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The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Peanut (Arachis hypogaea. L) is an important oil seed crop. Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium (Ca2+) application can ameliorate the impact of saline soil on peanut production, and the rhizosphere bacterial communities are also closely correlated with peanut salt tolerance; however, whether AMF and Ca2+ can withstand high-salinity through or partially through modulating rhizosphere bacterial communities is unclear. Here, we used the rhizosphere bacterial DNA from saline alkali soil treated with AMF and Ca2+ alone or together to perform high-throughput sequencing of 16S rRNA genes. Taxonomic analysis revealed that AMF and Ca2+ treatment increased the abundance of Proteobacteria and Firmicutes at the phylum level. The nitrogen-fixing bacterium Sphingomonas was the dominant genus in these soils at the genus level, and the soil invertase and urease activities were also increased after AMF and Ca2+ treatment, implying that AMF and Ca2+ effectively improved the living environment of plants under salt stress. Moreover, AMF combined with Ca2+ was better than AMF or Ca2+ alone at altering the bacterial structure and improving peanut growth in saline alkali soil. Together, AMF and Ca2+ applications are conducive to peanut salt adaption by regulating the bacterial community in saline alkali soil.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31771732, 31901574, and 31971856), National Key R&D program of China (Grant No. 2018YFE0108600), and Major Scientific and Technological Innovation Projects in Shandong Province (Grant No. 2019JZZY010702).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, Shandong, 266100, P. R. China

    Dunwei Ci, Hong Ding, Guanchu Zhang, Shangxia Li, Liangxiang Dai, Feifei Qin, Zhimeng Zhang, Jishun Yang & Yang Xu

  2. Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Shandong Key Lab. of Genetic Improvement, Ecology and Physiology of Crops, Jinan, 250100, P. R. China

    Zhaohui Tang

  3. Shandong Provincial Crop Germplasm Resource Centre, Shandong Academy of Agricultural Sciences, Jinan, 250100, P. R. China

    Li Cui

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  1. Dunwei Ci
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  2. Zhaohui Tang
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  3. Hong Ding
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  4. Li Cui
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  5. Guanchu Zhang
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  9. Zhimeng Zhang
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  11. Yang Xu
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Correspondence to Jishun Yang or Yang Xu.

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Ci, D., Tang, Z., Ding, H. et al. The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil. J Microbiol. 59, 51–63 (2021). https://doi.org/10.1007/s12275-021-0317-3

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