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The Arxula adeninivoransATAL gene encoding transaldolase-gene characterization and biotechnological exploitation

  • Applied Genetics and Molecular Biotechnology
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Abstract

The yeast Arxulaadeninivorans provides an attractive expression platform and can be exploited as gene source for biotechnologically interesting proteins. In the following study, a striking example for the combination of both aspects is presented. The transaldolase-encoding A. adeninivorans ATAL gene, including its promoter and terminator elements, was isolated and characterized. The gene includes a coding sequence of 963 bp encoding a putative 321 amino acid protein of 35.0 kDa. The enzyme characteristics analyzed from isolates of native strains and recombinant strains overexpressing the ATAL gene revealed a molecular mass of ca. 140 kDa corresponding to a tetrameric structure, a pH optimum of ca. 5.5, and a temperature optimum of 20°C. The preferred substrates for the enzyme include d-erythrose-4-phosphate and d-fructose-6-phosphate, whereas d-glyceraldehyde is not converted. The ATAL expression level under salt-free conditions was observed to increase in media supplemented with 5% NaCl rendering the ATAL promoter attractive for moderate heterologous gene expression under high-salt conditions. Its suitability was assessed for the expression of a human serum albumin (HSA) reporter gene.

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Acknowledgements

We are grateful to Dr. I. Kunze for the helpful discussions and critical reading of the manuscript. We also thank I. Schmeling and H. Bohlmann for their excellent technical assistance. The research work was supported by grants from the “Ministry of Science and Research,” Sachsen/Anhalt (grant nos. 2067A/0025, 2463A/0086G), “Ministry of Economics,” Nordrhein-Westfalen (TPW-9910v08), and by Funds of Chemical Industry (GK).

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  1. Ayman El Fiki & Gamal El Metabteb

    Present address: National Center for Radiation Research and Technology, 3 Ahmed El-Zumor St., P.O. Box 29, Nasr City, Cairo, Egypt

Authors and Affiliations

  1. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466, Gatersleben, Germany

    Ayman El Fiki, Gamal El Metabteb, Carmen Bellebna, Thomas Wartmann & Gotthard Kunze

  2. Institut für Mikrobiologie, Universität Greifswald, Jahnstr. 15a, 17487, Greifswald, Germany

    Rüdiger Bode

  3. PharmedArtis Pharmaceuticals GmbH, Forckenbeckstr. 6, 52074, Aachen, Germany

    Gerd Gellissen

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  1. Ayman El Fiki
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  2. Gamal El Metabteb
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  3. Carmen Bellebna
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  7. Gotthard Kunze
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Correspondence to Gotthard Kunze.

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Fiki, A.E., Metabteb, G.E., Bellebna, C. et al. The Arxula adeninivoransATAL gene encoding transaldolase-gene characterization and biotechnological exploitation. Appl Microbiol Biotechnol 74, 1292–1299 (2007). https://doi.org/10.1007/s00253-006-0785-8

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  • DOI: https://doi.org/10.1007/s00253-006-0785-8

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