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The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis ‘Spring Dancer’

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  • Cultivation Physiology
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Abstract

In the present study, the effect of light quality on endoreduplication and growth in Phalaenopsis ‘Spring Dancer’ plantlets was studied. The response of protocorm-like body (PLB)-derived plantlets subjected to monochromatic red (R60), blue (B60), and various combinations of both lights was investigated. Flow cytometry was used to investigate the effect of light on endocycle and growth, cell division, and endopolyploidy levels. In addition, the activities of stress-related enzymes such as catalase (CAT) and peroxidase (POD) were analyzed from leaves and roots. After 8 weeks, the leaf area of plants grown under monochromatic R60 and B60 light was found to be higher than that of plants grown under other wavelengths of light, except the control plants (fluorescent light). These results revealed monochrome blue (B60) light increased the ratio of endoreduplicated cells (4C–8C). CAT activity was highest in leaves grown under R60; however, the oxidized phenol concentration in the culture medium was lowest under R60 while it was the highest under B60 and fluorescent light (F). This indicates that plantlets were less stressed under R60 than B60 or F. The results of this study reveal that stress induced by monochromatic light stimulates endopolyploidy in leaves, which may subsequently increase Phalaenopsis leaf size.

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References

  • Barow M (2006) Endopolyploidy in seed plants. BioEssays 28:271–281

    Article  CAS  PubMed  Google Scholar 

  • Boudolf V, Vlieghe K, Beemster GT, Magyar Z, Acosta JA, Maes S, Van Der Schueren E, Inzé D, De Veylder L (2004) The plant-specific cyclin-dependent kinase CDKB1; 1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis. Plant Cell 16:2683–2692

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bourget CM (2008) An introduction to light-emitting diodes. Hort Sci 43:1944–1946

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Breuer C, Ishida T, Sugimoto K (2010) Developmental control of endocycles and cell growth in plants. Curr Opin Plant Biol 13:654–660

    Article  CAS  PubMed  Google Scholar 

  • Castellano MM, del Pozo JC, Ramirez-Parra E, Brown S, Gutierrez C (2001) Expression and stability of Arabidopsis CDC6 are associated with endoreplication. Plant Cell 13:2671

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cookson SJ, Radziejwoski A, Granier C (2006) Cell and leaf size plasticity in Arabidopsis: What is the role of endoreduplication? Plant, Cell Environ 29:1273–1283

    Article  Google Scholar 

  • Cosgrove D (1981) Rapid suppression of growth by blue light. Plant Physiol 67:584–590

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • De Schutter K, Joubes J, Cools T, Verkest A, Corellou F, Babiychuk E, Van Der Schueren E, Beeckman T, Kushnir S, Inze D, De Veylder L (2007) Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint. Plant Cell 19:211–225

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dougher T, Bugbee B (2004) Long-term blue light effects on the histology of lettuce and soybean leaves and stems. J Am Soc Hortic Sci 129:467–472

    Google Scholar 

  • Folin O, Ciocalteu R (1927) On tyrosine and tryptophane determination in proteins. J Biol Chem 73:627–650

    CAS  Google Scholar 

  • Francis D (2007) The plant cell cycle: 15 years on. New Phytol 174:261–278

    Article  CAS  PubMed  Google Scholar 

  • Gendreau E, Orbovic V, Hofte H, Traas J (1999) Gibberellin and ethylene control endoreduplication levels in the Arabidopsis thaliana hypocotyls. Planta 209:513–516

    CAS  PubMed  Google Scholar 

  • Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets. J Plant Biol 43:247–250

    Article  Google Scholar 

  • Halliwell B (2008) Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies? Arch Biochem Biophys 476:107–112

    Article  CAS  PubMed  Google Scholar 

  • Heo JW, Lee CW, Paek KW (2006) Influence of mixed LED radiation on the growth of annual plants. J Plant Biol 49:286–290

    Article  Google Scholar 

  • Huang L, Yang S, Zhang S, Liu M, Lai J, Qi Y, Shi S, Wang J, Wang Y, Xie Q, Yang C (2009) The Arabidopsis SUMO E3 Ligase AtMMS21, a homologue ofNSE2/MMS21, regulates cell proliferation in the root. Plant J 60:666–678

    Article  CAS  PubMed  Google Scholar 

  • Intuwong O, Kunisaki JT, Sagawa Y (1972) Vegetative propagation of Phalaenopsis by flower stalk cuttings. Am Orchid Soc Bull 41:13–18

    Google Scholar 

  • Inze D, De Veylder L (2006) Cell cycle regulation in plant development. Ann Rev Genet 40:77–105

    Article  CAS  PubMed  Google Scholar 

  • Ishida T, Yoshimura M, Miura K, Sugomoto K (2012) MMS21/HPY2 and SIZ1, two Arabidopsis SUMO E3 ligases, have distinct functions in development. PLoS ONE 7:e46897. https://doi.org/10.1371/journal.pone.0046897

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Johkan M, Shoji K, Goto F, Hashida S, Yoshihara T (2010) Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. Hortic Sci 45:1809–1814

    Google Scholar 

  • Joubes J, Chevalier C (2000) Endoreduplication in higher plants. Plant Mol Biol 43:735–745

    Article  CAS  PubMed  Google Scholar 

  • Kasajima S, Inoue N, Mahnud R, Kato M (2008) Developmental responses of wheat cv. Norin 61 to fluence rate of green light. Plant Prod Sci 11:76–81

