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Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants

  • Developmental Biology/Morphogenesis
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Summary

An efficient regeneration system was developed by culturing immature cotyledons and embryo axes of Arachis hypogaea L. cv. Georgia Green on Murashige and Skoog basal medium (MS) supplemented with various concentrations of thidiazuron (TDZ; 1, 5, 10, and 15 µM). Highly morphogenic callus was produced from 100% of the explants comprising the cotyledon with attached embryo axis when cultured in the dark on 10 µM TDZ. Upon excision and continued culture in the dark on 10 µM TDZ, morphogenic callus grew repetitively during monthly subcultures and retained its regeneration potential. For organogenesis, a gradual reduction in TDZ concentration and exposure to light were necessary before transfer to MS basal medium. Inclusion of indole-3-butyric acid in liquid MS medium favored rooting of recovered shoots. A distinct feature of this investigation is the induction of highly morphogenic callus by TDZ and regeneration of morphologically normal, fertile peanut plants after 8 months of callus subculture.

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Authors and Affiliations

  1. Department of Horticulture, The University of Georgia, Coastal Plain Experiment Station, 31793-0748, Tifton, Georgia

    R. Gill & P. Ozias-Akins

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  1. R. Gill
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  2. P. Ozias-Akins
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Correspondence to P. Ozias-Akins.

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Gill, R., Ozias-Akins, P. Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants. In Vitro Cell.Dev.Biol.-Plant 35, 445–450 (1999). https://doi.org/10.1007/s11627-999-0066-1

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  • DOI: https://doi.org/10.1007/s11627-999-0066-1

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