Skip to main content
SpringerLink
Log in

In vitro selection and identification of a cold-tolerant variant in pineapple (Ananas comosus)

  • Research Report
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

Cold stress is an important factor limiting the growth and distribution of pineapple. Breeding cold-resistant germplasm is an effective way to cope with this problem. In vitro selection for the somaclonal variation using different selection agents has been used for crop improvement in stress tolerance. In this study, the pineapple cold-tolerant variant was developed through in vitro cold selection of somaclonal variations. Low temperature was used as the selected agent, and the extreme lethal condition for the in vitro pineapple cultures was determined to be 0 °C for 72 h. The morphology changes of the in vitro cultures during the cold selection were observed and analyzed. The cold-tolerant variant line was finally obtained through three consecutive selections with cold shock treatments, based on the established high-efficiency culture system for pineapple embryogenic calli. The genetic variations at the molecular level in the cold-tolerant variant were verified by ISSR analysis. The significantly improved cold tolerance in our selected variant was mainly reflected by the higher survival rate, increased proline content, and elevated SOD activity under cold stress compared to these qualities in the control plants. This study demonstrated the feasibility of in vitro selection for cold tolerance in pineapple. The cold-tolerant variant could be valuable for future pineapple breeding programs and for cold tolerance research.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Anis M, Ahmad N (2016) Plant tissue culture: propagation, conservation and crop improvement. Springer, Singapore

    Book  Google Scholar 

  • Anwar A, Kikuchi A, Watanabe KN (2010) Assessment of somaclonal variation for salinity tolerance in sweet potato regenerated plants. Afr J Biotechnol 9:7256–7265

    CAS  Google Scholar 

  • Ashrafzadeh S, Leung DMW (2015) In vitro breeding of heavy metal-resistant plants: a review. Hortic Environ Biote 56:131–136

    Article  CAS  Google Scholar 

  • Bairu MW, Aremu AO, Van Staden J (2010) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173

    Article  Google Scholar 

  • Baker CJ, Mock NM (1994) An improved method for monitoring cell death in cell suspension and leaf disc assays using evans blue. Plant Cell Tissue Organ Cult 39:7–12

    Article  Google Scholar 

  • Bartholomew DP, Paull RE, Rohrbach KG (2002) The pineapple: botany, production, and uses. CABI

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bertin P, Bouharmont J (1997) Use of somaclonal variation and in vitro selection for chilling tolerance improvement in rice. Euphytica 96:135–142

    Article  Google Scholar 

  • Caboni E, Anselmi S, Donato E, Manes F (2003) In vitro selection of Actinidia deliciosa clones tolerance to NaCl and their molecular and in vivo ecophysiological characterisation. Acta Hortic 618:77–83

    Article  CAS  Google Scholar 

  • Carloni E, Tommasino E, Colomba EL, Ribotta A, Quiroga M, Griffa S, Grunberg K (2017) In vitro selection and characterization of buffelgrass somaclones with different responses to water stress. Plant Cell Tissue Organ Cult 130:265–277

    Article  Google Scholar 

  • Chen L, Xiang H, Miao Y, Zhang L, Guo Z, Zhao X, Lin J, Li T (2014a) An overview of cold resistance in plants. J Argon Crop Sci 200:237–245

    Article  CAS  Google Scholar 

  • Chen Y, Jiang J, Chang Q, Gu C, Song A, Chen S, Dong B, Chen F (2014b) Cold acclimation induces freezing tolerance via antioxidative enzymes, proline metabolism and gene expression changes in two chrysanthemum species. Mol Biol Rep 41:815–822

    Article  CAS  PubMed  Google Scholar 

  • Chen C, Zhang Y, Xu Z, Luan A, Mao Q, Feng J, Xie T, Gong X, Wang X, Chen H, He Y (2016) Transcriptome profiling of the pineapple under low temperature to facilitate its breeding for cold tolerance. PLoS ONE 11:e0163315

    Article  PubMed  PubMed Central  Google Scholar 

  • Dhurve L, Ajith Kumar K, Bhaskar J, Sobhana A, Francies RM, Mathew D (2021) Wide variability among the ‘Mauritius’ somaclones demonstrates somaclonal variation as a promising improvement strategy in pineapple (Ananas comosus L.). Plant Cell Tissue Organ Cult 145:701–705

