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An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

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Abstract

Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.

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Abbreviations

FAM:

6-Carboxyfluorescein

ATCC:

American Type Culture Collection

FluMag-SELEX:

Aptamer fluorescent labeling and magnetic beads immobilization

AM-ECL:

Aptamer-magnetic bead-electrochemiluminescence

CE:

Capillary electrophoresis–SELEX

CDC:

Centers for Disease Control and Prevention

dPa:

Deoxyribonucleoside triphosphate 4-propynylpyrrole-2-carbaldehyde

Px:

Diol-modified 2-nitro-4-propynylpyrrole

DCE:

DNA capture element

ELASA:

Enzyme-linked aptamer sedimentation assay

ECDC:

European Centre for Disease Prevention and Control

FITC:

Fluorescein isothiocyannate

FRET:

Fluorescence resonance energy transfer

FACS:

Fluorescence-activated cell sorting

HTS:

High-throughpuT-SELEX

Ds:

Hydrophobic base 7-(2-thienyl) imidazo[4,5-b]pyridine

MREs:

Molecular recognition elements

NECEEM:

Non-equilibrium capillary electrophoresis of equilibrium mixtures

OMPs:

Outer membrane proteins

QD:

Quantum dot

SELEXp:

Systematic evolution of ligands by exponential enrichment

SEA:

Staphylococcus aureus enterotoxin A

SEB:

Staphylococcus aureus enterotoxin B

SERS:

Surface-enhanced Raman spectroscopy

TECS-SELEX:

Target expressed on cell surface-SELEX

UCNPs:

Up conversion nanoparticles

ROX:

X-rhodamine

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Omar Mukama, Jean Paul Sinumvayo, Henriette Mushimiyimana, Leo Bemena & Samson Mugisha

  2. Department of Applied Biology, College of Science and Technology University of Rwanda, Avenue de l’armée, P.O. Box: 3900, Kigali, Rwanda

    Omar Mukama & Peter Rwibasira

  3. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Muhammad Shamoon, Muhammad Shoaib, Waseem Safdar & Zhouping Wang

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  1. Omar Mukama
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Omar Mukama declares that he has no conflict of interest. Jean Paul Sinumvayo declares that he has no conflict of interest. Muhammad Shamoon declares that he has no conflict of interest. Muhammad Shoaib declares that he has no conflict of interest. Henriette Mushimiyimana declares that he has no conflict of interest. Waseem Safdar declares that he has no conflict of interest. Leo Bemena declares that he has no conflict of interest. Peter Rwibasira declares that he has no conflict of interest. Samson Mugisha declares that he has no conflict of interest. Zhouping Wang declares that he has no conflict of interest.

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Mukama, O., Sinumvayo, J.P., Shamoon, M. et al. An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens. Food Anal. Methods 10, 2549–2565 (2017). https://doi.org/10.1007/s12161-017-0814-5

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