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Effect of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos

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Abstract

Knowledge of the relationship between indole-3-acetic acid (IAA) and abscisic acid (ABA) is relevant to control the development and the maturation of cork oak (Quercus suber L.) somatic embryos. The addition of 1 μM ABA to the culture medium significantly promoted somatic embryo maturation and increased both fresh and dry matter without affecting the relative water content. This effect was parallel to the pattern of variation observed in the endogenous ABA level, which increased from the immature to the mature stage. Endogenous ABA content during the occurrence of secondary embryogenesis was similar to that of the immature stage, showing that embryos with lower ABA levels produced secondary embryos. In contrast, IAA showed the highest concentration during early embryo development and decreased afterwards. Only in somatic embryos subjected to 1-week desiccation followed by stratification at 4 °C for 2 weeks, was a moderate increment of endogenous IAA content observed. IAA and ABA showed opposite levels during the development and maturation of cork oak somatic embryos and characterised specific stages of the embryonic development.

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Abbreviations

ABA:

abscisic acid

ACN:

acetonitrile

DW:

dry weight

FW:

fresh weight

IAA:

indole-3-acetic acid

MS:

Murashige and Skoog medium

RWC:

relative water content

SH:

Schenk and Hildebrandt medium

TW:

turgid weight

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Author notes

  1. G. García-Martín

    Present address: Dirección General de Investigación, Consejería de Educación, Comunidad de Madrid, C/Alcalá 32, 3a planta, Madrid, 28014, Spain

Authors and Affiliations

  1. IMIA, El Encín, Apartado 127, Alcalá de Henares, 28800, Spain

    G. García-Martín

  2. Departamento de Silvopascicultura, E.T.S.I. Montes, Universidad Politécnica de Madrid, Ciudad Universitaria, Madrid, 28040, Spain

    J.A. Manzanera

  3. Departamento de Biología Vegetal, Escuela Universitaria de Ingeniería Técnica Agrícola, Universidad Politécnica de Madrid, Ciudad Universitaria, Madrid, 28040, Spain

    M.E. González-Benito

Authors
  1. G. García-Martín
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  2. J.A. Manzanera
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  3. M.E. González-Benito
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Correspondence to J.A. Manzanera.

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García-Martín, G., Manzanera, J. & González-Benito, M. Effect of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos. Plant Cell Tiss Organ Cult 80, 171–177 (2005). https://doi.org/10.1007/s11240-004-1056-y

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  • DOI: https://doi.org/10.1007/s11240-004-1056-y

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