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Expression of Recombinant Proteins in Pichia Pastoris

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Abstract

Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.

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Acknowledgments

We thank Dr. Geoff Lin-Cereghino (Department of Biology, University of the Pacific) for helpful comments on the manuscript, and Mr. Neal Johnson (School of Dentistry, University of the Pacific) for preparing the figures. The research described here was supported by US AFOSR, NSF, the Wallace Coulter Foundation, and Harvard Medical School.

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Authors and Affiliations

  1. Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, 200233, People’s Republic of China

    Pingzuo Li

  2. Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

    Pingzuo Li & V. Renugopalakrishnan

  3. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115, USA

    Anukanth Anumanthan

  4. Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, 37 Convent Drive, 37/1B23, Bethesda, MD, 20892, USA

    Xiu-Gong Gao

  5. Centro de Investigacion en Calidad Ambiental, Tecnologico de Monterrey, Campus Estado de México, Monterrey, Mexico

    Kuppusamy Ilangovan

  6. Department of Microbiology, Arthur A. Dugoni School of Dentistry, University of the Pacific, 2155 Webster Street, San Francisco, CA, 94115, USA

    Vincent V. Suzara & Nejat Düzgüneş

  7. Bionanotechnology Group, Florida International University, Miami, FL, 33174, USA

    V. Renugopalakrishnan

  8. Biophotovoltaic Group, Division of Bioengineering and Department of Mechanical Engineering, NUS Nanotechnology Initiative (NUSNNI), National University of Singapore, Singapore, -117576, Singapore

    V. Renugopalakrishnan

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  1. Pingzuo Li
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Li, P., Anumanthan, A., Gao, XG. et al. Expression of Recombinant Proteins in Pichia Pastoris . Appl Biochem Biotechnol 142, 105–124 (2007). https://doi.org/10.1007/s12010-007-0003-x

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