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Clone and identification of bifunctional Δ12/Δ15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae

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Abstract

In modern diet of human being, the ω-6/ω-3 ratio of dietary fatty acid was shifted much towards ω-6 series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Δ12/Δ15 bifunctional FAD which could not only produce linoleic acid and α-linolenic acid use just oleic acid as substrate but also adjust the ω- 6/ω-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei Province, 430062, China

    Zhang Yan, Liang Zhuo, Jiang Mulan, Wan Xia, Gon Yangmin, Zhang Yinbo & Huang Fenghong

  2. College of Food Science and Technology, Huazhong Agriculture University, Wuhan, Hubei Province, 430070, China

    Zhang Yan & Huang Fenghong

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  1. Zhang Yan
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  2. Liang Zhuo
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  3. Jiang Mulan
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  4. Wan Xia
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  5. Gon Yangmin
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  6. Zhang Yinbo
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  7. Huang Fenghong
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Correspondence to Huang Fenghong.

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Yan, Z., Zhuo, L., Mulan, J. et al. Clone and identification of bifunctional Δ12/Δ15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae . Food Sci Biotechnol 22, 573–576 (2013). https://doi.org/10.1007/s10068-013-0116-7

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  • DOI: https://doi.org/10.1007/s10068-013-0116-7

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