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Yeast Expression Systems: Overview and Recent Advances

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Abstract

Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Currently, several new systems are commercially available; some of them are private and need licensing. The potential for secretory expression of heterologous proteins in yeast proposed this system as a candidate for the production of complex eukaryotic proteins. The common yeast expression hosts used for recombinant proteins’ expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. This review is dedicated to discuss on significant characteristics of the most common methylotrophic and non-methylotrophic yeast expression systems with an emphasis on their advantages and new developments.

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Acknowledgements

This study was supported by a Grant from the Biotechnology Research Center Tabriz University of Medical Science.

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Authors and Affiliations

  1. Medical Biotechnology Department, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Roghayyeh Baghban

  2. Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Roghayyeh Baghban

  3. Biotechnology Research Center, Tabriz University of Medical Sciences, Daneshgah Ave, Tabriz, Iran

    Roghayyeh Baghban, Safar Farajnia & Maryam Aria

  4. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Safar Farajnia

  5. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Velenjak, Arabi Ave, Tehran, Iran

    Masoumeh Rajabibazl

  6. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Masoumeh Rajabibazl & Reyhaneh Hoseinpoor

  7. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Science, Shiraz, Iran

    Younes Ghasemi

  8. Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    AmirAli Mafi

  9. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Leila Rahbarnia

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  1. Roghayyeh Baghban
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Baghban, R., Farajnia, S., Rajabibazl, M. et al. Yeast Expression Systems: Overview and Recent Advances. Mol Biotechnol 61, 365–384 (2019). https://doi.org/10.1007/s12033-019-00164-8

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