Abstract
The diet of organisms generally provides a sufficient supply of energy and building materials for healthy growth and development, but should also contain essential nutrients. Species differ in their exogenous requirements, but it is not clear why some species are able to synthesize essential nutrients, while others are not. The unsaturated fatty acid, linoleic acid (LA; 18:2n-6) plays an important role in functions such as cell physiology, immunity, and reproduction, and is an essential nutrient in diverse organisms. LA is readily synthesized in bacteria, protozoa and plants, but it was long thought that all animals lacked the ability to synthesize LA de novo and thus required a dietary source of this fatty acid. Over the years, however, an increasing number of studies have shown active LA synthesis in animals, including insects, nematodes and pulmonates. Despite continued interest in LA metabolism, it has remained unclear why some organisms can synthesize LA while others cannot. Here, we review the mechanisms by which LA is synthesized and which biological functions LA supports in different organisms to answer the question why LA synthesis was lost and repeatedly gained during the evolution of distinct invertebrate groups. We propose several hypotheses and compile data from the available literature to identify which factors promote LA synthesis within a phylogenetic framework. We have not found a clear link between our proposed hypotheses and LA synthesis; therefore we suggest that LA synthesis may be facilitated through bifunctionality of desaturase enzymes or evolved through a combination of different selective pressures.
Similar content being viewed by others
Introduction
Among all the nutritional compounds that make up an organism’s body, many are required as a nutritional supplement, i.e. organisms cannot synthesize these metabolites themselves. Lack of an external resource for such essential molecules can cause a range of deficiency diseases, for example in the case of vitamin, amino acid or mineral deficiencies (Anderson and Connor 1989; Brock and Chapple 2016). The exogenous requirement for essential nutrients is, however, not universal among organisms. Some taxonomic groups, such as microorganisms and plants, are to a large extent autotrophic and can synthesize vitamins, amino acids, and lipids de novo from simpler compounds. Metazoan species, in contrast, are generally heterotrophic for many metabolites, as they lack key enzymes of essential metabolic pathways (Ellers et al. 2012). Metazoans thus generally need regular dietary intake of essential micronutrients to prevent deficiency diseases. It remains to be resolved, however, why some species are able to synthesize essential nutrients, while others are not. One possible explanation is that for some species essential nutrients are abundantly available in their environment. High consumption of essential nutrients may relax selective pressures on an organism to maintain autotrophy for such metabolites (Pandey et al. 2015). Furthermore, if nutrients are supplied by a symbiotic partner or are present abundantly in the environment, de novo synthesis of the nutrient in question becomes redundant and prone to loss or degradation (Visser and Ellers 2008; Ellers et al. 2012; Helliwell et al. 2013). The presence of excess amounts of vitamin C in the diet of primates and other mammals, for example, is thought to have rendered de novo synthesis obsolete and mutation accumulation was found to compromise functioning of the gene underlying vitamin C production (Chatterjee 1973; Ohta and Nishikimi 1999). Similarly, some genes required for the biosynthesis of vitamin B6 were lost after the divergence of vertebrates and invertebrates, leaving all mammals unable to synthesize vitamin B6 (Kennedy 2016).
One class of essential nutrients is found among polyunsaturated fatty acids (PUFAs). PUFAs are long chain fatty acids with multiple double bonds that are vital for body functions, such as the formation and functioning of cell membranes, as well as the immune system. PUFAs further play an important role in cell physiology, signaling and reproduction (Belury 2002; El-Yassimi et al. 2008; De Veth et al. 2009), and in humans PUFAs lower the risk of coronary artery disease, some nerve diseases like Alzheimer’s, schizophrenia and metabolic syndrome (Booth-Kewley and Friedman 1987; Kalmijn et al. 1997; Horrobin 1998; Hulbert et al. 2005). Some PUFAs have been recognized in animals as essential nutrients for which a dietary source is required (Sinclair et al. 2007; Grosso et al. 2016). The critical step in PUFA biosynthesis is the introduction of a second double bond in a mono-unsaturated fatty acid leading to the formation of linoleic acid (LA). This enzymatic step is carried out by specific desaturases, which were previously thought to be restricted to bacteria, protozoa and plants (de Renobales et al. 1986). Louloudes et al. (1961) were one of the first to show that an insect, the American cockroach Periplaneta americana, was able to biosynthesize LA de novo. Since that time, a growing body of research on LA and other PUFAs has confirmed that LA can be synthesized by a range of different insects (de Renobales et al. 1986; Cripps et al. 1986; Aboshi et al. 2013; Shimizu et al. 2014) and other invertebrates, including nematodes and pulmonates (Weinert et al. 1993; Wallis et al. 2002). Consequently, these findings have challenged the long-held assumption that animals lack the ability to synthesize LA de novo.
We currently have a poor understanding of the evolutionary dynamics of the ability for LA synthesis. Until now LA synthesis is not commonly found, but species that are able to produce LA are found in a diverse set of taxonomic groups. A phylogenetic analysis of the pattern of presence and absence of biosynthetic ability of LA is therefore needed to distinguish between different potential evolutionary scenarios. One hypothesis is that a conserved loss of LA synthesis early in the evolution of the Animalia was followed by multiple independent reversals of the trait. Alternatively, the ancestral trait of LA synthesis may have been retained across most families, but many independent losses of the trait were incurred in more shallow evolutionary branches. Clearly, either of these scenarios will involve multiple evolutionary transitions, which suggests that LA biosynthesis is an evolutionarily labile trait. The question is which genetic pathways enable this high evolvability and what convergent selection pressures are leading to this repeated pattern of (re)gain and/or loss. In this review we develop a new perspective on the evolutionary dynamics of LA synthesis. We will (1) compile known cases of LA synthetic ability across the animal kingdom and use a phylogenetic approach to explore macro-evolutionary patterns; (2) review the biochemical and genetic pathways involved in LA synthesis to compare LA synthesis mechanisms in animals to that in plants and microbes; and (3) present new prospects on the biological function of LA synthesis, including potential selective forces leading to gains or losses of the ability to produce LA.
