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Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties

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Abstract

Economic interest in sugarcane bagasse has significantly increased in recent years due to the worldwide demand for sustainable energy production. The use of sugarcane bagasse for holocellulase production has been a strategy for bioconversion of lignocellulosic residues into second-generation ethanol. The fungi secrete to the culture medium a cocktail of enzymes necessary to convert biomass into nutrients. Thus, this study aimed to analyze the production profile of holocellulases from Aspergillus and Humicola species, Trichoderma reesei RP698, and Mycothermus thermophilus grown in sugarcane bagasse, culm of energy cane, and culm of sugarcane SP80-3280. The capacity of the enzymatic pools in the hydrolysis of cell walls of these sugarcane varieties was also verified. M. thermophilus was the best producer of endoglucanase, cellobiohydrolase, β-glucosidase, xylanase, β-xylosidase, xyloglucanase, arabinanase, arabinofuranosidase, mannanase, and acetyl xylan esterase. T. reesei RP698 also produced and secreted a wide range of holocellulases to the medium. The saccharification of sugarcane bagasse, energy cane, and sugarcane SP80-3280 by the enzymatic cocktails obtained from M. thermophilus released 0.87 ± 0.05 mg.mL−1, 0.88 ± 0.07 mg.mL−1, and 1.10 ± 0.08 mg.mL−1 of reducing sugars, respectively. However, the application of T. reesei RP698 extracts showed a release of 0.85 ± 0.03 mg.mL−1, 0.40 ± 0.03 mg.mL−1, and 0.83 ± 0.03 mg.mL−1 of reducing sugars. Therefore, T. reesei RP698 and M. thermophilus showed to be good holocellulase producers, and their crude extracts presented a great capacity for the hydrolysis of the different kinds of sugarcane residues.

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Acknowledgements

This study was financed by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, processes 2014/50884-5, 10/52322-3, 2018/07522-6), and Conselho de Desenvolvimento Científico e Tecnológico (CNPq, process 46.5319/2014-9). This work was partially supported by the National Institute of Science and Technology of Bioethanol – INCT do Bioetanol (FAPESP/CNPq) and AG (FAPESP 2019/13936-0). MLTMP is a research productivity fellow of CNPq (process 301963/2017-7). We thank Mauro Xavier (Centro de Cana – IAC, Ribeirão Preto – SP, Brazil) and Monalisa Sampaio Carneiro (CCA – UFSCar, Araras – SP, Brazil) for providing the biomass for this research. We thank Mariana Cereia and Mauricio de Oliveira for their technical assistance.

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Authors and Affiliations

  1. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo, Ribeirão Preto, SP, Brazil

    Ana Sílvia de Almeida Scarcella, Thiago Machado Pasin, Rosymar Coutinho de Lucas, Alex Graça Contato & Maria de Lourdes Teixeira de Moraes Polizeli

  2. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo, Ribeirão Preto, SP, Brazil

    Rosymar Coutinho de Lucas, Monica Stropa Ferreira-Nozawa, Tássio Brito de Oliveira & Maria de Lourdes Teixeira de Moraes Polizeli

  3. Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil

    Adriana Grandis & Marcos Silveira Buckeridge

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  1. Ana Sílvia de Almeida Scarcella
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  2. Thiago Machado Pasin
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  9. Maria de Lourdes Teixeira de Moraes Polizeli
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Highlights

• Mesophilic and thermophilic fungi have high potential for holocellulase production.

• Sugarcane bagasse and energy cane are good inductors for enzyme production.

• Mycothermus thermophilus and Trichoderma reesei showed high holocellulase levels.

• M. thermophilus and T. reesei improved the hydrolysis efficiency on biomass.

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Scarcella, A.S.d., Pasin, T.M., de Lucas, R.C. et al. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties. Biomass Conv. Bioref. 13, 1163–1174 (2023). https://doi.org/10.1007/s13399-021-01304-4

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