    Article  CAS  Google Scholar 

  • Dix PJ (1993) The role of mutant cell lines in studies on environmental stress tolerance: an assessment. Plant J 3:309–313

    Article  CAS  Google Scholar 

  • Dong HL, Lee CB (2000) Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci 159:75

    Article  Google Scholar 

  • Dörffling K, Dörffling H, Lesselich G (1993) In vitro-Selection and regeneration of hydroxyproline-resistant lines of winter wheat with increased proline content and increased frost tolerance. J Plant Physiol 142:222–225

    Article  Google Scholar 

  • Duncan DR, Widholm JM (1987) Proline accumulation and its implication in cold tolerance of regenerable maize callus. Plant Physiol 83:703

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Efferth T (2019) Biotechnology applications of plant callus cultures. Engineering 5:50–59

    Article  CAS  Google Scholar 

  • Frascaroli E (2018) Breeding cold-tolerant crops. In: Wani SH (ed) Cold tolerance in plants, 1st edn. Springer, Cham, pp 159–177

    Chapter  Google Scholar 

  • Ge H, Li Y, Fu H, Long G, Luo L, Li R, Deng Z (2015) Production of sweet orange somaclones tolerant to citrus canker disease by in vitro mutagenesis with EMS. Plant Cell Tissue Organ Cult 123:29–38

    Article  CAS  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in Higher Plants Plant Physiol 59:309–314

    CAS  PubMed  Google Scholar 

  • Gosal SS, Kang MS (2012) Plant tissue culture and genetic transformation for crop improvement. Wiley, Hoboken

    Book  Google Scholar 

  • Halim NAA, Tan BC, Midin MR, Madon M, Khalid N, Yaacob JS (2018) Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2. Plant Cell Tissue Organ Cult 133:123–135

    Article  Google Scholar 

  • Hammerschlag FA (2000) Resistant responses of peach somaclone 122–1 to Xanthomonas campestris pv. pruni and to Pseudomonas syringae pv. syringae. HortScience 35:141–143

    Article  Google Scholar 

  • He YH, Luo J, Hu ZY, Wang RX, Gao AP, Zhao CX, Yang XH, Ye ZX, Wang ZH (2008) Classification and organogenesis of non-embryogenic callus from Ananas comosus. J Fruit Sci 25:65–68

    Google Scholar 

  • He YH, Fang SQ, Ma J, Hu ZY, Lu M, Peng B (2010) Histocytology observation on the somatic embryogenesis in Ananas comosus callus. Acta Horticult Sin 37:689–696

    CAS  Google Scholar 

  • He YH, Fang SQ, Hu ZY, Ma J, Luo J, Wu CH, Cao L, Lu M, Chen CJ (2012) Morphological and Anatomical analysis of pineapple somatic embryogenesis. Acta Horticult Sin 39:57–63

    CAS  Google Scholar 

  • Hwang SC, Ko WH (2004) Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan. Plant Dis 88:580–588

    Article  PubMed  Google Scholar 

  • Jain SM, Brar DS, Ahloowalia BSE (2013) Somaclonal variation and induced mutations in crop improvement, vol 32. Springer, New York

    Google Scholar 

  • Koç İ, Akdemir H, Onay A, Çiftçi YÖ (2014) Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets. Acta Physiol Plant 36:2373–2384

    Article  Google Scholar 

  • Kohpaii FN, Farahani F, Noormohammadi Z (2017) Somaclonal variation in the in vitro regenerated pineapple (Ananas comosus): investigation of the cellular characteristics biochemical specificities and ISSR markers. Phytol Balc 23:73–83

    Google Scholar 

  • Krishna H, Alizadeh M, Singh D, Singh U, Chauhan N, Eftekhari M, Sadh RK (2016) Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech 6:1–18

    Article  Google Scholar 

  • Kuksova VB, Piven NM, Gleba YY (1997) Plant cell variation and in vitro induced mutagenesis in grapevine. Plant Cell Tissue Organ Cult 49:17–27

