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Light quality affects shoot regeneration, cell division, and wood formation in elite clones of Populus euramericana

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Abstract

The effect of light (blue, red, and/or green) generated by light-emitting diodes on shoot regeneration was examined in selected clones, ‘I-476’ and ‘Dorskamp’, of Populus euramericana. We found that a combination of red and blue light produced the highest percentage of shoot regeneration in comparison to monochromic or fluorescent light. Further studies of light quality and in vitro growth of “Dorskamp” plantlets demonstrated the suitability of combinations of red and blue light to improve most of the characteristics studied. Flow cytometry of the leaves showed that 50 % red + 50 % blue light treatment was the most effective for inducing cell division in “Dorskamp”, whereas monochromic red and blue were more effective in “I-476”. Histological studies found that secondary xylem formation increased with the monochromic blue light treatment in the stem of “I-476”, whereas in “Dorskamp”, a combination of red and blue light (70 + 30 %, respectively) was more suitable. Our study demonstrated that combinations of red and blue light increase shoot regeneration and development by stimulation of cell division, although the response may vary among genotypes of the same species. The results of this study can contribute to mass production of the selected clones for forest establishment on reclaimed land and serve as an efficient protocol for genetic transformation of this species.

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Abbreviations

FL:

Fluorescent light

LED:

Light-emitting diode

B:

Blue LED

R:

Red LED

G:

Green LED

PPFD:

Photosynthetic photon flux density

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Acknowledgments

This study was carried out with the support of ‘Forest Science & Technology Projects (Project No. S121212L030110)’ provided by Korea Forest Service.

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    A-Reum Kwon, Hae-Yeon Cui & So-Young Park

  2. Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon, 441-847, Republic of Korea

    Hyoshin Lee & Hanna Shin

  3. Department of Forest Sciences, College of Agriculture and Life Science, Seoul National University, Seoul, 151-921, Republic of Korea

    Kyu-Suk Kang

Authors
  1. A-Reum Kwon
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  2. Hae-Yeon Cui
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  3. Hyoshin Lee
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  4. Hanna Shin
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  5. Kyu-Suk Kang
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  6. So-Young Park
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Corresponding author

Correspondence to So-Young Park.

Additional information

Communicated by U. Feller.

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Kwon, AR., Cui, HY., Lee, H. et al. Light quality affects shoot regeneration, cell division, and wood formation in elite clones of Populus euramericana . Acta Physiol Plant 37, 65 (2015). https://doi.org/10.1007/s11738-015-1812-0

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  • DOI: https://doi.org/10.1007/s11738-015-1812-0

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