Summary
In vitro recalcitrance is the inability of plant cells, tissues and organs to respond to tissue culture manipulations. With respect to plant regeneration, recalcitrance can be a major limiting factor for the biotechnological exploitation of economically important plant species and it can also impair the wider application of in vitro conservation techniques. This first paper introduces a compilation of Symposium papers, collectively entitled “Do we understand in vitro plant recalcitrance?”, presented at the 1999 Congress of the Society for In Vitro Biology. The Symposium reviewed recalcitrance in the context of genetic predeterminism, molecular markers and gene expression patterns, whole and explant physiology, stress physiology, habituation, neoplastic progression and plant cancer. The symposium contributors present fundamental and applied investigative approaches which have the potential to enhance our current understanding of in vitro recalcitrance and to assist in overcoming the problems associated with nonresponsive plant cultures. This introductory paper presents the general concept of recalcitrance in relation to whole-plant and explant physiology and considers basic aspects of tissue culture manipulations in the context of recalcitrance problems.
References
Abdullah, R.; Cocking, E. C.; Thompson, J. A. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090; 1986.
Anthony, J. M.; Senaratna, T.; Dixon, K. W.; Sivasithamparam, K.; Bunn, E. In vitro regeneneration of recalcitrant Australian plants. In Vitro Cell. Dev. Biol. 35:53-A; P-1062; 1999.
Benson, E. E. Do free radicals have a role in plant tissue culture recalcitrance? In Vitro Cell. Dev. Biol.-Plant 36:163–170; 2000.
Beruto, M.; Beruto, D.; Debergh, P. Influence of agar on in vitro cultures. I. Physicochemical properties of agar and agar gelled media. In Vitro Cell. Dev. Biol. Plant 35:86–93; 1999a.
Beruto, M.; Beruto, D.; Debergh, P. Influence of agar on in vitro cultures. II. Biological performance of Ranunculus on media solidified with three different agar brands. In Vitro Cell. Dev. Biol.-Plant 35:93–101; 1999b.
Bhat, S. R.; Chandel, K. P. S. A novel technique to overcome browning in tissue culture. Plant Cell Rep. 10:358–361; 1991.
Blackhall, N. W.; Jotham, J. P.; Azhakanandan, K.; Power, J. B.; Lowe, K. C.; Cocking, E. C.; Davey, M. R. Callus initiation, maintenance and shoot induction in rice. In: Hall, R. D., ed. Plant cell culture protocols (Methods in molecular biology Vol. III). Totowa, NJ: Humana Press Inc.; 1999; 19–29.
Bonga, J. M.; Von Aderkas, P. In vitro culture of trees. Dordrecht. The Netherlands: Kluwer Academic Publishers; 1992.
Chevereau, E.; Morgues, F.; Nevue, M.; Chevalier, M. Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear. In Vitro Cell. Dev. Biol.-Plant 33:173–179; 1997.
Cairney, J.; Xu, N.; PacKay, J.; Pullman, J. Transcript profiling: a tool to assess the development of conifer embryos. In Vitro Cell. Dev. Biol.-Plant 36:155–162; 2000.
Cairney, J.; Xu, N.; Pullman, G. S.; Ciavatta, V. T.; Johns, B. Natural and somatic embryo development in loblolly pine: gene expression studies using differential display and DNA arrays. Appl. Biochem. Biotechnol. 77–79:5–17; 1999.
Demeester, J. J.; Matthijs, D. G.; Pascat, B.; Debergh, P. C. Toward a controllable headspace composition—growth, development and headspace of micropropagated Prunus rootstock in different containers. In Vitro Cell. Dev. Biol.-Plant 31:105–112; 1995.
Devi Prasad, P. V.; Potluri, S. D. P. Influence of proline and hydroxyproline on salt-stressed axillary bud cultures of two varieties of potato (Solanum tuberosum). In Vitro Cell. Dev. Biol.-Plant 32:47–50; 1996.
D'Onfrio, C.; Morini, S.; Bellocchi, G. Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tiss. Org. Cult. 53:91–98; 1998.
Edson, J. L.; Leege-Brusven, A. D.; Everett, R. L.; Wenny, D. L. Minimising growth regulators in shoot culture of an endangered plant, Hackelia venusta (Boraginaceae). In Vitro Cell. Dev. Biol.-Plant 32:267–271; 1996.
Engelmann, F. In vitro conservation of tropical plant germplasm—a review. Euphytica 57:227–243; 1991.
