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Production and characterization of biosurfactant from marine bacterium Inquilinus limosus KB3 grown on low-cost raw materials

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Abstract

Inquilinus limosus strain KB3, isolated from marine sediment in the south of Thailand, was used to produce a biosurfactant from a mineral salts medium (MSM) with palm oil decanter cake (PODC) as a carbon source. It was found that cellular growth and biosurfactant production in MSM were greatly affected by the medium components. I. limosus KB3 was able to grow and to produce surfactant reducing the surface tension of medium to 28.2 mN/m and giving a crude surfactant concentration of 5.13 g/l after 54 h. The biosurfactant obtained was found to reduce the surface tension of pure water to 25.5 mN/m with the critical micelle concentration of 9 mg/l, and retained its properties during exposure to elevated temperatures (121 °C), high salinity (12 % NaCl), and a wide range of pH values. Chemical characterization by FT-IR, NMR, and ESI-MS revealed that the biosurfactant has a lipopeptide composition with molecular mass (m/z) of 1,032. The biosurfactant was capable of forming stable emulsions with various hydrocarbons and had the ability to enhance oil recovery, PAHs solubility, and antimicrobial activity.

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Acknowledgments

We are grateful to Phuket Rajabhat University for providing a scholarship to A.S. This work was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission and the Postdoctoral Fellowship from Prince of Songkla University. The work was also funded by the Graduate School, Prince of Songkla University.

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Authors and Affiliations

  1. Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai & Satianpong Udomsilp

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Suppasil Maneerat

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  1. Atipan Saimmai
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  2. Satianpong Udomsilp
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  3. Suppasil Maneerat
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Correspondence to Atipan Saimmai.

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Saimmai, A., Udomsilp, S. & Maneerat, S. Production and characterization of biosurfactant from marine bacterium Inquilinus limosus KB3 grown on low-cost raw materials. Ann Microbiol 63, 1327–1339 (2013). https://doi.org/10.1007/s13213-012-0592-7

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  • DOI: https://doi.org/10.1007/s13213-012-0592-7

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