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Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger

  • Bioenergy/Biofuels/Biochemicals - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger’s secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.

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Acknowledgements

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Foundation for Research Support of the Federal District (FAPDF, Grant PRONEX 0193.001195/2016). Funding was also provided by the Funding Authority for Studies and Projects (FINEP/CT-INFRA, Grants 0439/2011 and 0694/2013) to MVS.

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Authors and Affiliations

  1. Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil

    Caio de Oliveira Gorgulho Silva, Ricardo Henrique Krüger & Edivaldo Ximenes Ferreira Filho

  2. Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil

    Agenor de Castro Moreira dos Santos Júnior, Wagner Fontes, Marcelo Valle de Sousa & Carlos André Ornelas Ricart

  3. Instituto Federal de Brasília, Brasília, DF, 73380-900, Brazil

    Renata Henrique Santana

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  1. Caio de Oliveira Gorgulho Silva
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  2. Agenor de Castro Moreira dos Santos Júnior
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  3. Renata Henrique Santana
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Correspondence to Caio de Oliveira Gorgulho Silva.

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de Oliveira Gorgulho Silva, C., de Castro Moreira dos Santos Júnior, A., Santana, R.H. et al. Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger. J Ind Microbiol Biotechnol 46, 1517–1529 (2019). https://doi.org/10.1007/s10295-019-02207-0

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