Ewald, D. Advances in tissue culture of adult larch. In Vitro Cell. Dev. Biol.-Plant 34:325–330; 1998.
Faruque, M. O.; Farzana, T.; Seraj, Z. I.; Sarker, R. H.; Khatum, A. A. Variations in green plant regeneration responses from anthers of indica rice and their hybrids with japonica cv Taipei 309. Plant Cell Tiss. Org. Cult. 54:191–195; 1998.
Faure, O.; Diemer, F.; Moja, S.; Jullien, F. Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks. Plant Cell Tiss. Org. Cult. 52:209–212; 1998.
Fay, M. F. Conservation of rare and endangered plants using in vitro methods. In Vitro Cell. Dev. Biol. 28P:1–4; 1992.
Franck-Duchenne, M.; Wang, Y.; Ben Tahar, S.; Beachy, R. N. In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide. Plant Cell Tiss. Org. Cult. 53:79–84; 1998.
Fridborg, G.; Pedersen, M.; Landström, L-E.; Eriksson, T. The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol. Plant 43:104–106; 1978.
Gaspar, Th.; Kevers, C.; Bisbis, B.; Franck, Th.; Crevecoeur, M.; Greppin, H.; Dommes, J. Loss of plant organogenic totipotency in the course of neoplastic progression. In Vitro Cell. Dev. Biol.-Plant 36:171–181; 2000.
Gaspar, Th.; Kevers, C.; Penel, C.; Greppin, H.; Reid, D. M.; Thorpe, T. A. Plant growth regulators in plant tissue culture. In Vitro Cell. Dev. Biol.-Plant 32:272–289; 1996.
González-Benito, M. E.; Martin, C.; Iriondo, J. M.; Pérez, C. Conservation of the rate and endangered plants endemic to Spain. In: Benson, E. E., ed. Plant conservation biotechnology. London, UK: Taylor and Francis; 1999; 251–264.
Gude, H.; van der Plas, L. H. W. Endogenous ethylene formation and the development of the alternative pathway in potato tuber disks. Physiol. Plant 65:57–62; 1985.
Guohua, M. Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants. Plant Cell Tiss. Org. Cult. 54:1–7; 1998.
Hall, R. D. Plant cell culture protocols (Methods in molecular biology, Vol. III). Totowa, NJ: Humana Press; 1999.
Harding, K. Approaches to assess the genetic stability of plants recovered from in vitro cultures. In: Normah, M. N.; Narimah, M. K.; Clyde, M. M., ed. In vitro conservation of plant genetic resources. Kuala Lumpur, Malaysia: Plant Biotechnology Laboratory UKM; 1996; 135–168.
Heatley, M. E.; Smith, R. H. Whole plant regeneration from the shoot apex of Arachis hypogaca L. In Vitro Cell. Dev. Biol.-Plant 32:115–118; 1996.
Hutchinson, M. J.; Murr, D.; Krishnaraj, S.; Senaratna, T.; Saxena, P. K. Does ethylene play a role in thidiazuron-regulated somatic hypocotyl cultures? In Vitro Cell. Dev. Biol.-Plant 33:136–141; 1997.
Hsia, C-N.; Korban, S. S. Factors affecting in vitro establishment and shoot proliferation of Rosa hybrida L. and Rosa chinensis minima. In Vitro Cell. Dev. Biol.-Plant 32:217–222; 1996.
Hyde, C. L.; Phillips, G. C. Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell. Dev. Biol.-Plant 32:72–80; 1996.
Jähne-Gartner, A.; Lörz, H. Protocols for anther and microscopore culture of barley. In: Hall, R. D., ed. Plant cell culture protocols (Methods in molecular biology, Vol. III). Totowa, NJ: Humana Press Inc.; 1999; 269–279.
Joy, R. W. IV; Patel, K. R.; Thorpe, T. A. Ascorbic acid enhancement of organogenesis in tobacco. Plant Cell Tiss. Org. Cult. 13:219–228; 1988.
Kevers, C.; Bisbis, B.; Le Dily, F.; Billard, J. P.; Huault, C.; Gaspar, Th. Darkness improves growth and delays necrosis in nonchlorophyllous habituated sugarbeet callus: biochemical changes. In Vitro Cell. Dev. Biol.-Plant 31:122–126; 1995.
Kiviharju, E.; Pehu, E. The effect of cold and heat pretreatments on anther culture responses of Avena sativa and A. sterilis. Plant Cell Tiss. Org. Cult. 54:97–104; 1998.
