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3D scaffold with PCL combined biomedical ceramic materials for bone tissue regeneration

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Abstract

Three-dimensional porous biodegradable polymer scaffolds have been widely used for tissue engineering of bone repair or regeneration. The primary function of scaffolds is to provide structure support for the cells adhesion and proliferation. This study selects the Poly-ɛ-caprolactone (PCL) as material, NaCl mixed with hydroxyapatite (HA) or nano-aluminum oxide (nAl2O3) for porous scaffold. This study uses the solvent casting/particulate leaching method to fabricate the porous scaffold. The authors discuss the compression mechanical properties, physical properties (porosity, moisture content, contact angle) of a pure PCL, PCL/mHA, PCL/nHA and PCL/nAl2O3 scaffolds. In vitro cell culture is used for osteoblast cell (MG63) and the microculture tetrazolium test (MTT) is undertaken in the scaffold. The scaffolds are implanted to the femur of rats and histological examination is attempted after 2 weeks. The experimental results indicate that HA and nAl2O3 can improve the hydrophilic property. In conclusion, the PCL/nHA scaffold exhibits splendid in vivo biocompatibility and osteogenesis.

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Authors and Affiliations

  1. Department of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan, R.O.C.

    Ming-Jyh Chern & Jia-Hsiang Hung

  2. Department of Physiology, School of Medicine, College of Medicine, Taipei Medicine University, Taipei, 11031, Taiwan, R.O.C

    Liang-Yo Yang

  3. Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei, 11031, Taiwan, R.O.C

    Liang-Yo Yang

  4. Neuroscience Research Center, Taipei Medical University Hospital, Taipei, 11031, Taiwan, R.O.C

    Liang-Yo Yang

  5. Biomedical Mass Imaging Research Center, Taipei Medical University, Taipei, 11031, Taiwan, R.O.C

    Liang-Yo Yang

  6. School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, 11031, Taiwan, R.O.C

    Yung-Kang Shen

Authors
  1. Ming-Jyh Chern
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  2. Liang-Yo Yang
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  3. Yung-Kang Shen
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  4. Jia-Hsiang Hung
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Correspondence to Yung-Kang Shen.

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Chern, MJ., Yang, LY., Shen, YK. et al. 3D scaffold with PCL combined biomedical ceramic materials for bone tissue regeneration. Int. J. Precis. Eng. Manuf. 14, 2201–2207 (2013). https://doi.org/10.1007/s12541-013-0298-1

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  • DOI: https://doi.org/10.1007/s12541-013-0298-1

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