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A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering.

Methods:

We evaluated the effect of n-HA and n-SiO2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay.

Results:

All the samples demonstrated bead-less morphologies with an average diameter in the range of 190–260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO2. While the hydrophilicity of n-SiO2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO2.

Conclusion:

Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

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Acknowledgements

The authors were financially supported by a grant (NO: 94/104) from Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

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Authors and Affiliations

  1. Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran

    Vahideh Raeisdasteh Hokmabad & Ali Ramazani

  2. Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733, Iran

    Soodabeh Davaran & Roya Salehi

  3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 51666-14733, Iran

    Marziyeh Aghazadeh & Effat Alizadeh

  4. Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 51666-14733, Iran

    Marziyeh Aghazadeh

  5. Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733, Iran

    Effat Alizadeh

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  1. Vahideh Raeisdasteh Hokmabad
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  2. Soodabeh Davaran
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  3. Marziyeh Aghazadeh
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  4. Effat Alizadeh
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  5. Roya Salehi
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  6. Ali Ramazani
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Correspondence to Roya Salehi or Ali Ramazani.

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All experimental protocols were approved by the Ethics Committee of Tabriz University of Medical Sciences (TUMS) which was in compliance with Helsinki declaration, and all participants signed the informed consent (Approval No. 56/6925). There are no animal experiments carried out for this article.

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Hokmabad, V.R., Davaran, S., Aghazadeh, M. et al. A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering. Tissue Eng Regen Med 15, 735–750 (2018). https://doi.org/10.1007/s13770-018-0140-z

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