Klee, H. J.; Romano, C. P. The roles of phytohormones in development as studies in transgenic plants. Crit. Rev. Plant Sci. 13:311–324; 1994.
Kumar, P. P.; Lakshmanan, P.; Thorpe, T. A. Regulation of morphogenesis in plant tissue culture by ethylene. In Vitro Cell. Dev. Biol.-Plant 34:93–103; 1998.
Lardet, L.; Aguilar, M. E.; Michaux-Ferriere, N.; Berthouly, M. Effect of strictly plant-related factors on the responses of Hevea brasiliensis and Theobroma cacoa nodal explants cultured in vitro. In Vitro Cell. Dev. Biol.-Plant 34:34–40; 1998.
Li, X. Y.; Huang, F. H.; Gbur, E. E. Jr. Polyethylene glycol-promoted development of somatic embryos in loblolly pine (Pinus taeda L.). In Vitro Cell. Dev. Biol.-Plant 33:184–189; 1997.
Litz, R. E.; Chaves, V. M.; Moon, P. A. Induction of embryogenic cultures from mature-phase tropical and subtropical tress and control of somatic embryo maturation and germination. In: Bruns, S.; Mantell, S.; Trägardh, C.; Viana, A. M., ed. Recent advances in biotechnology for tree conservation and management. Stockholm, Sweden; International Foundation for Science; 1999; 232–243.
Litz, R. E.; Moon, P. A.; Chaves, V. M. Somatic embryogenesis from leaf calluses derived from mature trees of the cycad Ceratozamia hildae (Gymnospermae). Plant Cell Tiss. Org. Cult. 40:25–31; 1995.
Majada, J. P.; Fal, M. A.; Sanchez-Tames, R. The effect of ventilation rate on proliferation and hyperhydricity of Dianthus caryophyllus L. In Vitro Cell. Dev. Biol.-Plant 33:62–69; 1997.
Michler, C. H.; Lineberger, R. D. Effects of light on somatic embryo development and abscisic acid levels in carrot suspension cultures. Plant Cell. Tiss. Org. Cult. 11:189–207; 1987.
McCown, B. H. Recalcitrance of woody and herbaceous perennial plants: Dealing with genetic predeterminism. In Vitro Cell. Dev. Biol.-Plant 36:149–154; 2000.
Meier, K.; Reuther, G. Factor controlling micropropagation of mature Fagus sylvatica. Plant Cell Tiss. Org. Cult. 39:231–238; 1994.
Miguel, C. M.; Druart, P.; Oliveira, M. M. Shoot regeneration from adventitious buds induced by juvenile and adult almond (Prunus dulcis Mill.) explants. In Vitro Cell. Dev. Biol.-Plant 32:148–153; 1996.
Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron: a potent regulator of in vitro morphogenesis. In Vitro Cell. Dev. Biol.-Plant 34:267–275; 1998.
Nadel, B. L.; Altman, A.; Pleban, S. In vitro development of mature Fagus sylvatica buds II. Seasonal changes in response to plant growth regulators. J. Plant. Physiol. 138:136–141; 1991.
Ouedraogo, J. T.; St-Pierre, C. A.; Collin, J.; Rioux, S.; Comeau, A. Effect of amino acids, growth regulators and genotype on androgenesis in barley. Plant Cell Tiss. Org. Cult. 53:59–66; 1998.
Orchard, J. E.; Collin, H. A.; Hardwick, K. Biochemical and physiological aspects of leaf development in cocoa (Theobroma cacao). IV. Changes in growth inhibitors. Plant Sci. Lett. 18:299–305; 1980.
Parra, R.; Amo-Marco, J. B. Factors affecting in vitro shoot proliferation of Myrtus communis L.: A comparison of adult and seedling material. In Vitro Cell. Dev. Biol.-Plant 34:104–107; 1998.
Peng, M.; Ziauddin, A.; Wolwyn, D. J. Development of asparagus microscpores in vivo and in vitro is influenced by gametogenic growth stage and cold treatment. In Vitro Cell. Dev. Biol.-Plant 33:263–268; 1997.
Perata, P.; LoSchiavo, F.; Alpi, A. Ethanol production and toxicity in suspension-cultured carrot cells and embryos. Planta 173:322–329; 1988.
Pierik, R. L. M. In vitro culture of higher plants. Dordrecht, The Netherlands: Martinus Nijhoff Publishers; 1987.
