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Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7T and R19T, were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10–37°C and pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Strain R7T contained iso-C14:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1ω9c), whereas strain R19T contained iso-C11:0 3-OH, C16:1ω7c alcohol, iso-C11:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1ω9c) as major cellular fatty acids (> 5%). Both strains contained ubiquin-one-8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7T and R19T calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7T and R19T were most closely related to Lysobacter panacisoli C8-1T and Lysobacter niabensis GH34-4T with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7T and R19T formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7T and R19T represent novel species of the genus Lysobacter, for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7T (= KACC 21663T = JCM 34257T) and R19T (= KACC 21767T = JCM 34258T), respectively.

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Acknowledgements

This work was supported by the Chung-Ang University Research Scholarship Grants in 2020 and the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR No. 2020-02-001) of the Ministry of Environment (MOE), Republic of Korea. We thank Prof. Aharon Oren for the valuable support on the nomenclature and etymology of strains R7T and R19T.

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  1. Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea

    Kyeong Ryeol Kim, Kyung Hyun Kim, Shehzad Abid Khan, Hyung Min Kim, Dong Min Han & Che Ok Jeon

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of strains R7T and R19T are MN197915 and CP071517 and MW173243 and CP071518, respectively.

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Kim, K.R., Kim, K.H., Khan, S.A. et al. Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil. J Microbiol. 59, 709–717 (2021). https://doi.org/10.1007/s12275-021-1156-y

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