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Cell surface engineering of yeast for applications in white biotechnology

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Abstract

Cell surface engineering is a promising strategy for the molecular breeding of whole-cell biocatalysts. By using this strategy, yeasts can be constructed by the cell surface display of functional proteins; these yeasts are referred to as arming yeasts. Because reactions using arming yeasts as whole-cell biocatalysts occur on the cell surface, materials that cannot enter the cell can be used as reaction substrates. Numerous arming yeasts have therefore been constructed for a wide range of uses such as biofuel production, synthesis of valuable chemicals, adsorption or degradation of environmental pollutants, recovery of rare metal ions, and biosensors. Here, we review the science of yeast cell surface modification as well as current applications and future opportunities.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Kouichi Kuroda & Mitsuyoshi Ueda

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  1. Kouichi Kuroda
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  2. Mitsuyoshi Ueda
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Correspondence to Mitsuyoshi Ueda.

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Kuroda, K., Ueda, M. Cell surface engineering of yeast for applications in white biotechnology. Biotechnol Lett 33, 1–9 (2011). https://doi.org/10.1007/s10529-010-0403-9

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