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Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation

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Abstract

In Mexico, the generation of agave bagasse (AB) has increased considerably in recent years, given the growing global demand for tequila and other products from agave plants; around 45 thousand tons of AB are produced monthly only from tequila production. Despite the potential of AB to produce high-value metabolites, its use is limited. Several efforts have been made to mitigate the environmental problem caused by the final disposition of AB; however, the use of AB to produce enzymes has not been widely explored. This work presents a comparative analysis of the bioconversion of AB by Aspergillus fumigatus and Penicillium citrinum to produce cellulases and xylanases by solid-state fermentation, using wheat straw (WS) and sugarcane bagasse (SCB) as control feedstocks. The highest cellulase productions were obtained on AB with 10,112 and 7,347 U/kg of AB by P. citrinum and A. fumigatus, respectively. Regarding the xylanase production, the best producer was A. fumigatus (125,250 U/kg of WS), while the maximum xylanase production on AB was 28,974 U/kg of AB by P. citrinum. These results show the promising potential of AB to produce lignocellulase enzymes and open the vision towards implementing a circular economy strategy around the agave plant transformation.

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Acknowledgments

The authors would like to thank the National Council of Science and Technology and Secretariat of Energy of Mexico for supporting this study through the Energy Sustainability Fund (FSE CONACYT-SENER). We thank our colleague Dr. Ángeles Calixto Romo from El Colegio de la Frontera Sur for the kindly donation of the fungus Penicillum citrinum CGETCR. We would also like to thank Montserrat del Socorro Valle Pérez for digital illustration of Fig. 6 and graphical abstract.

Funding

This study was funded by the Energy Sustainability Fund CONACYT-SENER [Project number 245750]. A.U. Valle-Pérez received a grant from the project 245750.

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  1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco AC, Unidad Zapopan, Camino El Arenero 1227, El Bajío del Arenal, C.P. 45019, Zapopan, Jalisco, México

    A. U. Valle-Pérez, G. Flores-Cosío & L. Amaya-Delgado

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Conceptualization, methodology, formal analysis and investigation, writing-original draft preparation, supervision: Lorena Amaya-Delgado and Alexander Uriel Valle-Pérez. Writing-review and editing: Lorena Amaya-Delgado, Guillermo Flores-Cosío and Alexander Uriel Valle-Pérez. Resources: Lorena Amaya-Delgado.

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Correspondence to L. Amaya-Delgado.

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Valle-Pérez, A.U., Flores-Cosío, G. & Amaya-Delgado, L. Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation. Waste Biomass Valor 12, 5885–5897 (2021). https://doi.org/10.1007/s12649-021-01397-y

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