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Control of in vitro rooting and plant development in Corymbia maculata by silver nitrate, silver thiosulfate and thiosulfate ion

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Abstract

Plant regeneration and transformation in vitro is often improved by adding silver ion (Ag+) to the culture media as AgNO3 or silver thiosulfate (STS). Ag+ reacts with substances to form insoluble precipitates, while thiosulfate (S2O3 2−) interferes with these reactions. We studied the implications of silver precipitation and S2O3 2− in the medium for culture development by (1) examining formation of Ag+ precipitates from AgNO3 versus STS in agar gels and their possible dependence on agar type; (2) comparing Corymbia maculata culture responses to AgNO3 and STS and determining which better suits control of culture development; (3) clarifying whether STS-dependent alterations in culture development are due to Ag+ alone or also to a separate influence of S2O3 2−. Silver precipitates appeared in aqueous gels of four agar brands supplemented with AgNO3, but not in Phytagel™, which remained transparent. No precipitation was observed in gels with STS. Indole-3-butyric acid (IBA)-mediated adventitious root induction and shoot growth were higher in C. maculata shoot tips cultured on gels with STS versus AgNO3 (6–25 μM Ag+). IBA-treated shoot tips exhibited enhanced adventitious root regeneration, accelerated root elongation, increased frequency of lateral root formation, and stimulated shoot growth mediated by 100–250 μM sodium thiosulfate (Na2S2O3) in medium without Ag+. The potency of S2O3 2− in facilitating culture development has never been recognized. It is inferred that superiority of STS in stimulating multiple responses of C. maculata culture results from sustained biological activity of Ag+ through prevention of its precipitation, and from impact of S2O3 2− on cell differentiation and growth.

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Abbreviations

BA:

6-Benzyladenine

IBA:

Indole-3-butyric acid

MES:

2-(N-morpholino) ethanesulphonic acid

MS:

Murashige and Skoog medium

NAA:

α-naphthalene acetic acid

RIM:

Root induction medium

REM:

Root elongation medium

STS:

Silver thiosulfate

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Acknowledgments

This article is dedicated to the memory of Dr. Aaron Zelcer (1943–2010). The research was supported in part by the Forestry Board (Grant No. 277-0212-07), and is contribution No. 117/2010 from the ARO, The Volcani Center, Bet Dagan, Israel.

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Authors and Affiliations

  1. Department of Vegetable Research, Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

    Benjamin Steinitz, Nurit Barr & Yona Tabib

  2. Department of Agronomy and Natural Resources, Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

    Yiftach Vaknin

  3. Department of Environmental Physics and Irrigation, Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, 50250, Bet Dagan, Israel

    Nirit Bernstein

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  1. Benjamin Steinitz
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  2. Nurit Barr
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  3. Yona Tabib
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  4. Yiftach Vaknin
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  5. Nirit Bernstein
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Correspondence to Benjamin Steinitz.

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Communicated by K. Kamo.

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Steinitz, B., Barr, N., Tabib, Y. et al. Control of in vitro rooting and plant development in Corymbia maculata by silver nitrate, silver thiosulfate and thiosulfate ion. Plant Cell Rep 29, 1315–1323 (2010). https://doi.org/10.1007/s00299-010-0918-5

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  • DOI: https://doi.org/10.1007/s00299-010-0918-5

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