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Factors influencing somatic embryo maturation, high frequency germination and plantlet formation in Terminalia chebula Retz.

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Abstract

The factors influencing somatic embryo maturation, high frequency somatic embryo germination, and plantlet formation were studied in Terminalia chebula Retz. Maturation of somatic embryo were influenced by a number of factors such as in vitro culture passage, concentrations of sucrose, levels of abscisic acid (ABA), basal media and media additive combinations. Maximum frequency of somatic embryo maturation (57.22 ± 2.02), was obtained on MS medium supplemented with 50 g/l sucrose. Different factors such as strengths of MS nutrients, plant growth regulators, media additives and their combinations controlling somatic embryo germination and plantlet formation were studied. High frequency of germination and plantlet formation (58.80 ± 1.47) were achieved by subsequent subculture of mature somatic embryos on MS medium containing 30 g/l sucrose and 0.5 mg/l benzyladenine (BA). However, although duration of in vitro passage of the callus tissue was critical, contribution of the combinations of plant growth regulators and media additives showed nugatory effect on somatic embryo maturation and germination as evident from variable responses.

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Abbreviations

AC:

Activated charcoal

ABA:

Abscisic acid

BA:

Benzyladenine

2,4-D:

2,4 Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

KN:

Kinetin

NAA:

Napthalene acetic acid

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Acknowledgments

The authors would like to thank the Andhra Pradesh Netherlands Biotechnology Programme of The Netherlands Ministry for Development Co-operation for the financial support. C. Anjaneyulu would like to thank CSIR, New Delhi for the award of a Senior Research Fellowship.

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  1. Centre for Plant Molecular Biology (CPMB), Department of Genetics and Biotechnology, Osmania University, Hyderabad, Andhra Pradesh, 500007, India

    C. Anjaneyulu & C. C. Giri

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  1. C. Anjaneyulu
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  2. C. C. Giri
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Correspondence to C. C. Giri.

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Anjaneyulu, C., Giri, C.C. Factors influencing somatic embryo maturation, high frequency germination and plantlet formation in Terminalia chebula Retz.. Plant Biotechnol Rep 2, 153–161 (2008). https://doi.org/10.1007/s11816-008-0054-7

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  • DOI: https://doi.org/10.1007/s11816-008-0054-7

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