Abstract
Phosphate solubilizing microorganisms are ubiquitous in soils and could play an important role in supplying P to plants where plant unavailable P content in soil was more. A phosphatase and phytase producing fungus Emericella rugulosa was isolated and tested under field condition (Pearl millet as a test crop) in a loamy sand soil. In the experimental soil 68% organic phosphorous was present as phytin; less than 1% of phosphorous was present in a plant available form. The maximum effect of inoculation on different enzyme activities (acid phosphatase, alkaline phosphatase, phytase, and dehydrogenase) was observed between 5 and 8 weeks of plant age. The depletion of organic P was much higher than mineral and phytin P. The microbial contribution was significantly higher than the plant contribution to the hydrolysis of the different P fractions. A significant improvement in plant biomass, root length, seed and straw yield and P concentration of root and shoot resulted from inoculation. The results suggest that Emericella rugulosa produces phosphatases and phytase, which mobilize P and enhance the production of pearl millet.
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References
Rodriguez H & Fraga R (1990) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339
Sanyal S K & De Datta S K (1991) Chemistry of phosphorus transformation in soil. Adv Soil Sci 16: 1–120
Tarafdar JC & Claassen N (1988) Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms. Biol Fert Soils 5:308–312
Tarafdar JC, Yadav RS & Niwas R (2002) Relative efficiency of fungal intra-and extracellular phosphatases and phytase. J Plant Nutr Soil Sci 165:7–20
Mcgill WB & Cole CV (1981) Comparative aspects of C, N, S and P cycling through soil organic matter during pedogenesis. Geoderma 26:267–286
Richardson AE (1994) Soil microorganisms and phosphorus availability. In: Soil Biota Management in Sustainable Farming Systems, (Pankhurst CE, Doulse BM, Gupta VVSR & Grace PR eds). CSIRO, Australia, pp 50–62
Whitelaw MA, Harden TJ & Helyar K (1999) Phosphate solubilisation in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31: 655–665
Tarafdar JC & Marschner H (1995) Dual inoculation with Aspergillus fumigatus and Glomus mossae enhances biomass production and nutrient uptake in wheat (Triticum aestivum L.) supplied with organic phosphorus as Na-phytate. Plant Soil 173:97–102
Wyss M, Brugger R, Kronenberger A, Remy R, Fimbel R, Oesterhelt G, Lehmann M & Loon AP van (1999) Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microbiol 65:367–373
Yadav RS & Tarafdar JC (2003) Phytase and phosphatase producing fungi in arid and semi-arid soils and their efficiency in hydrolyzing different organic P compounds. Soil Biol Biochem 35:745–751
Tarafdar JC & Chhonkar PK (1979) Phosphatase production by microorganisms isolated from diverse types of soils. Zbl Bakt 134:119–122
Allen ON (1959) Experiments in soil bacteriology. Burgess Publishing Co, 117 p.
Tennant D (1975) A test of a modified line intersects method of estimating root length. J Ecol 63: 995–1001
Kitson RE & Mellon MG (1944) Colorimetric determination of phosphorus molybdovanadophosphoric acid. Ind Eng Chem Anal Ed 16:379–383
Tabatabai MA & Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307
Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. Method Enzymol 8:115–118
Tabatabai MA (1982) Soil enzymes. In: Methods of Soil Analysis, Part 2 (Page AL, Miller RH & Kenney DR eds). Am Soc Agron, Madison, Wisconsin, USA, pp 903–947
Jackson ML (1967) Soil Chemical Analysis. Prentice-Hall of India, Delhi, 498 p.
