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Lysobacter caseinilyticus, sp. nov., a casein hydrolyzing bacterium isolated from sea water

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Abstract

A novel bacterial strain, designated KVB24T, was isolated from sea-water of Busan Harbour in South Korea. Cells of strain KVB24T were Gram-stain negative, aerobic, rod shaped and non-motile. Strain KVB24T grew optimally at 25–28 °C and pH 6.5–7.0. Based on 16S rRNA gene sequence analysis, strain KVB24T was shown to belong to the genus Lysobacter within the class Gammaproteobacteria and to be closely related to Lysobacter dokdonensis DS-58T, Lysobacter hankyongensis KTce-2T and Lysobacter niastensis GH41-7T. DNA–DNA relatedness between strain KVB24T and its current closest relative was below 70%. The predominant fatty acids of strain KVB24T were iso-C11:0, iso-C11:0 3-OH, iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0 and summed feature 9 comprising (iso-C17:1 ω9c and/or 10 methyl C16:0); the prominent isoprenoid was Q-8 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G + C content of genomic DNA from strain KVB24T was determined to be 67.5 mol%. Based on the phenotypic, genotypic and chemotaxonomic analyses, strain KVB24T represents a novel species of the genus Lysobacter, for which the name Lysobacter caseinilyticus sp. nov. is proposed. The type strain is KVB24T (= KACC19816T = JCM32879T).

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Acknowledgements

This work was supported by the National Institute of Environmental Research, Republic of Korea. We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for the suggested genus and species names.

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  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea

    Geeta Chhetri, Jiyoun Kim, Inhyup Kim & Taegun Seo

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  1. Geeta Chhetri
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Correspondence to Taegun Seo.

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The GenBank accession number for the 16S rRNA gene sequence of strain KVB24T is MK177529.

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Chhetri, G., Kim, J., Kim, I. et al. Lysobacter caseinilyticus, sp. nov., a casein hydrolyzing bacterium isolated from sea water. Antonie van Leeuwenhoek 112, 1349–1356 (2019). https://doi.org/10.1007/s10482-019-01267-7

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