LA Synthesis Ability and Evolutionary Transitions in Animals
To gain more insight into the evolution of LA synthesis, we have compiled all available information on the ability to produce LA for invertebrates and reconstructed their phylogeny (Fig. 1; Supplementary information 1). Until now, 54 invertebrate species have been tested for their ability to synthesize LA and over 40% of these species were found capable of producing LA de novo (Fig. 1; Table 1). Within the primitive invertebrates Crustacea, Nematoda and Acari, all seven species tested were found to synthesize LA, revealing that the more ancestral invertebrate groups have functional LA synthetic pathways. The ability for LA synthesis was absent in more primitive insect groups, including Zygentoma, Ephemeroptera, Odonata, Dermaptera, and Plecoptera, although only few species within these groups were tested (5 species in total). Of all insects tested, almost 60% of species were found incapable of producing LA de novo. The other 40% did produce LA, but LA synthesis ability seems to be scattered across the different insect orders.
A phylogenetic tree reconstructed based on molecular and morphological data (see Supplementary material 2) of species able (red branches) and unable (black branches) to synthesize LA de novo. (Color figure online)
Character tracing of LA synthesis in insects suggested that the ability for de novo synthesis in 18 species is a secondarily derived character rather than an ancestral trait (Fig. 1), particularly because LA synthesis is absent from the primitive invertebrate groups. If we assume absence of LA synthetic ability to be the ancestral state in the insects, as a result of convergent evolution (homoplasy) LA synthesis could have evolved eight times independently in different lineages, and lost in two insect groups (Lepidoptera, Diptera). Overall, with the exception of flies and butterflies, the ability for LA synthesis re-evolved in all major insect orders. In Blattodea and Hemiptera the evolutionary history of LA synthesis is equivocal. For the cockroaches, LA synthesis may have been regained once in the common ancestor of Periplaneta sp. and Eurycotis floridana and subsequently lost again in Blatta orientalis. Alternatively, LA synthesis was regained on two separate occasions, once in E. floridana and once in the common ancestor of Periplaneta sp. The same holds true for the hemipteran clade where LA synthesis could have been regained on two separate occasions or was regained once and subsequently lost again in some species. There is clear support for the hypothesis that a conserved loss of LA synthesis early in the evolution of the Animalia was followed by multiple independent reversals of the trait. To better comprehend those evolutionary transitions, we should look into the biochemical and genetic pathways underlying LA synthesis in these species.
Biochemical and Genetic Pathways Underlying LA Synthesis
Fatty acid desaturases are enzymes that introduce a double bond between carbon atoms and remove a hydrogen atom, thereby creating unsaturated fatty acids (FAs) (Fig. 2). Three types of desaturases are involved in placing double bonds in long chain FAs: acyl-lipid, acyl-ACP and acyl-CoA desaturases (Fig. 3; Murata and Wada 1995). Acyl-lipid desaturases are found in cyanobacteria and some plants, where bonds are introduced into fatty acids that are in a lipid-bound form (Murata and Wada 1995; Los and Murata 1998). Acyl-ACP desaturases are present in plant plastids and introduce double bonds to lipids bound to an acyl carrier protein (ACP) (Murata and Wada 1995). Acyl-CoA desaturases are found in fungal and animal cells in which double bonds are introduced involving coenzyme A (CoA) (Macartney et al. 1994). PUFAs can be synthesized through the action of a range of desaturases, generating different products depending on the location at which double bonds are introduced in a long chain FA molecule (Wallis et al. 2002).
A schematic overview of the fatty acid desaturation mechanism
Three types of desaturases are involved in introducing double bonds to long chain FAs with their final products in plants, microorganisms and animals: acyl-ACP, acyl-lipid and acyl-CoA desaturases, respectively (Murata and Wada 1995). Each double bond is indicated by Δx or by the number in brackets, where the double bond is located on the xth carbon–carbon bond, counting from the carboxylic acid end. For example in microorganisms, oleic acid, C18:1 (9) already contains a bond at the 9th carbon position and a bond at the 12th carbon position is added to form linoleic acid, C18:2 (9, 12)
LA is synthesized from oleic acid (C18:1), a long chain FA that already contains a double bond between the 9th and 10th carbon atom. Using oleic acid as a substrate, a ∆12 desaturase then introduces a second double bond between the 12th and 13th C atom (Fig. 3). In plants and yeasts genes encoding a ∆12 desaturase have been identified and characterized, for example in Kluyveromyces lactis (Kainou et al. 2006), Candida parapsilosis (Buček et al. 2014), and Arabidopsis thaliana (Covello and Reed 1996). In plants LA desaturation has been linked to six loci (fad &, fad 2, fad 4, fad 5, fad 6 and fad 7) (Browse and Somerville 1991) and it was discovered that the enzyme encoding plant ∆12 desaturase contains three conservative histidine boxes belonging to the Omega family (Hashimoto et al. 2008). Until now, orthologs of these genes seem to be missing completely in most animals (except for Caenorhabditis elegans, see below), also in species that were found to synthesize LA (Watts and Browse 2002; Alonso et al. 2003, 2014; Blaul et al. 2014). This confirms the result from our phylogenetic analysis that LA synthesis in animals evolved secondarily, and suggests that a different genetic pathway is enabling LA synthesis in animals.
Whilst 25 arthropod species were found capable of synthesizing LA (Fig. 1), for only very few of those species a ∆12 desaturase gene or enzyme has so far been identified (Zhou et al. 2008).
