Abstract
Endoreduplication is a developmental process that is unique to plants and occurs in all plants. The present study aimed to assess endoreduplication in various explant tissues and regenerated somatic embryos of Doritaenopsis. We further investigated the effects of light quality on endoreduplication and somatic embryo proliferation. To this end, we studied endoreduplication in leaves and root tips from regenerated plantlets and somatic embryos and in developing somatic embryos under 4 types of lighting conditions: red light, red + far-red light, red + blue light, and white light. We found that the degree of endoreduplication varied in different explants, and that the choice of explants used also influenced the ploidy levels of the newly regenerated somatic embryos. The DNA content of the leaf (2C–8C) was less than that of the root tip (2C–16C) and somatic embryo (2C–64C). In terms of light quality, the combination of red and far-red light produced the highest number of somatic embryos, while maintaining a low degree of endoreduplication. The data obtained indicate that this light combination stimulates somatic embryogenesis in Doritaenopsis and may exert some control on endoreduplication during cell division. These findings can be applied to achieve a reduction in somaclonal variations for the purpose of mass proliferation and genetic improvement.
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References
Arditti J, Ernst R (1993) Micropropagation of orchids. Wiley, New York, pp 467–520
Ascencio-Cabral A, Gutierrez-Pulido H, Rodríguez-Garay B, Gutiérrez-Mora A (2008) Plant regeneration of Carica papaya L. through somatic embryogenesis in response to light quality, gelling agent and phloridzin. Sci Hortic 118:155–160
Bohanec B, Jakse M (1999) Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Plant Cell Rep 18:737–742
Bouman H, De Klerk G (1997) Somaclonal variation. In: Geneve RL, Preece JE, Merkle SA (eds) Biotechnology of ornamental plants. CAB International, UK, pp 165–183
Chung J, Lee J-H, Arumuganathan K, Graef GL, Specht JE (1998) Relationships between nuclear DNA content and seed and leaf size in soybean. Theor Appl Genet 96:1064–1068
D’Onofrio C, Morini C, Bellocchi G (1998) Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tissue Organ Cult 53:91–98
De Schepper S, Leus L, Mertens M, Van Bockstaele M, De Loose M (2001) Flow cytometric analysis of ploidy in Rhododendron (subgenus Tsutsusi). Hortscience 36:125–127
De Veylder L, Beeckman T, Beemster GTS, De Almeida Engler J, Ormenese S, Maes S, Naudts M, Van Der Schueren E, Jacqmard A, Engler G, Inzé D (2002) Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa–DPa transcription factor. EMBO J 21:1360–1368
Gendreau E, Orbovic V, Hofte H, Traas J (1998) Phytohormone controls the number of endoreduplication cycles in the Arabidopsis thaliana hypocotyls. Plant J 13:221–230
Gendreau E, Orbovic V, Hofte H, Traas J (1999) Gibberellin and ethylene control endoreduplication levels in the Aradopsis thaliana hypocotyls. Planta 209:513–516
Heo JW, Lee CW, Paek KY (2006) Influence of mixed LED radiation on the growth of annual plants. J Plant Biol 49:286–290
Jones WE, Kuehnle AR (1998) Ploidy identification using flow cytometry in tissues of Dendrobium species and cultivars. Lindleyana 13:11–18
Jones WE, Kuehnle AR, Arumuganathan K (1998) Nuclear DNA content of 26 orchid (Orchidaceae) genera with emphasis on Dendrobium. Ann Bot 82:189–194
Joubes J, Chevalier L (2000) Endoduplication in higher plants. Plant Mol Biol 43:735–745
Kinoshita I, Sanbe A, Yokomuraz E-L (1991) Increases in nuclear DNA content without mitosis in benzyladenine-treated primary leaves of intact and decapitated bean plants. J Exp Bot 42:667–672
Kladnik A, hourey PS, Pring DR, Dermastia M (2006) Development of the endosperm of Sorghum bicolor during the endoreduplication-associated growth phase. J Cereal Sci 43:209–215
Kudo N, Kimura Y (2001) Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species. Theor Appl Genet 102:104–110
Larkins BA, Dilkes BP, Dante RA, Coelho CM (2001) Investigating the hows and whys of DNA endoreduplication. J Exp Bot 52: 183–192
Lee HC, Chiou DW, Chen WH, Markhart AH, Chen YH, Lin TY (2004) Dynamics of cell growth and endoreduplication during orchid flower development. Plant Sci 166:659–667
Lee HC, Chen YJ, Markhart AH, Lin TY (2007) Temperature effects on systemic endoreduplication in orchid during floral development. Plant Sci 172:588–595
Levan A (1939) Cytological phenomena connected with the root swelling caused by growth substances. Hereditas 25:87–96
Lian ML, Murthy HN, Paek KY (2006) Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Process Biochem 41:1179–1185
Melaragno JE, Mehrotra B, Coleman AW (1993) Relationship between endopolyploidy and cell size in epidermal tissues of Arabidopsis. Plant Cell 5:1661–1668
Muleo R, Morini S (1990) Effect of light quality on regeneration from callus of Actinidia deliciosa. Acta Hortic 280:155–158
Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tissue Organ Cult 73:43–52
Park SY, Paek KY (1999) Occurrence of abnormal plants and their morphological characteristics as affected by clones and culture periods in clonally propagated Phalaenopsis by leaf culture. J Korean Soc Hortic Sci 40:731–734
Park SY, Paek KY (2006) Endoreduplication pattern of somatic embryos and variants occurrence affected by pre-existed endoreduplicated cells in Doritaenopsis. Korean J Plant Biotechnol 33:297–302
Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm-like bodies (PLBs) using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tissue Organ Cult 63:67–72
Park SY, Murthy HN, Paek KY (2002) Rapid propagation of Phalaenopsis from floral stalk derived leaves. In Vitro Cell Dev Biol Plant 38:168–172
Pierik RLM (1987) Vegetative propagation of orchids. In: Pierik RLM (ed) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, pp 159–169
Rival A, Beule T, Barre P, Hamon S, Ynoirot YD (1997) Comparative flow cytometric estimation of nuclear DNA content in oil palm (Elaeis guineensis Jacq.) tissue cultures and seed-derived plants. Plant Cell Rep 16:884–887
Shohael SM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41:1179–1185
Sugimoto-Shiraru K, Roberts K (2003) “Big it up”: endoreduplication and cell-size control in plants. Curr Opin Plant Biol 6:544–553
Sun Y, Flannigan BA, Setter TL (1999) Regulation of endoduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type cyclin and its quantitative analysis. Plant Mol Biol 41: 245–258
Tokuhara K, Mii M (2001) Induction of embryogenic callus and cell suspension culture from shot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cell Dev Biol Plant 37:457–461
Torne JM, Moysset L, Santos M, Simón E (2001) Effects of light quality on somatic embryogenesis in Araujia sericifera. Physiol Plant 111:405–411
Yeung EC (1999) The use of histology in the study of plant tissue culture systems—some practical comments. In Vitro Cell Dev Biol Plant 35:137–143
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The research was supported by Basic Science Research Program through the National Research Foundation (NRF) by the Ministry of Education, Science and Technology.
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Park, SY., Yeung, E.C. & Paek, KY. Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis. Plant Biotechnol Rep 4, 303–309 (2010). https://doi.org/10.1007/s11816-010-0148-x
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DOI: https://doi.org/10.1007/s11816-010-0148-x