Rao, A. M.; Padma Sree, K.; Kavi Kishor, P. B. Enhanced plant regeneration in grain and sweet sorghum by asparagine, proline and cefotaxime. Plant Cell Rep. 15:72–75; 1995.
Ravnikar, M.; Vilhar, B.; Gogala, N. Stimulatory effects of jasmonic acid on potato stem node and protoplast culture. J. Plant Growth Regul. 11:29–33; 1992.
Reed, B. M.; Chang, Y. Medium- and long-term storage of in vitro cultures of temperature and nut crops. In: Razdan, M. K.; Cocking, E. C., ed. General aspects (Conservation of plant genetic resources in vitro, Vol. 1). Enfield, NC: Science Publishers, Inc.; 1997:67–106.
Revilla, M. A.; Pacheco, J.; Casares, A.; Rodríguez, R. In vitro reinvigoration of olive trees (Olea europaea L.) through micrografting. In Vitro Cell. Dev. Biol.-Plant 32:257–261; 1996.
Rey, M.; Díaz-Sala, C.; Rodríguez, R. Exogenous polyamines improve rooting in hazel microshoots. Plant Cell Tiss. Org. Cult. 36:303–308; 1994.
Samoylov, V. M.; Tucker, D. M.; Parrott, W. A. Soybean [Glycine max (L.) Merrill] embryogenic cultures: The role of sucrose and total nitrogen content on proliferation. In Vitro Cell. Dev. Biol.-Plant 34:8–13; 1998.
Santarem, E. R.; Pelissier, B.; Finer, J. J. Effect of explant orientation, pH, solidifying agent and wounding on initiation of soybean somatic embryos. In Vitro Cell. Dev. Biol.-Plant 33:13–19; 1997.
Saunders, J. W.; Tsai, C. J. Production of somatic embryos and shoots from sugarbeet, callus: effects of abscisic acid, other growth regulators, nitrogen source, sucrose concentration and genotype. In Vitro Cell. Dev. Biol.-Plant 35:18–24; 1999.
Selby, C.; Harvey, B. M. R. The influence of natural and in vitro bud flushing on adventitious bud production in sitka spruce (Picea stichensis Bong.) bud and needle cultures. New Phytol. 100:549–562; 1985.
Selby, C.; McRoberts, W. C.; Hamilton, J. T. G.; Harvey, B. M. R. Inhibition of somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.] by butylated hydroxytoluene, a volatile antioxidant released by parafilm. Plant Cell Rep. 16:192–195; 1996.
Spomer, L. A.; Smith, M. A. L. Direct measurement of water availability in gelled plant tissue culture media. In Vitro Cell. Dev. Biol.-Plant 32:210–215; 1996.
Stanilova, M. I.; Ilcheva, V. P.; Zagorska, N. A. Morphogenetic potential and in vitro micropropagation of endangered plant species Leucojum aestivum L. and Lilium rhodopacum. Delip. Plant Cell Rep. 13:452–453; 1994.
Thompson, J. A.; Abdullah, R.; Cocking, E. C. Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose. Plant Sci. 47:123–133; 1986.
Thomas, P. Humid incubation period and plantlet age influence acclimatization and establishment of micropropagated grapes. In Vitro Cell. Dev. Biol.-Plant 34:52–56; 1998.
Vagera, J.; Havránek, P. Effect of minimization of culture medium on the process of androgenesis in vitro (Nicotiana tabacum L. var. White Burley, Datura innoxia, Mill.) Biochem. Physiol. Pflanz. 177:266–274; 1982.
Vasil, I. K. Developing cell and tissue culture systems for the improvement of cereal and grass crops. J. Plant Physiol. 128:193–218; 1987.
Yu, K.; Reed, B. M. Improved shoot multiplication of mature hazelnut (Corylus avellana L.) in vitro using glucose as a carbon source. Plant Cell Rep. 12:256–259; 1993.
Zimmerman, T. W.; Cobb, B. G. Vitrification and soluble carbohydrate levels in Petunia leaves as influenced by media, gelrite and sucrose concentration. Plant Cell Rep. 8:358–360; 1989.
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Benson, E.E. Sepecial symposium: In vitro plant recalcitrance in vitro plant recalcitrance: An introduction. In Vitro Cell.Dev.Biol.-Plant 36, 141–148 (2000). https://doi.org/10.1007/s11627-000-0029-z
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DOI: https://doi.org/10.1007/s11627-000-0029-z