Caenorhabditis elegans is the exception, because a plant-like ∆12 desaturase gene has been found in this species (Peyou-Ndi et al. 2000). The enzyme encoded by the gene, however, was discovered to have an alternative mechanism of desaturase functioning which relies on bifunctional activity of ∆12/∆15 desaturases (Zhou et al. 2011). Similarly, the ∆12/∆15 bifunctional desaturase was also found in the soil yeast Lipomyces kononenkoae, which uses a novel gene derived from gene duplication (Yan et al. 2013). However, the multiple genetic pathways that underlie LA synthesis in fungi and nematodes are unlikely to share a common evolutionary origin since a comparative phylogenetic analysis suggested that nematode Acyl-CoA desaturases probably arose independently from those found in fungi and protozoa (Zhou et al. 2011). Biochemical and genetic pathways of linoleic acid in plants and microbes are extensively reviewed in other publications (Farmer 1994; Certik and Shimizu 1999; Thelen and Ohlrogge 2002; Weber 2002; Qi et al. 2004; Sampath and Ntambi 2005; Palmquist et al. 2005; Jenkins et al. 2014).
In insects capable of LA synthesis, only two genes have so far been identified that encode a Δ12-desaturase; one in the red flour beetle, Tribolium castaneum and another one in the house cricket, Acheta domestiucus (Zhou et al. 2008). A comparative analysis showed that their Δ12-desaturase genes are more closely related to the archetypal Δ9-desaturase from rats than to Δ12-desaturases widely reported in plants (Zhou et al. 2008, 2011; Yan et al. 2013) and they most likely evolved independently from plant, fungal and nematode Δ12-desaturases. Although both species share the common ancestor of the insects, their Δ12-desaturase genes do not cluster together but they are more related to Δ9-desaturases genes of their own species, again suggesting evolution by an independent route (Zhou et al. 2008). The question arises if the close similarity to Δ9-desaturases genes is indicative of ∆9/∆12 desaturase bifunctionality similar to what has been found in C. elegans and some fungi. As of yet no bifunctional activity has, however, been confirmed (Zhou et al. 2008). The similarity in histidine motifs between ∆9 and ∆12 desaturases frustrates attempts to identify ∆12-desaturase genes in more species. For example, gene identification was attempted in the parasitoid Nasonia vitripennis, but despite several candidates no ∆12 functionality was detected in any of the genes (Blaul et al. 2014). As gene detection can be compromised by dual functionality of ∆9/∆12-desaturases (Zhou et al. 2011) and by the high similarity between ∆9 and ∆12 desaturase motifs (Zhou et al. 2008, 2011), future research should focus on those two scenarios.
Acquisition and Utilization of LA as Drivers of LA Synthetic Ability
Tracing the evolutionary history of a trait can reveal major evolutionary trait changes, such as gains, losses and reversions, but we need to link these transitions to the ecology of the organism to provide adaptive explanations for trait evolution. The selective forces on LA biosynthetic ability are expected to depend on the acquisition of LA from external sources and on the magnitude of LA utilization by the organism. We, therefore, need to address two key questions: (1) what are the ways in which an organism can obtain LA, and (2) to what extent does an organism utilize LA (Fig. 4)?
A schematic overview of the means by which LA can be acquired and utilized by organisms
Acquisition of LA from the Environment
Diet was proposed as a key ecological factor affecting LA synthesis (Cripps et al. 1986) with the evolutionary loss of LA synthesis resulting from environmental compensation (Visser et al. 2010; Ellers et al. 2012). A scenario under which the common ancestor of (primitive) insects fed on a diet rich in LA (i.e. herbivores/omnivores) could have fueled the evolutionary loss of LA synthetic ability, as the trait would have become unnecessary. Conversely, LA may be required by species that feed on a diet containing little or no LA (carnivores), leading to a regain of LA synthetic ability in these lineages (Stanley-Samuelson et al. 1991). The evolution of LA synthesis seems unrelated to dietary preferences of distinct invertebrate groups, because 50% of species capable of de novo LA synthesis are herbivores and 45% are omnivores (Table 1) (Malcicka et al. 2017). Dietary compensation could, however, still play a role in the evolution of LA synthesis. Functional requirements for LA can be highly species-specific and information on dietary fatty acid profiles could prove extremely helpful in determining the role of dietary preferences in the evolution LA synthesis. We currently have insufficient information, however, on dietary fatty acid profiles for species known to lack or synthesize LA. Future research could aim to study dietary fatty acid profiles of closely related species that differ in their ability to synthesize LA. High species specificity could further explain why some invertebrates are able to synthesize LA despite feeding on a high-LA containing diet.
Another scenario under which organisms could obtain essential nutrients is via symbioses with microbes. Ecological interactions were recently found to play a critical role in fueling the loss of essential traits, i.e. compensated trait loss (Visser et al. 2010; Ellers et al. 2012). Here, an essential function or resource is provided by the environment, usually by a symbiotic partner, rendering the trait in the receiving organism either redundant or too costly to maintain, ensuing trait loss. When the ecological partner/interaction is absent, however, the functional requirement for the trait remains; hence fitness is negatively affected in absence of the ecological partner/interaction. Symbiotic partners, such as endosymbionts, may thus play a key role in the evolution of LA synthesis.
