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Heterologous Expression of Transaldolase Gene Tal from Saccharomyces cerevisiae in Fusarium oxysporum for Enhanced Bioethanol Production

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Abstract

The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 g g−1 of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.

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Acknowledgments

This research was funded by National 863 Research Program (2006AA10Z424) and National Key Technology R&D Program of China during the eleventh Five-Year Plan period (2006BAD07A01).

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Authors and Affiliations

  1. Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China

    Jin-Xia Fan, Xiao-Xue Yang, Jin-Zhu Song, Xiao-Mei Huang, Zhong-Xiang Cheng, Lin Yao, Olivia S. Juba, Qing Liang, Qian Yang, Margaret Odeph, Yan Sun & Yun Wang

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  1. Jin-Xia Fan
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Correspondence to Jin-Zhu Song or Qian Yang.

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Fan, JX., Yang, XX., Song, JZ. et al. Heterologous Expression of Transaldolase Gene Tal from Saccharomyces cerevisiae in Fusarium oxysporum for Enhanced Bioethanol Production. Appl Biochem Biotechnol 164, 1023–1036 (2011). https://doi.org/10.1007/s12010-011-9191-5

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