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Characterization of Rhamnolipid Produced by Pseudomonas aeruginosa Isolate Bs20

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Abstract

Rhamnolipid produced by Pseudomonas aeruginosa isolate Bs20 is viscous sticky oily yellowish brown liquid with a fruity odor. It showed solubility at aqueous pH > 4 with optimum solubility at pH 7–7.5 and freely soluble in ethyl acetate. This biosurfactant has a very high surface activity as it could lower the surface tension of water to 30 mN/m at about 13.4 mg/L, and it exhibited excellent stabilities at high temperatures (heating at 100°C for 1 h and autoclaving at 121°C for 10 min), salinities (up to 6% NaCl), and pH values (up to pH 13). The produced biosurfactant can be used in the crude form either as cell-free or cell-containing culture broth of the grown bacteria, since both preparations showed high emulsification indices ranged between 59% and 66% against kerosene, diesel, and motor oil. These characters make the test rhamnolipid a potential candidate for use in bioremediation of hydrocarbon-contaminated sites or in the petroleum industry. High-performance thin-layer chromatography densitometry revealed that the extracted rhamnolipid contained the two most active rhamnolipid homologues dirhamno dilipidic rhamnolipid and monorhamno dilipidic rhamnolipid at 44% and 56%, respectively, as compared to 51% and 29.5%, respectively, in a standard rhamnolipid preparation. The nature and ratio of these two rhamnolipid homologues showed to be strain dependent rather than medium-component dependent.

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Acknowledgments

The authors would like to thank Dr. Salam M. Awada (AgSciTech, Logan, UT, USA) for kindly supplying the standard rhamnolipid and Dr. Anas M. Abdel-Mawgoud (ARCMP, NODCAR, Ministry of Health, Cairo, Egypt) for technical support and help in HPTLC experiments.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Al Khalifa Al Maamoun St., Abbassia, Cairo, Egypt

    Ahmad Mohammad Abdel-Mawgoud, Mohammad Mabrouk Aboulwafa & Nadia Abdel-Haleem Hassouna

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  1. Ahmad Mohammad Abdel-Mawgoud
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  2. Mohammad Mabrouk Aboulwafa
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  3. Nadia Abdel-Haleem Hassouna
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Correspondence to Mohammad Mabrouk Aboulwafa.

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Abdel-Mawgoud, A.M., Aboulwafa, M.M. & Hassouna, N.AH. Characterization of Rhamnolipid Produced by Pseudomonas aeruginosa Isolate Bs20. Appl Biochem Biotechnol 157, 329–345 (2009). https://doi.org/10.1007/s12010-008-8285-1

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  • DOI: https://doi.org/10.1007/s12010-008-8285-1

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