Perhaps one of the most thorough studies into the role of microorganisms in LA synthesis was done by Borgeson et al. (1991), in which several tissues of the house cricket Acheta domestica and the American cockroach Periplaneta americana were examined under axenic (i.e. endosymbiont-free) conditions. Their results unequivocally demonstrated that the insect tissues themselves, and not microorganisms, were responsible for the synthesis of LA. Employing a slightly different methodology, the role of endosymbionts in the pea aphid, Acyrthosiphon pisum, has also been studied (de Renobales et al. 1986). Aphids are known to harbor different species of microbes, some of which are essential for their survival (reviewed in Douglas 1998; Oliver et al. 2010). Nevertheless, tetracycline-treated aphids with greatly reduced numbers of intracellular symbionts synthesized LA in the same proportions as untreated controls (de Renobales et al. 1986). Several other studies on insects have provided additional evidence that microorganisms are not involved in LA synthesis (Wharton and Lola 1970; Mauldin et al. 1978; Dwyer et al. 1981; Blomquist et al. 1982; Stanley-Samuelson et al. 1986a; de Renobales et al. 1986; Jurenka et al. 1987; Borgeson et al. 1991) and we have yet to discover an example of endosymbiont-mediated LA synthesis. Moreover, the discovery of Δ12-desaturase genes in A. domesticus and T. castaneum (Zhou et al. 2008) supports the above findings that microorganisms are not mediating LA synthesis.
Utilization of LA by the Organism
Not all animal species have the same quantitative need for LA, as the biochemical and physiological pathways for which LA is used depend on the species’ biology and behavior. One of the most important functions of PUFAs is within cell membranes. Maintaining proper membrane fluidity is a crucial function of biological membranes in poikilothermic species, also known as homeoviscous adaptation (for review see Hazel 1995). This homeoviscous adaptation is achieved by desaturation of the phospholipid FA chain, which determines melting temperature and fluidity of biological membranes (Fromm and Hargrove 2012). Although homeoviscous adaptation is a very general mechanism in animals, it could be a driving force in the evolution of LA synthetic ability in organisms that regularly encounter cold temperatures or substantial temperature variation, leading to an increased demand for PUFAs. This would suggest LA synthesis to be more prevalent in temperate species encountering higher temperature variability, compared to species living in the tropics. The American and Japanese cockroaches, P. americana and P. japonica, for example, can synthesize (Z,Z)-6,9-heptacosadiene from LA, which remains as a cuticular hydrocarbon to prevent desiccation, protect against environmental chemicals, and serve as a pheromone, kairomone and defense chemical (Dwyer et al. 1981). The same compound was also found in the beetle (Tetropium cinnamopterum) and a hymenopteran (Ampulex compressa). It is unclear whether these species synthesize LA, but their phylogenetic position suggests the potential for de novo LA synthesis. The distribution of species living in temperate climates is, however, scattered on the phylogenetic tree with half of the species able to synthesize LA while the other half is not (Fig. 1). The phylogeny would thus suggest that adaptation to colder or more variable thermal environments may not be the main driving force underlying the evolution of LA synthesis. Moreover, some species, such as those regularly exposed to stressful environmental conditions, may require much higher quantities of LA compared to closely related species inhabiting more hospitable environments. For example, some species of yeast and algae were found to require more LA when exposed to lower temperature, higher salinity or nitrogen starvation (Gostinčar et al. 2009; Lu et al. 2009; Iskandarov et al. 2010; Chodok et al. 2013; Kaye et al. 2015).
Another role of LA is to modulate immune function through its effect on eicosanoid synthesis (Chuang et al. 2001; Eder et al. 2003). Eicosanoids are signaling molecules made by the enzymatic or non-enzymatic oxidation of PUFAs, which mediate specific cell actions, connect innate and adaptive immunity, and eicosanoids play a significant role in insect immune responses to bacterial, fungal and viral infections (Stanley-Samuelson et al. 1991; Harizi and Gualde 2005; Stanley et al. 2012; Büyükgüzel 2012; Park et al. 2012). Eicosanoids are also involved in egg-laying behaviors of some insects and vertebrates (Stanley 2006). For instance, the eicosanoid prostaglandin was found to mediate egg-laying behavior in some cricket species and to stimulate hatching behavior in barnacle larvae (Destephano and Brady 1977; Yamaja Setty and Ramaiah 1979; Stanley-Samuelson et al. 1986b; Stanley 2006).
A final possible way through which LA can be utilized is in the production of sex pheromones. LA has been identified as a critical component or precursor of sex pheromones in many insect species, particularly in Lepidoptera and Diptera, but also in some species of Coleoptera and Acari (Blomquist et al. 1987; Vanderwel and Oehlschlager 1987; Rule and Roelofs 1989; Blaul et al. 2014; Shimizu et al. 2014; Rong et al. 2015). In the mite Carpoglyphus lactis (capable of LA synthesis), for example, LA was converted to (Z,Z)-6,9-heptadecadiene, which is widely used as alarm, sex, and/or aggregation pheromone among astigmatid mites (Kuwahara 2004; Shimizu et al. 2014). A recent study further showed that the production of sex pheromones in Nasonia vitripennis males reared on a LA-enriched diet was four times higher than that of males reared on a minimal LA-containing diet; despite the fact this species is capable of LA synthesis (Brandstetter and Ruther 2016). Moths in the families Geometridae, Arctiidae, and Noctuidae also utilize LA as a precursor for pheromone production (Millar 2000). Although no record exists on de novo synthesis of LA in species from these families, their position within the phylogenetic tree (Fig. 1) would suggest that they cannot synthesize LA. The evolution of LA synthesis thus seems to be unrelated to pheromone production, as many species require LA as a precursor but cannot synthesize LA de novo (Table 1; Millar 2000).