    Article  Google Scholar 

  • Kim HM, Kang JH, Jeong BR, Hwang SJ (2016a) Light quality and photoperiod affect growth of sowthistle (Ixeris dentata Nakai) in a closed-type plant production system. Korean J Hortic Sci Technol 34:67–76

    Article  CAS  Google Scholar 

  • Kim YJ, Kim HM, Hwang SJ (2016b) Growth and phytochemical contents of ice plant as affected by light quality in a closed-type plant production system. Korean J Hortic Sci Technol 34:878–885

    Google Scholar 

  • Kwon AR, Cui HY, Lee H, Shin H, Kang KS, Park SY (2015) Light quality affects shoot regeneration, cell division, and wood formation in elite clones of Populus euramericana. Acta Physiol Plant 37:65

    Article  Google Scholar 

  • Larkins BA, Dilkers BP, Dante RA, Coelho CM, Woo YM, Liu Y (2001) Investigating the hows and whys of DNA endoreduplication. J Exp Bot 52:183–192

    Article  CAS  PubMed  Google Scholar 

  • Lee MY, Kim SS (1994) Characteristics of six isoperoxidases from Korean radish root. Phytochemistry 35:287–290

    Article  CAS  PubMed  Google Scholar 

  • Lee MJ, Park SY, Oh MM (2015) Growth and cell division of Lettuce plants under various ratios of red to far-red light-emitting diodes. Hortic Environ Biotechnol 56:186–194

    Article  CAS  Google Scholar 

  • Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell, Tissue Organ Cult 103:155–163

    Article  Google Scholar 

  • Lin TY, Lee HC (2007) Analysis of the orchid genome size using flow cytometry. In: Chen WH, Chen HH (eds) Orchid biotechnology. World Scientific Publishing Co. Pte. Ltd, London

    Google Scholar 

  • Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM (2013) The effect of red, blue and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Sci Hortic 150:86–91

    Article  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  PubMed  Google Scholar 

  • Murdad R, Hwa KS, Seng CK, Latip MA, Aziz ZA, Ripin R (2006) High frequency multiplication of Phalaenopsis gigantean using trimmed bases protocorms technique. Sci Hortic 111:73–79

    Article  CAS  Google Scholar 

  • Nascimento L, Leal-Costa MV, Coutinho MA, Moreira Ndos S, Lage CL, Barbi Ndos S, Costa SS, Tavares ES (2013) Increased antioxidant activity and changes in phenolic profile of Kalanchoepinnata (Lamarck) person (Crassulaceae) specimens grown under supplemental blue light. Photochem Photobiol 89:391–399

    Article  CAS  PubMed  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under super bright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue Organ Cult 73:43–52

    Article  CAS  Google Scholar 

  • Park SY, Yeung EC, Paek KY (2010) Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis. Plant Biotechnol Rep 4:303–309

    Article  CAS  Google Scholar 

  • Samuoliene G, Sirtautaas R, Brazaityte A, Duchovskis P (2012) LED lighting and seasonality effects antioxidant properties of baby leaf lettuce. Food Chem 134:1494–1499

    Article  CAS  PubMed  Google Scholar 

  • Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L.), grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282

    Article  CAS  PubMed  Google Scholar 

  • Shin KS, Murthy HN, Neo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30:339–343

    Article  CAS  Google Scholar 

  • Shu CH, Tsai CC, Liao WH, Chen KY, Huang HC (2012) Effects of light quality on the accumulation of oil in a mixed culture of Chlorella sp. Saccharomyces cerevisae. J Chem Technol Biotechnol 87:601–607

    Article  CAS  Google Scholar 

  • Sugimoto-Shirasu K, Roberts K (2003) Endoreduplication and cell-size control in plants. Curr Opin Biol 6:544–553

    Article  CAS  Google Scholar 

  • Thannickal VJ, Fanburg BL (2000) Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 279:1005–1028

    Article  Google Scholar 

  • Ting CM, Lee YM, Wong CKC, Wong AS, Lung HL, Lung ML, Lo KW, Wong RNS, Mak NK (2010) 2-Methoxyestradiol induces endoreduplication through the induction of mitochondrial oxidative stress and the activation of MAPK signaling pathways. Biochem Pharmacol 79:825–841

    Article  CAS  PubMed  Google Scholar 

  • Vlieghe K, Boudolf V, Beemster GTS, Maes S, Magyar Z, Atanassova A, De Almeida Engler J, De Groodt R, Inze D, De Veylder L (2005) The DP-E2F-like gene DEL1 controls the endocycle in Arabidopsis thaliana. Curr Biol 15:59–63

    Article  CAS  PubMed  Google Scholar 

  • Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199

    Article  CAS  Google Scholar 

  • Yeung EC (1999) The use of histology in the study of plant tissue culture systems-some practical comments. In Vitro Cell Dev Biol Plant 35:137–143

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by Brain Korea (BK) plus 21 project.

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Authors and Affiliations

  1. Department of Horticultural Science, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Myung-Min Oh, Kee-Yoeup Paek & So-Young Park

  2. WellGreen Co., Chungdae-ro 1, Cheongju, 28644, Republic of Korea

    A-Reum Kwon & So-Young Park

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  1. A-Reum Kwon
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  2. Myung-Min Oh
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Correspondence to So-Young Park.

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Kwon, AR., Oh, MM., Paek, KY. et al. The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis ‘Spring Dancer’. Hortic. Environ. Biotechnol. 59, 179–188 (2018). https://doi.org/10.1007/s13580-018-0018-y

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