Sokal RR & Roholf FJ (1981) Biometry-The Principles and Practice of Statistics in Biological Research. W.H. Freeman and Co, New York
Herrera MA, Salamanka CP & Barea JM (1993) Inoculation of woody legumes with selected arbuscular mycorrhizal fungi and rhizobia to recover desertified Mediterranean ecosystems. Appl Environ Microbiol 59:129–133
Glick BR (1995) The enhancement of plant growth by free living bacteria. Can J Microbiol 41:109–117
Requena BN, Jimenez I, Toro M & Barea JM (1997) Interactions between plant growth promoting rhizobacteria (PGPR), arbuscular mycorrhizal fungi and Rhizobium spp. in the rhizosphere of Anthyllis cytiisoides, a model legume for revegetation in Mediterranean semi-arid ecosystem. New Phytologist 136:667–677
Hinsinger P (1998) How do plant roots acquire mineral nutrients Chemical processes involved in the rhizosphere. Adv. Agron. 64:225–265
Lajtha K & Harrison AF (1995) Strategies of phosphorus acquisition and conservation by plant species and communities. In: Phosphorus in the Global Environment-Transfers, Cycles and Management (Tiessen H ed), John Wiley and Sons, Chichester, pp 139–147
Hübel F & Beck E (1993) In-situ determination of the P-relations around the primary root of maize with respect to inorganic and phytase-P. Plant Soil 157:1–9
Hinsinger P & Gilkes RJ (1997) Dissolution of phosphate rock in the rhizosphere of fine plant species grown in an acid P-fixing mineral substrate. Geoderma 75:231–249
Barber SA (1984) Soil Nutrient Bioavailability. A Mechanistic Approach. John Wiley and Sons, New York
Dalal RC (1978) Soil organic phosphorus. Adv Agron 29:83–117
Yadav RS & Tarafdar JC (2001) Influence of organic and inorganic phosphorus supply on the maximum secretion of acid phosphatase by plants. Biol Fertil Soils 34:140–143
Tarafdar JC (1989) Use of electrofocussing technique for characterizing the phosphatases in the soil and root exudates. J Ind Soc Soil Sci 37:393–395
Li M, Osaki M, Honma M & Tadano T (1997) Purification and characterization of phytase induced in tomato roots under phosphorus deficient conditions. Soil Sci Plant Nutr 43:179–190
Tarafdar JC & Marschner H (1994) Phosphatase activity in the rhizosphere and hyphosphere of a VA mycorrhizal wheat supplied with inorganic and organic phosphorus. Soil Biol Biochem 26:387–395
Skujins J (1973) Dehydrogenase activity: an indicator of biological activity in arid soils. Bull Ecol Res Comm 17:235–241
Greaves MP & Webley DM (1969) The hydrolysis of myoinositol hexaphosphate by soil microorganisms. Soil Biol Biochem 1:37–43
Jones D L (1998) Organic acids in the rhizosphere — a critical review. Plant Soil 205:25–44
Yadav B K & Tarafdar J C (2004) Phytase activity in the rhizosphere of crops, trees and grasses under arid environment. J Arid Environ 56:285–293
Richardson A E, Hadobas P A, Hayes J E, O’ Hara C P & Simpson R J (2001) Utilization of phosphorus and pasture plants supplied with myo-inositol hexaphosphate is enhanced by the presence of soil micro-organisms. Plant Soil 229:47–56
Neumann G & Römheld V (2000) The release of root exudates as affected by the plant’s physiological status. In: The rhizosphere, biochemistry and organic substances at the soil-plant interface (Pinton R, Varanini Z & Nannipieri P eds). Marcel Dekker Inc, pp 41–93
Kucey R M N, Janzen H H & Leggett M E (1989) Microbially mediated increases in plant-available phosphorus. Adv Agron 42:199–228
Rao A V, Bala K & Tarafdar JC (1990) Dehydrogenase and phosphatase activities in soil as influenced by the growth of arid-land crops. J Agric Sci 115:221–225
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Yadav, B.K., Tarafdar, J.C. Ability of Emericella rugulosa to mobilize unavailable P compounds during Pearl millet [Pennisetum glaucum (L.) R. Br.] crop under arid condition. Indian J Microbiol 47, 57–63 (2007). https://doi.org/10.1007/s12088-007-0011-0
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DOI: https://doi.org/10.1007/s12088-007-0011-0