Perspective on the Evolution of LA Synthesis
The question why some organisms possess the ability for LA synthesis and other, even closely related species, do not is still open. One potential explanation might be in the quantity of LA present in the diet relative to the organism’s requirements (Nugteren et al. 1979; Domenichiello et al. 2016). LA synthesis might also depend on the interplay between a species’ ecology (how much is in the diet) and the biological functions for which LA and its derivatives are required by a certain species. Some species (e.g. yeast, algae) may require much more LA than others, depending on environmental challenges such as stress caused by fluctuations in temperature, salinity or nitrogen availability (Gostinčar et al. 2009; Lu et al. 2009; Iskandarov et al. 2010; Chodok et al. 2013; Kaye et al. 2015). Other species may require a higher amount of LA because of the functions that LA performs in egg-laying behavior, innate immune reactions to infections (viral, bacterial, and fungal), the production of sex pheromones or in the performance and survival of immature stages (Stanley 2006; Park et al. 2012; Blaul et al. 2014; Brandstetter and Ruther 2016). Species that are unable to synthesize LA should complete these functions via other ways, such as through acquisition via the diet (Stanley 2006; Eleftherianos et al. 2013).
LA synthesis does not seem to evolve in response to a single function, but rather seems to evolve through a balance between the acquisition and requirement of LA. In terms of mechanisms, LA has clear pleiotropic functions, explaining why the metabolic machinery underlying LA synthesis remains in place and why it is easy to re-evolve this phenotypic function (although some organisms seem to lack the ∆12 gene) (Alonso et al. 2003). Pleiotropy could also explain occasions where LA synthesis might seem non-adaptive (i.e. the machinery remains due to evolutionary history, but the need for LA was lost for instance due to a change in environmental conditions). Furthermore, the dual function of some ∆12 desaturases (∆12/15 in C. elegans and L. kononenkoae) suggests that the underlying gene already has a novel function (Zhou et al. 2011; Yan et al. 2013). Work on desaturase genes indeed shows that there is rapid functional divergence even within subfamilies (Hashimoto et al. 2008). This might explain why even closely related species do not share the same mechanism behind LA synthesis and why it is hard to find any general evolutionary (phylogenetic) patterns of LA synthesis mechanism between plants, microorganism and/or invertebrates.
Conclusion
LA is involved in a wide array of biological functions and genes underlying LA synthesis show a high degree of divergence. We suggest future studies on the evolution of LA synthesis could focus on distinct taxonomic groups where closely related species vary in the ability to synthesize LA. Thorough investigation of the animal’s ecology (dietary FA profiles, sex pheromones, homeoviscous adaptation, stress and use of eicosanoids) and a search for underlying Δ12-desaturase genes could then reveal how LA acquisition and utilization interact to drive the evolution of LA synthesis.
References
Aboshi, T., Shimizu, N., Nakajima, Y., Honda, Y., Kuwahara, Y., Amano, H., & Mori, N. (2013). Biosynthesis of linoleic acid in Tyrophagus mites (Acarina: Acaridae). Insect Biochemistry and Molecular Biology Journal, 43, 991–996.
Alonso, D. L., Garcia-Maroto, F., Rodriguez-Ruiz, J., Garrido, J. A., & Vilches, M. A. (2003). Evolution of the membrane-bound fatty acid desaturases. Biochemical Systematics and Ecology, 31, 1111–1124.
Alonso, J., Schimpl, M., & van Aalten, D. M. (2014). O-GlcNAcase: Promiscuous hexosaminidase or key regulator of O-GlcNAc signaling? The Journal of Biological Chemistry, 289, 34433–34439.
Anderson, G. J., & Connor, W. E. (1989). On the demonstration of ω-3 essential-fatty-acid deficiency in humans. The American Journal of Clinical Nutrition, 49, 585–587.
Belury, M. A. (2002). Dietary conjugated linoleic acid in health: Physiological effects and mechanisms of action 1. Annual Review of Nutrition, 22, 505–531.
Blaul, B., Steinbauer, R., Merkl, P., Merkl, R., Tschochner, H., & Ruther, J. (2014). Oleic acid is a precursor of linoleic acid and the male sex pheromone in Nasonia vitripennis. Insect Biochemistry and Molecular Biology Journal, 51, 33–40.
Blomquist, G. J., Dwyer, L. A., Chu, A. J., Ryan, R. O., & de Renobales, M. (1982). Biosynthesis of linoleic acid in a termite, cockroach and cricket. Insect Biochemistry, 12, 349–353.
Blomquist, G. J., Nelson, D. R., & De Renobales, M. (1987). Chemistry, biochemistry, and physiology of insect cuticular lipids. Archives of Insect Biochemistry and Physiology, 6, 227–265.
Booth-Kewley, S., & Friedman, H. S. (1987). Psychological predictors of heart disease: A quantitative review. Psychological Bulletin, 101, 343.
Borgeson, C. E., Kurtti, T. J., Munderloh, U. G., & Blomquist, G. J. (1991). Insect tissues, not microorganisms, produce linoleic acid in the house cricket and the American cockroach. Experientia, 47, 238–241.
Brandstetter, B., & Ruther, J. (2016). An insect with a delta-12 desaturase, the jewel wasp Nasonia vitripennis, benefits from nutritional supply with linoleic acid. The Science of Nature, 103, 1–4.
Brock, G. R., & Chapple, I. L. (2016). The potential impact of essential nutrients vitamins C and D upon periodontal disease pathogenesis and therapeutic outcomes. Current Oral Health Reports, 3, 337–346.
Browse, J., & Somerville, C. (1991). Glycerolipid synthesis: Biochemistry and regulation. Annual Review of Plant Biology, 42, 467–506.
Buček, A., Matoušková, P., Sychrová, H., Pichová, I., & Hrušková-Heidingsfeldová, O. (2014). ∆12-Fatty acid desaturase from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids. PLoS ONE, 9, e93322.
Büyükgüzel, E. (2012). Eicosanoids mediate cellular immune response and phenoloxidase reaction to viral infection in adult Pimpla turionellae. Archives of Insect Biochemistry and Physiology, 81, 20–33.