    Article  CAS  Google Scholar 

  • Li R, Qu R, Bruneau AH, Livingston DP (2010) Selection for freezing tolerance in St. Augustinegrass through somaclonal variation and germplasm evaluation. Plant Breed 129:417–421

    Google Scholar 

  • Liu J, Yang Z, Li W, Yu J, Huang B (2013) Improving cold tolerance through in vitro selection for somaclonal variations in Seashore paspalum. J Am Soc Hortic Sci 138:452–460

    Article  CAS  Google Scholar 

  • Liu MY, Sun J, Wang KY, Liu D, Li ZY, Zhang J (2014) Spermidine enhances waterlogging tolerance via regulation of antioxidant defence, heat shock protein expression and plasma membrane H+-ATPase activity in Zea mays. J Argon Crop Sci 200:199–211

    Article  CAS  Google Scholar 

  • Lobo MG, Paull RE (2017) Handbook of pineapple technology: postharvest science, processing and nutrition. Wiley, Hoboken

    Book  Google Scholar 

  • Lu XH, Sun DQ, Ye CH, Li YZ, Sun GM (2010) Growth, physiological characteristics and evaluation of cold tolerance of pineapple seedlings under low temperature stress. Acta Bot Boreali Occident Sin 30:2054–2060

    CAS  Google Scholar 

  • Luan A, He Y, Xie T, Chen CJ, Mao Q, Wang X, Li C, Ding Y, Lin W, Liu C, Xia J, He J (2019) Identification of an embryonic cell-specific region within the pineapple SERK1 promoter. Genes 10:883

    Article  CAS  PubMed Central  Google Scholar 

  • Ma J, He Y, Wu C, Liu H, Hu Z, Sun G (2011) Cloning and Molecular characterization of a SERK gene transcriptionally induced during somatic embryogenesis in Ananas comosus cv. Shenwan. Plant Mol Biol Rep 30:195–203

    Article  CAS  Google Scholar 

  • Maleki M, Ghorbanpour M, Nikabadi S, Wani SH (2019) In vitro screening of crop plants for abiotic stress tolerance. In: Wani S (ed) recent approaches in omics for plant resilience to climate change. Springer, Cham, pp 75–91

    Chapter  Google Scholar 

  • Montero-Calderón M, Cerdas-Araya MDM (2012) Tropical and subtropical fruits: postharvest physiology, processing and packaging. Wiley, Hoboken

    Google Scholar 

  • Nacheva LR, Gercheva PS, Andonova MY, Panayotova DV, Dzhuvinov VT (2014) Somaclonal variation: a useful tool to improve disease resistance of pear rootstock ‘Old Home 9 Farmingdale’(OHF 333) (Pyrus communis L.). Acta Hortic 1056:253–258

    Article  Google Scholar 

  • Ogata T, Yamanaka S, Shoda M, Urasaki N, Yamamoto T (2016) Current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in Japan: pineapple, mango, and papaya. Breed Sci 66:69–81

    Article  PubMed  PubMed Central  Google Scholar 

  • Pérez G, Mbogholi A, Sagarra F, Aragón C, González J, Isidrón M, Lorenzo JC (2011) Morphological and physiological characterization of two new pineapple somaclones derived from in vitro culture. In Vitro Cell Dev Plant 47:428–433

    Article  Google Scholar 

  • Pérez G, Yanez E, Mbogholi A, Valle B, Sagarra F, Yabor L, Aragón C, González J, Isidrón M, Lorenzo JC (2012) New pineapple somaclonal variants: P3R5 and Dwarf. Am J Plant Sci 03:1–11

    Article  Google Scholar 

  • Py C, Lacoeuilhe JJ, Teisson C (1987) The pineapple cultivation and uses. G-P Maisonneuve & Larose, Pairs

  • Rai MK, Kalia RK, Singh R, Gangola MP, Dhawan AK (2011) Developing stress tolerant plants through in vitro selection: an overview of the recent progress. Environ Exp Bot 71:89–98

    Article  Google Scholar 

  • Sanewski GM (2018) The history of pineapple improvement, genetics and genomics of pineapple. Springer, Cham

    Google Scholar 

  • Sengar AS, Thind KS, Kumar B, Pallavi M, Gosal SS (2009) In vitro selection at cellular level for red rot resistance in sugarcane Saccharum sp. Plant Growth Regul 58:201–209

