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Sugarcane Bagasse Hydrothermal Pretreatment Liquors as Suitable Carbon Sources for Hemicellulase Production by Aspergillus niger

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Abstract

The aim of this study was to valorize the hemicellulose-rich liquid fraction (liquor) arising from hydrothermal pretreatment of sugarcane bagasse (SCB) through its utilization as an unconventional, soluble carbon source for the production of hemicellulases, namely xylanases and α-L-arabinofuranosidases (ABFases), by Aspergillus niger DCFS11. Through the use of factorial design, pretreatment conditions producing liquors optimized for either early- or late-phase enzyme production were identified. Subsequent deep characterization of liquor components using liquid chromatography and mass spectrometry was performed to identify compounds likely responsible for hemicellulase induction. SCB liquors arising from various pretreatment configurations induced up to 2- and 8.6-fold higher xylanase and ABFase production, respectively, by A. niger DCFS11 than raw SCB substrate owing to the strong inducing potential of arabinosylated xylooligosaccharides and free arabinose solubilized during pretreatment. Notably, unlike the severe pretreatment conditions required for maximum cellulose saccharification and ethanol yields during biomass conversion, low severity and low biomass loading are required if enzyme production from liquor is desired at early-phase growth with no additional detoxification steps. This suggests that for effective application in biorefineries, separate or multi-step processes would be required to optimize both hemicellulase production by A. niger DCFS11 and cellulose digestion. This work demonstrates the potential of hydrothermal pretreatment of lignocellulosic substrates as a tool to increase the production of enzymes by filamentous fungi.

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Funding

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Foundation for Research Support of the Federal District (FAPDF), and the National Center for Agroenergy Research – Brazilian Enterprise for Agricultural Research (EMBRAPA).

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Authors and Affiliations

  1. Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil

    Caio de Oliveira Gorgulho Silva & Edivaldo Ximenes Ferreira Filho

  2. National Center for Agroenergy Research, Brazilian Enterprise for Agricultural Research (EMBRAPA), Parque Estação Biológica, PqEB s/n°, Avenida W3 Norte, Brasília, DF, 70770-901, Brazil

    José Antonio de Aquino Ribeiro, Augusto Lopes Souto & Patrícia Verardi Abdelnur

  3. Institute of Chemistry, Federal University of Goiás, Campus Samambaia, Goiânia, GO, 74690-900, Brazil

    Patrícia Verardi Abdelnur

  4. Department of Food Sciences, Federal University of Lavras, Lavras, MG, 37200-00, Brazil

    Luís Roberto Batista

  5. Grupo de Engenharia Metabólica Aplicada a Bioprocessos, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil

    Kelly Assis Rodrigues & Nádia Skorupa Parachin

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  1. Caio de Oliveira Gorgulho Silva
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  8. Edivaldo Ximenes Ferreira Filho
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Correspondence to Caio de Oliveira Gorgulho Silva.

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de Oliveira Gorgulho Silva, C., de Aquino Ribeiro, J.A., Souto, A.L. et al. Sugarcane Bagasse Hydrothermal Pretreatment Liquors as Suitable Carbon Sources for Hemicellulase Production by Aspergillus niger. Bioenerg. Res. 11, 316–329 (2018). https://doi.org/10.1007/s12155-018-9898-z

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  • DOI: https://doi.org/10.1007/s12155-018-9898-z

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