Certik, M., & Shimizu, S. (1999). Biosynthesis and regulation of microbial polyunsaturated fatty acid production. Journal of Bioscience and Bioengineering, 87, 1–14.
Chatterjee, I. B. (1973). Evolution and the biosynthesis of ascorbic acid. Science, 182, 1271–1272.
Chodok, P., Eiamsa-ard, P., Cove, D. J., Quatrano, R. S., & Kaewsuwan, S. (2013). Identification and functional characterization of two ∆12-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens. Journal of Industrial Microbiology & Biotechnology, 40, 901–913.
Chuang, L. T., Leonard, A. E., Liu, J. W., Mukerji, P., Bray, T. M., & Huang, Y. S. (2001). Inhibitory effect of conjugated linoleic acid on linoleic acid elongation in transformed yeast with human elongase. Lipids, 36, 1099–1103.
Covello, P. S., & Reed, D. W. (1996). Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae. Plant Physiology, 111, 223–226.
Cripps, C., Blomquist, G. J., & de Renobales, M. (1986). De novo biosynthesis of linoleic acid in insects. Biochimica et Biophysica Acta (BBA: Lipids and Lipid Metabolism, 876, 572–580.
De Renobales, M., Ryan, R. O., Heisler, C. R., McLean, D. L., & Blomquist, G. J. (1986). Linoleic acid biosynthesis in the pea aphid, Acyrthosiphon pisum (Harris). Archives of Insect Biochemistry and Physiology, 3, 193–203.
De Veth, M. J., Bauman, D. E., Koch, W., Mann, G. E., Pfeiffer, A. M., & Butler, W. R. (2009). Efficacy of conjugated linoleic acid for improving reproduction: A multi-study analysis in early-lactation dairy cows. Journal of Dairy Science, 92, 2662–2669.
Destephano, D. B., & Brady, U. E. (1977). Prostaglandin and prostaglandin synthetase in the cricket, Acheta domesticus. Journal of Insect Physiology, 23, 905–911.
Domenichiello, A. F., Kitson, A. P., Chen, C. T., Trépanier, M. O., Stavro, P. M., & Bazinet, R. P. (2016). The effect of linoleic acid on the whole body synthesis rates of polyunsaturated fatty acids from α-linolenic acid and linoleic acid in free-living rats. The Journal of Nutritional Biochemistry, 30, 167–176.
Douglas, A. E. (1998). Nutritional interactions in insect-microbial symbioses: Aphids and their symbiotic bacteria Buchnera. Annual Review of Entomology, 43, 17–37.
Dwyer, L. A., Blomquist, G. J., Nelson, J. H., & George Pomonis, J. (1981). A sup 13sup C-NMR study of the biosynthesis of 3-methylpentacosane in the American cockroach. Biochimica et Biophysica Acta (BBA): Lipids and Lipid Metabolism, 663, 536–544.
Eder, K., Schleser, S., Becker, K., & Körting, R. (2003). Conjugated linoleic acids lower the release of eicosanoids and nitric oxide from human aortic endothelial cells. The Journal of Nutrition, 133, 4083–4089.
Eleftherianos, I., Atri, J., Accetta, J., & Castillo, J. (2013). Endosymbiotic bacteria in insects: Guardians of the immune system? Frontiers in Physiology 4, 46.
Ellers, J., Kiers, Toby, Currie, E., McDonald, C. R., Visser, B. R., B (2012). Ecological interactions drive evolutionary loss of traits. Ecology Letters, 15, 1071–1082.
El-Yassimi, A., Hichami, A., Besnard, P., & Khan, N. A. (2008). Linoleic acid induces calcium signaling, Src kinase phosphorylation, and neurotransmitter release in mouse CD36-positive gustatory cells. The Journal of Biological Chemistry, 283, 12949–12959.
Farmer, E. E. (1994). Fatty acid signalling in plants and their associated microorganisms. Plant Molecular Biology, 26, 1423–1437.
Fromm, H. J., & Hargrove, M. (2012). Essentials of biochemistry. Berlin: Springer.
Gostinčar, C., Turk, M., Plemenitaš, A., & Gunde-Cimerman, N. (2009). The expressions of ∆9-, ∆12-desaturases and an elongase by the extremely halotolerant black yeast Hortaea werneckii are salt dependent. FEMS Yeast Research, 9, 247–256.
Grosso, G., Micek, A., Marventano, S., Castellano, S., Mistretta, A., Pajak, A., & Galvano, F. (2016). Dietary n-3 PUFA, fish consumption and depression: A systematic review and meta-analysis of observational studies. Journal of Affective Disorders, 205, 269–281.
Harizi, H., & Gualde, N. (2005). The impact of eicosanoids on the crosstalk between innate and adaptive immunity: The key roles of dendritic cells. Tissue Antigens, 65, 507–514.
Hashimoto, K., Yoshizawa, A. C., Okuda, S., Kuma, K., Goto, S., & Kanehisa, M. (2008). The repertoire of desaturases and elongases reveals fatty acid variations in 56 eukaryotic genomes. The Journal of Lipid Research, 49, 183–191.
Hazel, J. R. (1995). Thermal adaptation in biological membranes: Is homeoviscous adaptation the explanation? Annual Review of Physiology, 57, 19–42.
Helliwell, K. E., Wheeler, G. L., & Smith, A. G. (2013). Widespread decay of vitamin-related pathways: Coincidence or consequence? Trends in Genetics: TIG, 29, 469–478.
Horrobin, D. F. (1998). The membrane phospholipid hypothesis as a biochemical basis for the neurodevelopmental concept of schizophrenia. Schizophrenia Research, 30, 193–208.