    Article  CAS  Google Scholar 

  • Shi H, Huang R, Liu Y, Chen X, Lu S, Guo Z (2020) Identification of a cold tolerant mutant in seashore paspalum (Paspalum vaginatum). Plant Cell Tiss Organ Cult 140:379–387

    Article  CAS  Google Scholar 

  • Siddique I (2020) Propagation and genetic manipulation of plants. Springer, Singapore

    Google Scholar 

  • Silva RLD, Ferreira CF, Ledo CADS, Souza EHD, Silva PHD, Souza FVD (2016) Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation. Plant Cell Tiss Organ Cult 127:123–133

    Article  Google Scholar 

  • Smith MK, Botella JR (2020) Ananas comosus pineapple. In: Richard EL (ed) Biotechnology of fruit and nut crops, 2nd edn. CABI, Boston, pp 118–130

    Chapter  Google Scholar 

  • Sripaoraya S, Marchant R, Power JB, Davey MR (2003) Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cell Dev Plant 39:450–454

    Article  Google Scholar 

  • Steponkus PL, Lanphear FO (1967) Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol 42:1423

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tal M (1994) In vitro selection for salt tolerance in crop plants: Theoretical and practical considerations. In Vitro Plant 30:175–180

    Article  Google Scholar 

  • Tricker P, Trewin HO, Clarkson G, Eensalu E, Tallis M, Colella A, Doncaster C, Sabatti M, Taylor G (2005) Stomatal conductance and not stomatal density determines the long-term reduction in leaf transpiration of poplar in elevated CO2. Oecologia 143:652–660

    Article  PubMed  Google Scholar 

  • Turk H, Erdal S, Genisel M, Atici O, Demir Y, Yanmis D (2014) The regulatory effect of melatonin on physiological, biochemical and molecular parameters in cold-stressed wheat seedlings. Plant Growth Regul 74:139–152

    Article  CAS  Google Scholar 

  • Vanijajiva O (2012) Assessment of genetic diversity and relationships in pineapple cultivars from Thailand using ISSR marker. Int J Agric Technol 8:1829–1838

    CAS  Google Scholar 

  • Verma D, Ansari MW, Agrawal GK, Rakwal R, Shukla A, Tuteja N (2013) In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance. Plant Signal Behav 8:e23519

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang SM, Lan H, Jia HH, Xie KD, Wu XM, Chen CL, Guo WW (2016) Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck). Sci Hortic-Amsterdam 207:233–239

    Article  Google Scholar 

  • Whitehouse AB, Johnson AW, Passey AJ, McLeary KJ, Simpson DW (2014) Serenity’: a paler skin-coloured somaclonal variant of the short-day cultivar ‘Florence. Acta Hortic 1049:819–821

    Article  Google Scholar 

Download references

Funding

This work was funded by National Natural Science Foundation of China (31801831), National Key Research and Development Project (2019YFD1000505, 2018YFD10005000).

Author information

Authors and Affiliations

  1. Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, Guangdong, China

    Yafen Zhang, Zhiqiang Xu, Tao Xie, Wei Zhang, Yehua He & Chaoyang Liu

  2. College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, China

    Yafen Zhang, Zhiqiang Xu, Tao Xie, Wei Zhang, Yehua He & Chaoyang Liu

Authors
  1. Yafen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhiqiang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tao Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yehua He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chaoyang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

CYL and YHH conceived and designed the experiments; YFZ, ZQX, WZ performed the experiments; TX and YFZ analyzed the data; ZQX and WZ contributed reagents and materials; CYL and YHH wrote the paper.

Corresponding authors

Correspondence to Yehua He or Chaoyang Liu.

Ethics declarations

Conflict of interest

There is no conflict of interest between the authors.

Additional information

Communicated by Ali Sarkhosh.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Xu, Z., Xie, T. et al. In vitro selection and identification of a cold-tolerant variant in pineapple (Ananas comosus). Hortic. Environ. Biotechnol. 63, 275–286 (2022). https://doi.org/10.1007/s13580-021-00396-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-021-00396-1

Keywords

Search

Navigation