 48824, USA

Authors and Affiliations

  1. Departamento de Fitopatologia, Laboratório de Genética Molecular, ESALQ, Universidade de São Paulo, Piracicaba, SP, C.P. 9, 13418-900, Brazil

    M. Melotto, M. F. Coelho & L. E. A. Camargo

  2. Department of Cell Biology and Genetics, Institute of Botany, University of Vienna, Rennweg 14, Vienna, 1030, Austria

    A. Pedrosa-Harand

  3. Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI, 48824, USA

    J. D. Kelly

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Correspondence to M. Melotto.

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Melotto, M., Coelho, M.F., Pedrosa-Harand, A. et al. The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes. Theor Appl Genet 109, 690–699 (2004). https://doi.org/10.1007/s00122-004-1697-6

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