Hulbert, A. J., Turner, N., Storlien, L. H., & Else, P. L. (2005). Dietary fats and membrane function: Implications for metabolism and disease. Biological Review, 80, 155–169.
Iskandarov, U., Khozin-Goldberg, I., & Cohen, Z. (2010). Identification and characterization of ∆12, ∆6, and ∆5 desaturases from the green microalga Parietochloris incisa. Lipids, 45, 519–530.
Jenkins, N. D., Buckner, S. L., Baker, R. B., Bergstrom, H. C., Cochrane, K. C., Weir, J. P., … Cramer, J. T. (2014). Effects of 6 weeks of aerobic exercise combined with conjugated linoleic acid on the physical working capacity at fatigue threshold. The Journal of Strength & Conditioning Research, 28, 2127–2135.
Jurenka, R. A., de Renobales, M., & Blomquist, G. J. (1987). De novo biosynthesis of polyunsaturated fatty acids in the cockroach Periplaneta americana. Archives of Biochemistry and Biophysics, 255, 184–193.
Kainou, K., Kamisaka, Y., Kimura, K., & Uemura, H. (2006). Isolation of ∆12 and ω3-fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and α-linolenic acids in Saccharomyces cerevisiae. Yeast (Chichester, England), 23, 605–612.
Kalmijn, S., Feskens, E. J. M., Launer, L. J., & Kromhout, D. (1997). Polyunsaturated fatty acids, antioxidants, and cognitive function in very old men. American Journal of Epidemiology, 145, 33–41.
Kaye, Y., Grundman, O., Leu, S., Zarka, A., Zorin, B., Didi-Cohen, S., Khozin-Goldberg, I., & Boussiba, S. (2015). Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: Overexpression of endogenous ∆12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG. Algal Research, 11, 387–398.
Kennedy, D. O. (2016). B vitamins and the brain: Mechanisms, dose and efficacy—A review. Nutrients, 8, 68.
Kuwahara, Y. (2004). Chemical ecology of astigmatid mites. Advance Insect Chemical Ecology, 65, 76–109.
Los, D. A., & Murata, N. N (1998). Structure and expression of fatty acid desaturases. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1394, 3–15.
Louloudes, S. J., Kaplanis, J. N., Robbins, W. E., & Monroe, R. E. (1961). Lipogenesis from C14-acetate by the American cockroach. Annals of the Entomological Society of America, 54, 99–103.
Lu, Y., Chi, X., Yang, Q., Li, Z., Liu, S., Gan, Q., & Qin, S. 2009. Molecular cloning and stress-dependent expression of a gene encoding ∆12-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7. Extremophiles, 13, 875–884.
Macartney, A., Maresca, B., & Cossins, A. R. (1994). Acyl-CoA desaturases and the adaptive regulation of membrane lipid composition. Temperature adaptation of biological membranes. London: Portland Press.
Malcicka, M., Ruther, J., & Ellers, J. (2017). De novo synthesis of linoleic acid in multiple Collembola species. Journal of Chemical Ecology, 9, 911–919.
Mauldin, J. K., Rich, N. M., & Cook, D. W. (1978). Amino acid synthesis from 14C-acetate by normally and abnormally faunated termites, Coptotermes formosanus. Insect Biochemistry, 8, 105–109.
Mauldin, J. K., Smythe, R. V., & Baxter, C. C. (1972). Cellulose catabolism and lipid synthesis by the subterranean termite, Coptotermes formosanus. Insect Biochemisty, 2, 209–217.
Millar, J. G. (2000). Polyene hydrocarbons and epoxides: A second major class of lepidopteran sex attractant pheromones. Annual Review of Entomology, 45, 575–604.
Murata, N., & Wada, H. (1995). Acyl-lipid desaturases and their importance in the tolerance and acclimatization to cold of cyanobacteria. The Biochemical Journal, 308, 1–6.
Nugteren, D. H., Van Evert, W. C., Soeting, W. J., & Spuy, J. H. (1979). Effect of different amounts of linoleic acid in the diet on the excretion of urinary prostaglandin metabolites in the rat. Advances in Prostaglandin and Thromboxane Research, 8, 1793–1796.
Ohta, Y., & Nishikimi, M. (1999). Random nucleotide substitutions in primate nonfunctional gene for l-gulono-γ-lactone oxidase, the missing enzyme in l-ascorbic acid biosynthesis. Biochimica et Biophysica Acta (BBA): General Subjects, 1472, 408–411.
Oliver, K. M., Degnan, P. H., Burke, G. R., & Moran, N. A. (2010). Facultative symbionts in aphids and the horizontal transfer of ecologically important traits. Annual Review of Entomology, 55, 247–266.
Palmquist, D. L., Lock, A. L., Shingfield, K. J., & Bauman, D. E. (2005). Biosynthesis of conjugated linoleic acid in ruminants and humans. Advances in Food and Nutrition Research, 50, 179–217.
Pandey, V. C., Prakash, P., Bajpai, O., Kumar, A., & Singh, N. (2015). Phytodiversity on fly ash deposits: Evaluation of naturally colonized species for sustainable phytorestoration. Environmental Science and Pollution Research, 22, 2776–2787.
Park, W. B., Kim, N. H., Kim, K. H., Lee, S. H., Nam, W. S., Yoon, S. H., Song, K. H., Choe, P. G., Kim, N. J., Jang, I. J., & Oh, M. D. (2012). The effect of therapeutic drug monitoring on safety and efficacy of voriconazole in invasive fungal infections: A randomized controlled trial. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 55, 1080–1087.
Peyou-Ndi, M. M., Watts, J. L., & Browse, J. (2000). Identification and characterization of an animal ∆ 12 fatty acid desaturase gene by heterologous expression in Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics, 376, 399–408.
