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Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora

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Abstract

A protocol was developed for short-term preservation and distribution of the plantation eucalypt, Corymbia torelliana × C. citriodora, using alginate-encapsulated shoot tips and nodes as synthetic seeds. Effects of sowing medium, auxin concentration, storage temperature and planting substrate on shoot regrowth or conversion into plantlets were assessed for four different clones. High frequencies of shoot regrowth (76–100%) from encapsulated explants were consistently obtained in hormone-free half- and full-strength Murashige and Skoog (MS) sowing media. Conversion into plantlets from synthetic seeds was achieved on half-strength MS medium by treating shoot tips or nodes with 4.9–78.4 μM IBA prior to encapsulation. Pre-treatment with 19.6 μM IBA provided 62–100% conversion, and 95–100% of plantlets survived after acclimatisation under nursery conditions. Synthetic seeds containing explants pre-treated with IBA were stored for 8 weeks much more effectively at 25°C than at 4°C, with regrowth frequencies of 50–84% at 25°C compared with 0–4% at 4°C. To eliminate the in vitro culture step after encapsulation, synthetic seeds were allowed to pre-convert before sowing directly onto a range of ex vitro non-sterile planting substrates. Highest frequencies (46–90%) of plantlet formation from pre-converted synthetic seeds were obtained by transferring shoot tip-derived synthetic seeds onto an organic compost substrate. These plantlets exhibited almost 100% survival in the nursery without mist irrigation. Pre-conversion of non-embryonic synthetic seeds is a novel technique that provides a convenient alternative to somatic embryo-derived artificial seeds.

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Abbreviations

ANOVA:

Analysis of variance

BA:

6-benzyladenine

IBA:

Indole-3-butyric acid

IVS:

In vitro soil-less

MS:

Murashige and Skoog

NAA:

α-Naphthalene acetic acid

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Acknowledgments

We thank David Lee (Agri-Science Queensland) for providing seeds and Tracey McMahon for useful discussions and critical reading of the manuscript. This project was funded by the Queensland National and International Research Alliances Program and supported by a postgraduate scholarship to CDH from the Vietnam Ministry of Education and Training.

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Authors and Affiliations

  1. School of Science and Education, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia

    Cao Dinh Hung

  2. Agri-Science Queensland, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia

    Stephen J. Trueman

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  1. Cao Dinh Hung
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  2. Stephen J. Trueman
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Correspondence to Cao Dinh Hung.

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Communicated by M. Horbowicz.

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Hung, C.D., Trueman, S.J. Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora . Acta Physiol Plant 34, 117–128 (2012). https://doi.org/10.1007/s11738-011-0810-0

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