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Inhibitory effect of conjugated linoleic acid on linoleic acid elongation in transformed yeast with human elongase

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Lipids

Abstract

Conjugated linoleic acid (CLA; 18∶2) refers to a group of positional and geometric isomers derived from linoleic acid (LA; Δ9, 12–18∶2). Using a growing baker's yeast (Saccharomyces cerevisiae) transformed with human elongase gene, we examined the inhibitory effect of CLA at various concentrations (10, 25, 50, and 100 μM) on elongation of LA (25 μM) to eicosadienoic acid (EDA; Δ11,14–20∶2). Among four available individual CLA isomers, only c9,t11- and t10,c12-isomers inhibited elongation of LA to EDA. The extent of inhibition (ranging from 20 to 60%) was related to the concentration of CLA added to the medium. In the meantime, only these two isomers, when added at 50 μM to the media, were elongated to conjugated EDA (c11,t13- and t12,c14–20∶2) by the same recombinant elongase at the rate of 28 and 24%, respectively. The inhibitory effect of CLA on LA elongation is possibly due to competition between CLA isomers and LA for the recombinant elongase. Thus, results from this study and a previous study suggest that the biological effect of CLA is exerted through its inhibitory effect on Δ6-desaturation as well as elongation of LA which results in a decrease in long-chain n−6 fatty acids and consequently the eicosanoid synthesis.

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Abbreviations

AA:

arachidonic acid (Δ5,8,11,14–20∶4)

CEDA:

conjugated eicosadienoic acid

CLA:

conjugated linoleic acid

CSM-LEU:

complete supplement mixture minus leucine

DGLA:

dinomo-γ-linolenic acid (Δ8,11,14–20∶3)

DOB:

Dropout base

EDA:

eicosadienoic acid (Δ11,14–20∶2)

GC:

gas chromatography

GLA:

γ-linolenic acid (Δ6,9,12–18∶3)

LA:

linoleic acid (Δ9,12–18∶2)

MS:

mass spectrometry

PUFA:

polyunsaturated fatty acid

YMM:

yeast minimal medium

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Authors and Affiliations

  1. Department of Human Nutrition and Food Management, The Ohio State University, 43210, Columbus, Ohio

    Lu-Te Chuang, Tammy M. Bray & Yung-Sheng Huang

  2. Strategic-Discovery R&D, Ross Products Division, Abbott Laboratories, 625 Cleveland Ave., 43215, Columbus, OH

    Lu-Te Chuang, Amanda E. Leonard, Jim-Wen Liu, Pradip Mukerji & Yung-Sheng Huang

Authors
  1. Lu-Te Chuang
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  2. Amanda E. Leonard
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  3. Jim-Wen Liu
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  4. Pradip Mukerji
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  5. Tammy M. Bray
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  6. Yung-Sheng Huang
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Correspondence to Yung-Sheng Huang.

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Chuang, LT., Leonard, A.E., Liu, JW. et al. Inhibitory effect of conjugated linoleic acid on linoleic acid elongation in transformed yeast with human elongase. Lipids 36, 1099–1103 (2001). https://doi.org/10.1007/s11745-001-0819-0

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  • DOI: https://doi.org/10.1007/s11745-001-0819-0

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