Qi, B., Fraser, T., Mugford, S., Dobson, G., Sayanova, O., Butler, J., … Lazarus, C. M. (2004). Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nature Biotechnology, 22, 739–745.
Rong, J., Liu, N. Y., Yan, L., & Dong, S. L. (2015). A larval specific OBP able to bind the major female sex pheromone component in Spodoptera exigua (Hübner). Journal of Integrative Agriculture, 14, 1356–1366.
Rule, G. S., & Roelofs, W. L. (1989). Biosynthesis of sex pheromone components from linolenic acid in arctiid moths. Archives of Insect Biochemistry and Physiology, 12, 89–97.
Sampath, H., & Ntambi, J. M. (2005). Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annual Review of Nutrition, 25, 317–340.
Shimizu, N., Naito, M., Mori, N., & Kuwahara, Y. (2014). De novo biosynthesis of linoleic acid and its conversion to the hydrocarbon (Z, Z)-6, 9-heptadecadiene in the astigmatid mite, Carpoglyphus lactis: Incorporation experiments with 13C-labeled glucose. Insect Biochemistry and Molecular Biology, 45, 51–57.
Sinclair, A. J., Begg, D., Mathai, M., & Weisinger, R. S. (2007). Omega 3 fatty acids and the brain: Review of studies in depression. Asia Pacific Journal of Clinical Nutrition, 16, 391–397.
Stanley, D. (2006). Prostaglandins and other eicosanoids in insects: Biological significance. Annual Review of Entomology, 51, 25–44.
Stanley, W. C., Khairallah, R. J., & Dabkowski, E. R. (2012). Update on lipids and mitochondrial function: Impact of dietary n-3 polyunsaturated fatty acids. Current Opinion in Clinical Nutrition and Metabolic Care, 15, 122.
Stanley-Samuelson, D. W., Jensen, E., Nickerson, K. W., Tiebel, K., Ogg, C. L., & Howard, R. W. (1991). Insect immune response to bacterial infection is mediated by eicosanoids. Proceedings of the National Academy of Sciences, 88, 1064–1068.
Stanley-Samuelson, D. W., Loher, W., & Blomquist, G. J. (1986a). Biosynthesis of polyunsaturated fatty acids by the Australian field cricket, Teleogryllus commodus. Insect Biochemistry, 16, 387–393.
Stanley-Samuelson, D. W., Peloquin, J. J., & Loher, W. (1986b). Egg-laying in response to prostaglandin injections in the Australian field cricket, Teleogryllus commodus. Physiological Entomology, 11, 213–219.
Thelen, J. J., & Ohlrogge, J. B. (2002). Metabolic engineering of fatty acid biosynthesis in plants. Metabolic Engineering, 4, 12–21.
Vanderwel, D., & Oehlschlager, A. C. (1987). Biosynthesis of pheromones and endocrine regulation of pheromone production in Coleoptera. In Pheromone biochemistry. New York: Academic Press.
Visser, B., & Ellers, J. (2008). Lack of lipogenesis in parasitoids: A review of physiological mechanisms and evolutionary implications. Journal of Insect Physiology, 54, 1315–1322.
Visser, B., Le Lann, C., den Blanken, F. J., Harvey, J. A., van Alphen, J. J., & Ellers, J. (2010). Loss of lipid synthesis as an evolutionary consequence of a parasitic lifestyle. Proceedings of the National Academy of Sciences, 107, 8677–8682.
Wallis, J. G., Watts, J. L., & Browse, J. (2002). Polyunsaturated fatty acid synthesis: What will they think of next? Trends in Biochemical Sciences, 27, 467–473.
Watts, J., & Browse, L. J. (2002). Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans. Proceedings of the National Academy of Sciences, 99, 5854–5859.
Weber, H. (2002). Fatty acid-derived signals in plants. Trends in Plant Science, 7, 217–224.
Weinert, J., Blomquist, G. J., & Borgeson, C. E. (1993). De novo biosynthesis of linoleic acid in two non-insect invertebrates: The land slug and the garden snail. Experientia, 49, 919–921.
Wharton, D. R. A., & Lola, J. E. (1970). Blood conditions and lysozyme action in the aposymbiotic cockroach. Journal of Insect Physiology, 16, 199–209.
Yamaja Setty, B. N., & Ramaiah, T. R. (1979). Isolation and identification of prostaglandins from the reproductive organs of male silkmoth, Bombyx mori L. Insect Biochemistry, 9, 613–617.
Yan, Z., Zhuo, L., Mulan, J., Xia, W., Yangmin, G., Yinbo, Z., & Fenghong, H. (2013). Clone and identification of bifunctional ∆12/∆15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae. Food Science and Biotechnology, 22, 573–576.
Zhou, X. R., Horne, I., Damcevski, K., Haritos, V., Green, A., & Singh, S. (2008). Isolation and functional characterization of two independently-evolved fatty acid ∆12-desaturase genes from insects. Insect Molecular Biology, 17, 667–676.
Zhou, X.-R., Green, A. G., & Singh, S. P. (2011). Caenorhabditis elegans ∆12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the ∆12 and ∆15 positions. The Journal of Biological Chemistry, 286, 43644–43650.
Acknowledgements
This research was supported by The Netherlands Organization for Scientific Research [NWO, VICI, (Stichting voor de Technische Wetenschappen) Grant 865.12.003] and the Fonds de la Recherche Scientifique—FNRS (Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture) under Grant Nos. 24905063 and 29109376. This is publication BRC 399 of the Biodiversity Research Centre.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Malcicka, M., Visser, B. & Ellers, J. An Evolutionary Perspective on Linoleic Acid Synthesis in Animals. Evol Biol 45, 15–26 (2018). https://doi.org/10.1007/s11692-017-9436-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11692-017-9436-5