Abstract
Single-node, in vitro cuttings ofRehmannia glutinosa were transplanted to MS basal media and grown for 30 d. Plantlets were grown under various culture conditions: four different light qualities (red LEDs, blue LEDs, mixed LEDs, and fluorescent); with sucrose (30 mg.L-1) or without (0 mg.L-1); with air exchanges (3.5 h.-1) or without (0.1 h.L-1). Highest dry weights were obtained from plantlets under blue LEDs with 3.5h.L-1 air exchanges. Light source did not affect shoot elongation in ventilated conditions, but without ventilation, the shoots of plantlets under red LEDs were twice as long as for plantlets growing under other types of lighting. Plantlets grown without sucrose showed little difference in photosynthesis under any of the tested light qualities. In contrast, the photosynthetic rate of those in the sucrose-containing media varied according to light source.
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Literature Cited
Brown CS, Schuerger AC, Sager JC (1995) Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J Amer Soc Hort Sci120: 808–813
Bula RJ, Morrow TW, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emiting diodes as a radiation source for plants. HortScience120: 808–813
Chee R (1986) In vitro culture ofVitis: The effects of light spectrum, manganese sulfate, and potassium iodide on morphogenesis. Plant Cell Tissue Org Cult7: 121–134
Chee R, Pool RM (1989) Morphogenetic response to propagule trimming, spectral irradiance, and photoperiod of grapevine shoots recultured in vitro. J Amer Soc Hort Sci114: 350–354
Cui YY, Hahn EJ, Kozai T, Paek KY (2000) Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth ofRehmannia glutinosa plantlets in vitro. Plant Cell Tissue Org Cult62: 219–226
Dougher TA, Bugbee B (1998) Is blue light good or bad for plants? Life Supp Biosph Sci5: 129–136
Fujiwara K, Kozai T, Watanabe I (1987) Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets. J Agr Meteorol43: 21–30
Hoenecke M, Bula RJ, Tibbitts TW (1992) Importance of ‘blue’ photon levels for lettuce seedlings grown under red light-emitting diodes. HortScience27: 427–430
Kozai T, Oki H, Fujiwara K (1990) Photosynthetic characteristics of cymbidium plantlet in vitro. Plant Cell Tissue Org Cult22: 205–211
McCree KJ (1972) The action spectra, absorbance, and quantum yield of photosynthesis in crop plants. Agr Meteorol9: 191–196
Mortensen L, Stramme E (1987) Effects of light quality on some greenhouse corps. Scient Hort33: 27–36
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant15: 473–497
Ringe F, Nitsch JP (1968) Conditions leading to flower formation on excisedBegonia fragments cultured in vitro. Plant Cell Physiol9: 639–652
Sager JC, Edwards JL, Klein WH (1982) Light energy utilization efficiency of photosynthesis. Trans Amer Soc Agr Eng25: 1737–1746
Seon JH, Cui YY, Kozai T, Paek KY (2000) Influence of in vitro growth conditions on photosynthetic competence and survival rate ofRehmannia glutinosa plantlets during acclimatization period. Plant Cell Tissue Org Cult61: 135–142
Sung IK, Kitoya M, Hirano T (1998) The effects of time and intensity of supplemental blue lighting during morning twilight on growth and physiological performance of cucumber seedlings. Life Supp Biosph Sci5: 137–142
Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth ofCymbidium plantlets cultured under super bright red and blue light-emitting diodes (LEDs). J Hort Sci Biotech73: 39–44
Tennessen DJ, Singsaas EL, Sharkey TD (1994) Light-emitting diodes as a light source for photosynthesis research. Photosynth Res39: 85–92
Tripathy BC, Brown CS (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol107: 407–411
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Hahn, E.J., Kozai, T. & Paek, K.Y. Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro Growth ofRehmannia glutinosa plantlets. J. Plant Biol. 43, 247–250 (2000). https://doi.org/10.1007/BF03030425
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DOI: https://doi.org/10.1007/BF03030425